1921
Volume 82, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Monkeypox virus (MPXV), a member of the family and genus , causes a smallpox-like disease in humans. A previously described pan- assay, based on a broad-range polymerase chain reaction (PCR) coupled with electrospray ionization mass spectrometry (PCR/ESI-MS), was evaluated for its ability to detect MPXV from spiked human and aerosol-infected cynomolgous macaque () samples. Detection of MPXV DNA from macaque tissue, blood, and spiked human blood by the PCR/ESI-MS pan- assay was comparable, albeit at slightly higher levels, to the current gold standard method of real-time PCR with the pan- assay and had a limit of detection of 200 plaque-forming units. Furthermore, the platform was able to distinguish MPXV and vaccinia viruses that were spiked into macaque blood samples at various concentrations. This platform provides a new tool for the diagnosis and monitoring of orthopoxviral loads during vaccine or antiviral studies, but also could provide rapid identification during natural outbreaks or bioterrorism attacks.

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2010-02-01
2017-11-23
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References

  1. Eyler JM, , 2003. Smallpox in history: the birth, death, and impact of a dread disease. J Lab Clin Med 142: 216220.[Crossref]
  2. Jezek Z, Fenner F, , 1988. Human Monkeypox. Basel: Karger.
  3. Jezek Z, Grab B, Paluku KM, Szczeniowski MV, , 1988. Human monkeypox: disease pattern, incidence and attack rates in a rural area of northern Zaire. Trop Geogr Med 40: 7383.
  4. Ladnyi ID, Ziegler P, Kima E, , 1972. A human infection caused by monkeypox virus in Basankusu Territory, Democratic Republic of the Congo. World Health Organ 46: 593597.
  5. Hutin YJ, Williams RJ, Malfait P, Pebody R, Loparev VN, Ropp SL, Rodriguez M, Knight JC, Tshioko FK, Khan AS, Szczeniowski MV, Esposito JJ, , 2001. Outbreak of human monkeypox, Democratic Republic of Congo, 1996 to 1997. Emerg Infect Dis 7: 434438.[Crossref]
  6. Magnus P, von Anderson EK, Petersen KB, Birch-Andersen A, , 1959. A pox-like disease in cynomolgus monkeys. Acat Path Microbiol Scand 46: 156176.[Crossref]
  7. Kulesh DA, Loveless BM, Norwood D, Garrison J, Whitehouse CA, Hartmann C, Mucker E, Miller D, Wasieloski LP, Jr Huggins J, Huhn G, Miser LL, Imig C, Martinez M, Larsen T, Rossi CA, Ludwig GV, , 2004. Monkeypox virus detection in rodents using real-time 3′-minor groove binder TaqMan assays on the Roche LightCycler. Lab Invest 84: 12001208.[Crossref]
  8. Reed KD, Melski JW, Graham MB, Regnery RL, Sotir MJ, Wegner MV, Kazmierczak JJ, Stratman EJ, Li Y, Fairley JA, Swain GR, Olson VA, Sargent EK, Kehl SC, Frace MA, Kline R, Foldy SL, Davis JP, Damon IK, , 2004. The detection of monkeypox in humans in the Western Hemisphere. N Engl J Med 350: 342350.[Crossref]
  9. Magnus PV, Anderson EK, Petersen KB, Birch-Andersen A, , 1959. A pox-like disease in cynomolgus monkeys. Acat Path Microbiol Scand 46: 156176.[Crossref]
  10. Chen N, Li G, Liszewski MK, Atkinson JP, Jahrling PB, Feng Z, Schriewer J, Buck C, Wang C, Lefkowitz EJ, Esposito JJ, Harms T, Damon IK, Roper RL, Upton C, Buller RM, , 2005. Virulence differences between monkeypox virus isolates from west Africa and the Congo basin. Virology 340: 4663.[Crossref]
  11. Li Y, Olson VA, Laue T, Laker MT, Damon IK, , 2006. Detection of monkeypox virus with real-time PCR assays. J Clin Virol 36: 194203.[Crossref]
  12. Sbrana E, Jordan R, Hruby DE, Mateo RI, Xiao SY, Siirin M, Newman PC, AP DAR, Tesh RB, , 2007. Efficacy of the antipoxvirus compound ST-246 for treatment of severe orthopoxvirus infection. Am J Trop Med Hyg 76: 768773.
  13. Stittelaar KJ, Neyts J, Naesens L, van Amerongen G, van Lavieren RF, Holy A, De Clercq E, Niesters HG, Fries E, Maas C, Mulder PG, van der Zeijst BA, Osterhaus AD, , 2006. Antiviral treatment is more effective than smallpox vaccination upon lethal monkeypox virus infection. Nature 439: 745748.[Crossref]
  14. Stittelaar KJ, van Amerongen G, Kondova I, Kuiken T, van Lavieren RF, Pistoor FH, Niesters HG, van Doornum G, van der Zeijst BA, Mateo L, Chaplin PJ, Osterhaus AD, , 2005. Modified vaccinia virus Ankara protects macaques against respiratory challenge with monkeypox virus. J Virol 79: 78457851.[Crossref]
  15. Parker S, Touchette E, Oberle C, Almond M, Robertson A, Trost LC, Lampert B, Painter G, Buller RM, , 2008. Efficacy of therapeutic intervention with an oral ether-lipid analogue of cidofovir (CMX001) in a lethal mousepox model. Antiviral Res 77: 3949.[Crossref]
  16. Hofstadler SA, Sampath R, Blyn LB, Eshoo MW, Hall TA, Jiang Y, Drader JJ, Hannis JC, Sannes-Lowery KA, Cummins LL, Libby B, Walcott DJ, Schink A, Massire C, Ranken R, White N, Samant V, McNeil JA, Knize D, Robbins D, Rudnick K, Desai A, Moradi E, Ecker DJ, , 2005. TIGER: the universal biosensor. Int J Mass Spectrom 242: 2341.[Crossref]
  17. Ecker DJ, Sampath R, Blyn LB, Eshoo MW, Ivy C, Ecker JA, Libby B, Samant V, Sannes-Lowery KA, Melton RE, Russell K, Freed N, Barrozo C, Wu J, Rudnick K, Desai A, Moradi E, Knize DJ, Robbins DW, Hannis JC, Harrell PM, Massire C, Hall TA, Jiang Y, Ranken R, Drader JJ, White N, McNeil JA, Crooke ST, Hofstadler SA, , 2005. Rapid identification and strain-typing of respiratory pathogens for epidemic surveillance. Proc Natl Acad Sci U S A 102: 80128017.[Crossref]
  18. Ecker DJ, Drader J, Gutierrez J, Gutierrez A, Hannis J, Schink A, Sampath R, Ecker AJ, Blyn LB, Eshoo MW, Hall TA, Tobarmosquera M, Jiang Y, Sannes-Lowery K, Cummins L, Libby B, Walcott DJ, Massire C, Ranken R, Manalili SM, Ivy C, Melton R, Levene H, Harpin V, Li F, White N, Pear M, Samant V, Knize DJ, Robbins DW, Rudnick K, Hajjar F, Hofstadler SA, , 2006. The Ibis T5000 universal biosensor: an automated platform for pathogen identification and strain typing. JALA 11: 341351.
  19. Sampath R, Russell KL, Massire C, Eshoo MW, Harpin V, Blyn LB, Melton R, Ivy C, Pennella T, Li F, Levene H, Hall TA, Libby B, Fan N, Walcott DJ, Ranken R, Pear M, Schink A, Gutierrez J, Drader J, Moore D, Metzgar D, Addington L, Rothman R, Gaydos CA, Yang S, St George K, Fuschino ME, Dean AB, Stallknecht DE, Goekjian G, Yingst S, Monteville M, Saad MD, Whitehouse CA, Baldwin C, Rudnick KH, Hofstadler SA, Lemon SM, Ecker DJ, , 2007. Global surveillance of emerging influenza virus genotypes by mass spectrometry. PLoS One 2: e489.[Crossref]
  20. Eshoo MW, Whitehouse CA, Zoll ST, Massire C, Pennella TT, Blyn LB, Sampath R, Hall TA, Ecker JA, Desai A, Wasieloski LP, Li F, Turell MJ, Schink A, Rudnick K, Otero G, Weaver SC, Ludwig GV, Hofstadler SA, Ecker DJ, , 2007. Direct broad-range detection of alphaviruses in mosquito extracts. Virology 368: 286295.[Crossref]
  21. Sampath R, Hofstadler SA, Blyn LB, Eshoo MW, Hall TA, Massire C, Levene HM, Hannis JC, Harrell PM, Neuman B, Buchmeier MJ, Jiang Y, Ranken R, Drader JJ, Samant V, Griffey RH, McNeil JA, Crooke ST, Ecker DJ, , 2005. Rapid identification of emerging pathogens: coronavirus. Emerg Infect Dis 11: 373379.[Crossref]
  22. Eshoo MW, Whitehouse CA, Nalca A, Zoll S, Ecker JA, Hall TA, Pennella TT, Duncan DD, Desai A, Moradi EK, R, Rudnick K, Libby B, Ranken R, Sampath R, Hofstadler SA, Ecker DJ, Blyn LB, , 2009. Rapid and high-throughput pan-Orthopoxvirus detection and identification by broad-range PCR and mass spectrometry. PloS One 4: e6342.[Crossref]
  23. Meyer H, Pfeffer M, Rziha HJ, , 1994. Sequence alterations within and downstream of the A-type inclusion protein genes allow differentiation of Orthopoxvirus species by polymerase chain reaction. J Gen Virol 75: 19751981.[Crossref]
  24. Ropp SL, Jin Q, Knight JC, Massung RF, Esposito JJ, , 1995. PCR strategy for identification and differentiation of small pox and other orthopoxviruses. J Clin Microbiol 33: 20692076.
  25. Kulesh DA, Baker RO, Loveless BM, Norwood D, Zwiers SH, Mucker E, Hartmann C, Herrera R, Miller D, Christensen D, Wasieloski LP, Jr Huggins J, Jahrling PB, , 2004. Smallpox and pan-orthopox virus detection by real-time 3′-minor groove binder TaqMan assays on the roche LightCycler and the Cepheid smart Cycler platforms. J Clin Microbiol 42: 601609.[Crossref]
  26. Muddiman DC, Anderson GA, Hofstadler SA, Smith RD, , 1997. Length and base composition of PCR-amplified nucleic acids using mass measurements from electrospray ionization mass spectrometry. Anal Chem 69: 15431549.[Crossref]
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  • Received : 25 Jun 2009
  • Accepted : 28 Sep 2009

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