Volume 82, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Antibody (IgG) responses to the saliva of were investigated using serum samples from regions of India endemic and non-endemic for visceral leishmaniasis (VL). By pre-adsorbing the sera against the saliva of the competing human-biting but non-VL vector , we significantly improved the specificity of a saliva enzyme-linked immunosorbent assay. Using this method, we observed a statistically significant correlation between antibodies to saliva and the average indoor density of female sand flies. Additionally, the method was able to detect recent changes in vector exposure when sera from VL patients were assayed before, during, and after hospitalization and protected from sand fly bites under untreated bed nets. Collectively, these results highlight the utility of antibodies to saliva as an important tool to evaluate VL vector control programs.


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Supplementary Table 1.

  • Received : 17 Jun 2009
  • Accepted : 25 Oct 2009
  • Published online : 05 May 2010

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