Volume 81, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


The major malaria vector belongs to a group of morphologically similar species that are commonly distinguished from one another through the use of chromosomal and molecular techniques. Indoor resting collections of mosquitoes from Malawi were initially identified as by morphology, but failed to have this confirmed by the species-specific polymerase chain reaction assay. Sequence analysis of the internal transcribed spacer region 2 identified variations within the -specific primer binding site and showed a sequence variation of 4.5% compared with . Domain 3 analysis showed sequence variation of 1.5% from . Cytogenetic analysis of the polytene chromosome banding arrangements showed that the specimens were homosequential with , with fixed inverted arrangements of the 3a, 3b, and 5a inversions commonly polymorphic in . The chromosomes of hybrid females showed levels of asynapsis typical of inter-species crosses. These molecular and cytogenetic observations support the conclusion that this Malawi population is a new species and it has provisionally been named -like.


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  • Received : 06 Nov 2008
  • Accepted : 28 Apr 2009

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