Volume 82, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



We sought to test the association of polymorphisms in (, gene PF13_0019) with susceptibility to quinine, which was previously reported in a limited number of reference strains or culture-adapted isolates. Determination of susceptibility to quinine, genotyping of ms4760 microsatellite and polymorphism in codon 76 of were performed for 83 isolates obtained from symptomatic malaria-infected travelers returning from various African countries to France or from subjects living in Madagascar. Nineteen different ms4760 microsatellite profiles of were found including 14 not previously described. Multivariate analysis showed no significant association between the susceptibility to quinine with particular ms4760 profiles. Contrary to previous reports, we only observed that the number of NHNDNHNNDDD repeats was positively associated with the increased IC50 of QN ( = 0.01). We concluded that the studied polymorphisms in did not appear as valid molecular markers of susceptibility to quinine in isolates from Africa. Because we did not include any isolate of Asian origin in our series, these results did not exclude the possibility of regional associations, for example in South-East Asia.


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  1. Neiva A, , 1910. Ueber die Bildung einur chininresistenten Rasse des Malariaparasiten. Mem Inst Oswaldo Cruz 2: 131140.[Crossref] [Google Scholar]
  2. Nocht B, Werner H, , 1910. Beobachtungen uber eine relative Chininresistenz bei malaria aus Brasilien. Dtsch Med Wochenschr 36: 15571560.[Crossref] [Google Scholar]
  3. Peters W, , 1987. Resistance of human malaria I, III, and IV. IN: Chemotherapy and Drug Resistance in Malaria. Second edition. London: Academic Press, 543568, 593–658, 659–786. [Google Scholar]
  4. Wongsrichanalai C, Pickard AL, Wernsdorfer WH, Meshnick SR, , 2002. Epidemiology of drug-resistant malaria. Lancet Infect Dis 2: 209218.[Crossref] [Google Scholar]
  5. Ferdig MT, Cooper RA, Mu J, Deng B, Joy DA, Su XZ, Wellems TE, , 2004. Dissecting the loci of low-level quinine resistance in malaria parasites. Mol Microbiol 52: 985997.[Crossref] [Google Scholar]
  6. Nkrumah LJ, Riegelphaupt PM, Moura P, Johnson DJ, Patel J, Hayton K, Ferdig MT, , 2009. Probing the multifactorial basis of Plasmodium falciparum quinine resistance: evidence for a strain-specific contribution of the sodium-proton exchanger PfNHE. Mol Biochem Parasitol 165: 122131.[Crossref] [Google Scholar]
  7. Henry M, Briolant S, Zettor A, Pelleau S, Baragatti M, Baret E, Mosnier J, Amalvict R, Fusai T, Rogier C, Pradines B, , 2009. Plasmodium falciparum Na+/H+ exchanger 1 transporter is involved in reduced susceptibility to quinine. Antimicrob Agents Chemother 53: 19261930.[Crossref] [Google Scholar]
  8. Kaddouri H, Nakache S, Houzé S, Mentré F, Le Bras J, , 2006. Assessment of the drug susceptibility of Plasmodium falciparum clinical isolates from Africa by using a Plasmodium lactate dehydrogenase immunodetection assay and an inhibitory maximum effect model for precise measurement of the 50-percent inhibitory concentration. Antimicrob Agents Chemother 50: 33433349.[Crossref] [Google Scholar]
  9. Rason MA, Randriantsoa T, Andrianantenaina H, Ratsimbasoa A, Menard D, , 2008. Performance and reliability of the SYBR Green I-based assay for the routine monitoring of susceptibility of Plasmodium falciparum clinical isolates. Trans R Soc Trop Med Hyg 102: 346351.[Crossref] [Google Scholar]
  10. Rakotonirina H, Barnadas C, Raherijafy R, Andrianantenaina H, Ratsimbasoa A, Randrianasolo L, Jahevitra M, Andriantsoanirina V, Menard D, , 2008. Accuracy and reliability of malaria diagnostic techniques for guiding febrile outpatient treatment in malaria-endemic countries. Am J Trop Med Hyg 78: 217221. [Google Scholar]
  11. Ranford-Cartwright LC, Taylor J, Umasunthar T, Taylor LH, Babiker HA, Lell B, Schmidt-Ott JR, Lehman LG, Walliker D, Kremsner PG, , 1997. Molecular analysis of recrudescent parasites in a Plasmodium falciparum drug efficacy trial in Gabon. Trans R Soc Trop Med Hyg 91: 719724.[Crossref] [Google Scholar]
  12. Durand R, Jafari S, Vauzelle J, Delabre JF, Jesic Z, Le Bras J, , 2001. Analysis of pfcrt mutations and chloroquine susceptibility in isolates of Plasmodium falciparum . Mol Biochem Parasitol 114: 95102.[Crossref] [Google Scholar]
  13. Choudhary V, Sharma YD, , 2009. Extensive heterozygosity in flanking microsatellites of Plasmodium falciparum Na+/H+ exchanger (pfnhe-1) gene among Indian isolates. Acta Trop 109: 241244.[Crossref] [Google Scholar]
  14. Randrianarivelojosia M, Fidock DA, Belmonte O, Valderramos SG, Mercereau-Puijalon O, Ariey F, , 2006. First evidence of pfcrt mutant Plasmodium falciparum in Madagascar. Trans R Soc Trop Med Hyg 100: 826830.[Crossref] [Google Scholar]
  15. Andriantsoanirina V, Andrianaranjaka V, Ratsimbasoa A, Bouchier C, Jahevitra M, Rabearimanana S, Radrianjafy R, Randriantsoa T, Rason MA, Tichit M, Rabarijaona LP, Mercereau-Puijalon O, Durand R, Ménard D, , 2009. Plasmodium falciparum drug resistance in Madagascar: facing the spread of unusual pfdhfr and pfmdr-1 haplotypes and the decrease of the dihydroartemisinin susceptibility. Antimicrob Agents Chemother 53: 45884597.[Crossref] [Google Scholar]
  16. Rason MA, Andrianantenaina HB, Ariey F, Raveloson A, Domarle O, Randrianarivelojosia M, , 2007. Prevalent Pfmdr-1 N86Y mutant Plasmodium falciparum in Madagascar despite absence of Pfcrt mutant strains. Am J Trop Med Hyg 76: 10791083. [Google Scholar]
  17. Vinayak S, Alam MT, Upadhyay M, Das MK, Dev V, Singh N, Dash AP, Sharma YD, , 2007. Extensive genetic diversity in the Plasmodium falciparum Na+/H+ exchanger 1 transporter protein implicated in quinine resistance. Antimicrob Agents Chemother 51: 45084511.[Crossref] [Google Scholar]
  18. Jelinek T, Grobusch MP, Loscher T, , 2001. Patterns of Plasmodium falciparum drug resistance in nonimmune travellers to Africa. Eur J Clin Microbiol Infect Dis 20: 284286. [Google Scholar]
  19. Picot S, Olliaro P, de Monbrison F, Bienvenu AL, Price RN, Ringwald P, , 2009. A systematic review and meta-analysis of evidence for correlation between molecular markers of parasite resistance and treatment outcome in falciparum malaria. Malar J 8: 89.[Crossref] [Google Scholar]

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  • Received : 15 Jun 2009
  • Accepted : 15 Dec 2009
  • Published online : 05 May 2010

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