Volume 82, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Reemerged malaria in South Korea has not yet been eradicated despite continuous governmental efforts. It has rather become an endemic disease. Our study aimed to determine the genetic diversity in merozoite surface protein-1 (PvMSP-1) and circumsporozoite protein (PvCSP) genes over an extended period after its reemergence to its current status. Sequence analysis of PvMSP-1 gene sequences from the 632 isolates during 1996–2007 indicates that most isolates recently obtained were different from isolates obtained in the initial reemergence period. There was initially only one subtype (recombinant) present but its subtypes have varied since 2000; six MSP-1 subtypes were recently found. A similar variation was observed by CSP gene analysis; a new CSP subtype was found. Understanding genetic variation patterns of the parasite may help to analyze trends and assess extent of endemic malaria in South Korea.


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  1. Mendis K, Sina BJ, Marchesini P, Carter R, , 2001. The neglected burden of Plasmodium vivax malaria. Am J Trop Med Hyg 64: 97106.
  2. Lee HW, Shin EH, Cho SH, Lee HI, Kim CL, Lee WG, Moon SU, Lee JS, Lee WJ, Kim TS, , 2002. Detection of vivax sporozoites naturally infected in Anopheline mosquitoes from endemic areas of northern parts of Gyeonggi-do (province) in Korea. Korean J Parasitol 40: 7581.[Crossref]
  3. Chai JY, , 1999. Re-emerging Plasmodium vivax malaria in the Republic of Korea. Korean J Parasitol 37: 129143.[Crossref]
  4. Chai IH, , 1994. Occurrence of tertian malaria in a male patient who has never been abroad. Korean J Parasitol 32: 195200.[Crossref]
  5. Leclerc MC, Gauthier C, Villegas L, Urdaneta L, , 2005. Genetic diversity of merozoite surface protein-1 gene of Plasmodium vivax isolates in mining villages of Venezuela (Bolivar State). Acta Trop 95: 2632.[Crossref]
  6. Del Portillo HA, Longacre S, Khouri E, David PH, , 1991. Primary structure of the merozoite surface antigen 1 of Plasmodium vivax reveals sequences conserved between different Plasmodium species. Proc Natl Acad Sci USA 88: 40304034.[Crossref]
  7. Putaporntip C, Jongwutiwes S, Tanabe K, Thaithong S, , 1997. Interallelic recombination in the merozoite surface protein-1 (MSP-1) gene of Plasmodium vivax from Thai isolates. Mol Biochem Parasitol 84: 4956.[Crossref]
  8. Zakeri S, Djadid ND, Zeinali S, , 2003. Sequence heterogeneity of the merozoite surface protein-1 gene (MSP-1) of Plasmodium vivax wild isolates in southeastern Iran. Acta Trop 88: 9197.[Crossref]
  9. Lim CS, Kim SH, Kown SI, Song JW, Song KJ, Lee KN, , 2000. Analysis of Plasmodium vivax merosoite surface protein-1 gene sequences from resurgent Korean isolates. Am J Trop Med Hyg 62: 261265.
  10. Arnot DE, Barnwell JW, Tam JP, Nussenzweig V, Nussenzweig RS, Enea V, , 1985. Circumsporozoite protein of Plasmodium vivax: gene cloning and characterization of the immunodominant epitope. Science 230: 815818.[Crossref]
  11. Rosenberg R, Wirtz RA, Lanar DE, Sattabongkot J, Hall T, Waters AP, Prasittisuk C, , 1989. Circumsporozoite protein heterogeneity in the human malaria parasite Plasmodium vivax. Science 245: 973976.[Crossref]
  12. Gonzalez JM, Hurtado S, Arevalo-Herrera M, Herrera S, , 2001. Variants of Plasmodium vivax circumsporozoite protein (VK210 and VK247) in Colombian isolates. Mem Inst Oswaldo Cruz 96: 709712.[Crossref]
  13. Zakeri S, Abouie-Mehrizi A, Djadid ND, Snounou G, , 2006. Circumsporozoite protein gene diversity among temperate and tropical Plasmodium vivax isolates from Iran. Trop Med Int Health 11: 729737.[Crossref]
  14. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG, The Cluster X Windows interface, 1997. Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25: 48764882.[Crossref]
  15. Kumar S, Tamura K, Jakobsen IB, Nei M, , MEGA2, 2001. Molecular Evolutionary Genetics Analysis Software. Tempe, AZ: Arizona State University.
  16. Bonilla JA, Validum L, Cummings R, Palmer CJ, , 2006. Genetic diversity of Plasmodium vivax PvCSP and PvMSP1 in Guyana, South America. Am J Trop Med Hyg 75: 830835.
  17. Lim CS, Kim YK, Lee KN, Kim SH, Hoffman KJ, Song KJ, Song JW, , 2001. The analysis of circumsporozoite protein gene sequences from South Korean isolates of Plasmodium vivax. Ann Trop Med Parasitol 95: 229235.[Crossref]
  18. Kho WG, Chung JY, Sim EJ, Kim DW, Chung WC, , 2001. Analysis of polymorphic regions of Plasmodium vivax Duffy binding protein of Korean isolates. Korean J Parasitol 39: 143150.[Crossref]
  19. Chung JY, Chun EH, Chun JH, Kho WG, , 2003. Analysis of the Plasmodium vivax apical membrane antigen-1 gene from reemerging Korean isolates. Parasitol Res 90: 325329.[Crossref]
  20. Chai JY, Park YK, Guk SM, Oh KH, Oh MD, Lee SH, Kim HS, Wataya Y, , 2000. A trial for a DNA diagnosis of Plasmodium vivax malaria recently reemerging in the Republic of Korea using microtiter plate hybridization assay. Am J Trop Med Hyg 63: 8084.
  21. Kolakovich KA, Ssengoba A, Wojcik K, Tsuboi T, Al-Yaman F, Alpers M, Adams JH, , 1996. Plasmodium vivax: favored gene frequencies of the merozoite surface protein-1 and the multiplicity of infection in a malaria endemic region. Exp Parasitol 83: 1118.[Crossref]
  22. Figtree M, Pasay CJ, Slade R, Cheng Q, Cloonan N, Walker J, Saul A, , 2000. Plasmodium vivax synonymous frequencies, evolution and population structure deduced from diversity in AMA-1 and MSP-1 genes. Mol Biochem Parasitol 108: 5366.[Crossref]
  23. Cui L, Escalante AA, Imwong M, Snounou G, , 2003. The genetic diversity of Plasmodium vivax populations. Trends Parasitol 19: 220226.[Crossref]
  24. Santos-Ciminera PD, Alecrim MGC, Roberts DR, Quinnan GV, Jr, 2007. Molecular epidemiology of Plasmodium vivax in the State of Amazonas, Brazil. Acta Trop 102: 3846.[Crossref]
  25. Amanda M, Sujatha S, Gul A, Asif M, Marcela E, Mauricio C, Silvia B, Virander SC, Pawan M, , 2004. Inter-allelic recombination in the Plasmodium vivax merozoite surface protein 1 gene among Indian and Colombian isolates. Malar J 3: 410.[Crossref]
  26. Moon SU, Lee HW, Kim JY, Na BK, Cho SH, Lin K, Sohn WM, Kim TS, , 2009. High frequency of genetic diversity of Plasmodium vivax field isolates in Myanmar. Acta Trop 109: 3036.[Crossref]
  27. Romi R, Sabatineli G, Majori G, , 2001. Could malaria reappear in Italy? Emerg Infect Dis 7: 915919.[Crossref]
  28. Shin EH, Guk SM, Kim HJ, Lee SH, Chai JY, , 2008. Trends in parasitic diseases in Republic of Korea. Trends Parasitol 24: 143150.[Crossref]

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  • Received : 06 May 2009
  • Accepted : 28 Sep 2009

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