1921
Volume 83, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

More than 5,000 diarrheal cases per year receive medical care at the National Children's Hospital of Costa Rica, and nearly 5% of them require hospitalization. A total of 173 strains isolated from children with diarrhea were characterized at the molecular, serologic, and phenotypic level. Multiplex and duplex polymerase chain reactions were used to detect the six categories of diarrheagenic . Thirty percent (n = 52) of the strains were positive, indicating a high prevalence among the pediatric population. Enteropathogenic and enteroinvasive pathotypes were the most prevalent (21% and 19%, respectively). Pathogenic strains were distributed among the four phylogenetic groups A, B1, B2, and D, with groups A and B1 the most commonly found. This study used molecular typing to evaluate the prevalence of diarrheagenic reported in Costa Rica and demonstrated the importance of these pathotypes in the pediatric population.

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2010-08-05
2017-09-22
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  • Received : 20 Jul 2009
  • Accepted : 16 May 2010

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