Volume 82, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



The merozoite surface protein 1 (MSP1) is the principal surface antigen of the blood stage form of the parasite. Antibodies recognizing MSP1 are frequently detected following infection, making this protein a significant component of malaria vaccines and diagnostic tests. Although the MSP1 gene sequence has been reported for and , this gene has not been identified for the other two major human-infectious species, and . MSP1 genes from these two species were isolated from Cameroon blood donor samples. The genes are similar in size to known MSP1 genes and encode proteins with interspecies conserved domains homologous to those identified in other species. Sequence and phylogenetic analysis of all available MSP1 amino acid sequences clearly shows that the and MSP1 sequences are truly unique within the genus and not simply or variants.


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  1. Korenromp E, Miller J, Nahlen B, Wardlaw T, Young M, , 2005. World Malaria Report. Available at: http://rbm.who.int/wmr2005/html/toc.htm. [Google Scholar]
  2. Collins WE, Barnwell JW, , 2009. Plasmodium knowlesi: finally being recognized. J Infect Dis 199: 11071108.[Crossref] [Google Scholar]
  3. Lysenko AJ, Beljaev AE, , 1969. An analysis of the geographical distribution of Plasmodium ovale . Bull World Health Organ 40: 383394. [Google Scholar]
  4. Mueller I, Zimmerman PA, Reeder JC, , 2007. Plasmodium malariae and Plasmodium ovale–the “bashful” malaria parasites. Trends Parasitol 23: 278283.[Crossref] [Google Scholar]
  5. Cox-Singh J, Davis TM, Lee KS, Shamsul SS, Matusop A, Ratnam S, Rahman HA, Conway DJ, Singh B, , 2008. Plasmodium knowlesi malaria in humans is widely distributed and potentially life threatening. Clin Infect Dis 46: 165171.[Crossref] [Google Scholar]
  6. White NJ, , 2008. Plasmodium knowlesi: the fifth human malaria parasite. Clin Infect Dis 46: 172173.[Crossref] [Google Scholar]
  7. Florens L, Washburn MP, Raine JD, Anthony RM, Grainger M, Haynes JD, Moch JK, Muster N, Sacci JB, Tabb DL, Witney AA, Wolters D, Wu Y, Gardner MJ, Holder AA, Sinden RE, Yates JR, Carucci DJ, , 2002. A proteomic view of the Plasmodium falciparum life cycle. Nature 419: 520526.[Crossref] [Google Scholar]
  8. Fandeur T, Volney B, Peneau C, de Thoisy B, , 2000. Monkeys of the rainforest in French Guiana are natural reservoirs for P. brasilianum/P. malariae malaria. Parasitology 120:1121.[Crossref] [Google Scholar]
  9. Gerold P, Schofield L, Blackman MJ, Holder AA, Schwarz RT, , 1996. Structural analysis of the glycosyl-phosphatidylinositol membrane anchor of the merozoite surface proteins-1 and -2 of Plasmodium falciparum . Mol Biochem Parasitol 75: 131143.[Crossref] [Google Scholar]
  10. Blackman MJ, , 2000. Proteases involved in erythrocyte invasion by the malaria parasite: function and potential as chemotherapeutic targets. Curr Drug Targets 1: 5983.[Crossref] [Google Scholar]
  11. Blackman MJ, Whittle H, Holder AA, , 1991. Processing of the Plasmodium falciparum major merozoite surface protein-1: identification of a 33-kilodalton secondary processing product which is shed prior to erythrocyte invasion. Mol Biochem Parasitol 49: 3544.[Crossref] [Google Scholar]
  12. O'Dea KP, McKean PG, Harris A, Brown KN, , 1995. Processing of the Plasmodium chabaudi chabaudi AS merozoite surface protein 1 in vivo and in vitro . Mol Biochem Parasitol 72:111119.[Crossref] [Google Scholar]
  13. Blackman MJ, Dennis ED, Hirst EM, Kocken CH, Scott-Finnigan TJ, Thomas AW, , 1996. Plasmodium knowlesi: secondary processing of the malaria merozoite surface protein-1. Exp Parasitol 83: 229239.[Crossref] [Google Scholar]
  14. Tolle R, Fruh K, Doumbo O, Koita O, N'Diaye M, Fischer A, Dietz K, Bujard H, , 1993. A prospective study of the association between the human humoral immune response to Plasmodium falciparum blood stage antigen gp190 and control of malarial infections. Infect Immun 61: 4047. [Google Scholar]
  15. Egan AF, Chappel JA, Burghaus PA, Morris JS, McBride JS, Holder AA, Kaslow DC, Riley EM, , 1995. Serum antibodies from malaria-exposed people recognize conserved epitopes formed by the two epidermal growth factor motifs of MSP1(19), the carboxy-terminal fragment of the major merozoite surface protein of Plasmodium falciparum . Infect Immun 63: 456466. [Google Scholar]
  16. Faber BW, Remarque EJ, Morgan WD, Kocken CH, Holder AA, Thomas AW, , 2007. Malaria vaccine-related benefits of a single protein comprising Plasmodium falciparum apical membrane antigen 1 domains I and II fused to a modified form of the 19-kilodalton C-terminal fragment of merozoite surface protein 1. Infect Immun 75: 59475955.[Crossref] [Google Scholar]
  17. Stowers AW, Cioce V, Shimp RL, Lawson M, Hui G, Muratova O, Kaslow DC, Robinson R, Long CA, Miller LH, , 2001. Efficacy of two alternate vaccines based on Plasmodium falciparum merozoite surface protein 1 in an Aotus challenge trial. Infect Immun 69: 15361546.[Crossref] [Google Scholar]
  18. Kim S, Ahn HJ, Kim TS, Nam HW, , 2003. ELISA detection of vivax malaria with recombinant multiple stage-specific antigens and its application to survey of residents in endemic areas. Korean J Parasitol 41: 203207.[Crossref] [Google Scholar]
  19. Kitchen AD, Lowe PH, Lalloo K, Chiodini PL, , 2004. Evaluation of a malarial antibody assay for use in the screening of blood and tissue products for clinical use. Vox Sang 87: 150155.[Crossref] [Google Scholar]
  20. Walker-Abbey A, Djokam RR, Eno A, Leke RF, Titanji VP, Fogako J, Sama G, Thuita LH, Beardslee E, Snounou G, Zhou A, Taylor DW, , 2005. Malaria in pregnant Cameroonian women: the effect of age and gravidity on submicroscopic and mixed-species infections and multiple parasite genotypes. Am J Trop Med Hyg 72: 229235. [Google Scholar]
  21. Mangold KA, Manson RU, Koay ES, Stephens L, Regner M, Thomson RB, Jr Peterson LR, Kaul KL, , 2005. Real-time PCR for detection and identification of Plasmodium spp. J Clin Microbiol 43: 24352440.[Crossref] [Google Scholar]
  22. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ, , 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25: 33893402.[Crossref] [Google Scholar]
  23. Sambrook J, Russell DW, , 2001. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press. [Google Scholar]
  24. Genetics Computer Group, 2005. Wisconsin Package Version 11.0. San Diego, CA: Accelrys Inc. [Google Scholar]
  25. Tamura K, Dudley J, Nei M, Kumar S, , 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24: 15961599.[Crossref] [Google Scholar]
  26. Nei M, Gojobori T, , 1986. Simple methods for estimating the numbers of synonymous and nonsynonymous nucleotide substitutions. Mol Biol Evol 3: 418426. [Google Scholar]
  27. Eisenhaber B, Bork P, Eisenhaber F, , 1999. Prediction of potential GPI-modification sites in proprotein sequences. J Mol Biol 292: 741758.[Crossref] [Google Scholar]
  28. Bendtsen JD, Nielsen H, von Heijne G, Brunak S, , 2004. Improved prediction of signal peptides: SignalP 3.0. J Mol Biol 340: 783795.[Crossref] [Google Scholar]
  29. Jones DT, Taylor WR, Thornton JM, , 1992. The rapid generation of mutation data matrices from protein sequences. Comput Appl Biosci 8: 275282. [Google Scholar]
  30. Felsenstein J, , 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783791.[Crossref] [Google Scholar]
  31. Win TT, Jalloh A, Tantular IS, Tsuboi T, Ferreira MU, Kimura M, Kawamoto F, , 2004. Molecular analysis of Plasmodium ovale variants. Emerg Infect Dis 10: 12351240.[Crossref] [Google Scholar]
  32. Tanabe K, Escalante A, Sakihama N, Honda M, Arisue N, Horii T, Culleton R, Hayakawa T, Hashimoto T, Longacre S, Pathirana S, Handunnetti S, Kishino H, , 2007. Recent independent evolution of msp1 polymorphism in Plasmodium vivax and related simian malaria parasites. Mol Biochem Parasitol 156: 7479.[Crossref] [Google Scholar]
  33. Blackman MJ, Ling IT, Nicholls SC, Holder AA, , 1991. Proteolytic processing of the Plasmodium falciparum merozoite surface protein-1 produces a membrane-bound fragment containing two epidermal growth factor-like domains. Mol Biochem Parasitol 49: 2933.[Crossref] [Google Scholar]
  34. Deleersnijder W, Hendrix D, Bendahman N, Hanegreefs J, Brijs L, Hamers-Casterman C, Hamers R, , 1990. Molecular cloning and sequence analysis of the gene encoding the major merozoite surface antigen of Plasmodium chabaudi chabaudi IP-PC1. Mol Biochem Parasitol 43: 231244.[Crossref] [Google Scholar]
  35. Zhong H, Fan JY, Yang S, Davidson EA, , 1999. Cloning and characterization of the merozoite surface antigen 1 gene of Plasmodium berghei . Am J Trop Med Hyg 60: 994999. [Google Scholar]
  36. Koussis K, Withers-Martinez C, Yeoh S, Child M, Hackett F, Knuepfer E, Juliano L, Woehlbier U, Bujard H, Blackman MJ, , 2009. A multifunctional serine protease primes the malaria parasite for red blood cell invasion. EMBO J 28: 725735.[Crossref] [Google Scholar]
  37. Withers-Martinez C, Jean L, Blackman MJ, , 2004. Subtilisin-like proteases of the malaria parasite. Mol Microbiol 53: 5563.[Crossref] [Google Scholar]
  38. Harris PK, Yeoh S, Dluzewski AR, O'Donnell RA, Withers-Martinez C, Hackett F, Bannister LH, Mitchell GH, Blackman MJ, , 2005. Molecular identification of a malaria merozoite surface sheddase. PLoS Pathog 1: 241251.[Crossref] [Google Scholar]
  39. Naik RS, Branch OH, Woods AS, Vijaykumar M, Perkins DJ, Nahlen BL, Lal AA, Cotter RJ, Costello CE, Ockenhouse CF, Davidson EA, Gowda DC, , 2000. Glycosylphosphatidylinositol anchors of Plasmodium falciparum: molecular characterization and naturally elicited antibody response that may provide immunity to malaria pathogenesis. J Exp Med 192: 15631576.[Crossref] [Google Scholar]
  40. Udenfriend S, Kodukula K, , 1995. How glycosylphosphatidylinositol-anchored membrane proteins are made. Annu Rev Biochem 64: 563591.[Crossref] [Google Scholar]
  41. Hughes AL, , 1992. Positive selection and interallelic recombination at the merozoite surface antigen-1 (MSA-1) locus of Plasmodium falciparum . Mol Biol Evol 9: 381393. [Google Scholar]
  42. Leclerc MC, Hugot JP, Durand P, Renaud F, , 2004. Evolutionary relationships between 15 Plasmodium species from new and old world primates (including humans): an 18S rDNA cladistic analysis. Parasitology 129: 677684.[Crossref] [Google Scholar]
  43. Ollomo B, Durand P, Prugnolle F, Douzery E, Arnathau C, Nkoghe D, Leroy E, Renaud F, , 2009. A new malaria agent in African hominids. PLoS Pathog 5: e1000446.[Crossref] [Google Scholar]
  44. Perkins SL, Schall JJ, , 2002. A molecular phylogeny of malarial parasites recovered from cytochrome b gene sequences. J Parasitol 88: 972978.[Crossref] [Google Scholar]
  45. Rich SM, Leendertz FH, Xu G, LeBreton M, Djoko CF, Aminake MN, Takang EE, Diffo JL, Pike BL, Rosenthal BM, Formenty P, Boesch C, Ayala FJ, Wolfe ND, , 2009. The origin of malignant malaria. Proc Natl Acad Sci USA 106: 1490214907.[Crossref] [Google Scholar]
  46. Van de Peer Y, Baldauf SL, Doolittle WF, Meyer A, , 2000. An updated and comprehensive rRNA phylogeny of (crown) eukaryotes based on rate-calibrated evolutionary distances. J Mol Evol 51: 565576.[Crossref] [Google Scholar]

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  • Received : 13 Jan 2009
  • Accepted : 02 Mar 2010
  • Published online : 04 Jun 2010

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