Volume 82, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Monkeypox virus (MPXV), a member of the family and genus , causes a smallpox-like disease in humans. A previously described pan- assay, based on a broad-range polymerase chain reaction (PCR) coupled with electrospray ionization mass spectrometry (PCR/ESI-MS), was evaluated for its ability to detect MPXV from spiked human and aerosol-infected cynomolgous macaque () samples. Detection of MPXV DNA from macaque tissue, blood, and spiked human blood by the PCR/ESI-MS pan- assay was comparable, albeit at slightly higher levels, to the current gold standard method of real-time PCR with the pan- assay and had a limit of detection of 200 plaque-forming units. Furthermore, the platform was able to distinguish MPXV and vaccinia viruses that were spiked into macaque blood samples at various concentrations. This platform provides a new tool for the diagnosis and monitoring of orthopoxviral loads during vaccine or antiviral studies, but also could provide rapid identification during natural outbreaks or bioterrorism attacks.


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  • Received : 25 Jun 2009
  • Accepted : 28 Sep 2009
  • Published online : 05 Feb 2010

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