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Volume 82, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

We sought to test the association of polymorphisms in (, gene PF13_0019) with susceptibility to quinine, which was previously reported in a limited number of reference strains or culture-adapted isolates. Determination of susceptibility to quinine, genotyping of ms4760 microsatellite and polymorphism in codon 76 of were performed for 83 isolates obtained from symptomatic malaria-infected travelers returning from various African countries to France or from subjects living in Madagascar. Nineteen different ms4760 microsatellite profiles of were found including 14 not previously described. Multivariate analysis showed no significant association between the susceptibility to quinine with particular ms4760 profiles. Contrary to previous reports, we only observed that the number of NHNDNHNNDDD repeats was positively associated with the increased IC50 of QN ( = 0.01). We concluded that the studied polymorphisms in did not appear as valid molecular markers of susceptibility to quinine in isolates from Africa. Because we did not include any isolate of Asian origin in our series, these results did not exclude the possibility of regional associations, for example in South-East Asia.

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  • Received : 15 Jun 2009
  • Accepted : 15 Dec 2009

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