1921
Volume 81, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

causes human neurocysticercosis and is endemic in underdeveloped countries where backyard pig keeping is common. Microscopic fecal diagnostic methods for human taeniasis are not very sensitive, and and eggs are indistinguishable under the light microscope. Coproantigen (CoAg) ELISA methods are very sensitive, but currently only genus () specific. This paper describes the development of a highly species-specific coproantigen ELISA test to detect intestinal taeniasis. Sensitivity was maintained using a capture antibody of rabbit IgG against adult whole worm somatic extract, whereas species specificity was achieved by utilization of an enzyme-conjugated rabbit IgG against adult excretory-secretory (ES) antigen. A known panel of positive and negative human fecal samples was tested with this hybrid sandwich ELISA. The ELISA test gave 100% specificity and 96.4% sensitivity for tapeworm carriers ( = 28), with a index of 0.96. This simple ELISA incorporating anti-adult somatic and anti-adult ES antibodies provides the first potentially species-specific coproantigen test for human taeniasis.

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2009-09-01
2017-11-19
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References

  1. Garcia HH, Martinez M, Gilman RH, Herrera G, Tsang VCW, Pilcher JB, Diaz F, Verastegui M, Gallo C, Porras M, Alvarado, M, Naranjo J, Miranda E, and the Cysticercosis Working Groupin Perú, 1991. Diagnosis of cysticercosis in endemic regions. Lancet 338 : 549–551.
  2. Schantz PM, Sarti E, 1989. Diagnostic methods and epidemiological surveillance of Taenia solium infection. Acta Leiden 57 : 153–163.
  3. Mayta H, Talley A, Gilman RH, Jimenez J, Verastegui M, Ruiz M, Garcia HH, Gonzalez AE, 2000. Differentiating Taenia solium and Taenia saginata infections by simple hematoxylin-eosin staining and PCR-restriction enzyme analysis. J Clin Microbiol 38 : 133–137.
  4. Pawlowski ZS, Allan JC, Meinardi H, 2005. Control measures for taeniosis and cysticercosis. Murrell KD, ed. WHO/FAO/OIE Guidelines for the Surveillance, Prevention and Control of Taeniosis/Cysticercosis. Paris: OIE, 73–92.
  5. Allan JC, Craig PS, 2006. Coproantigens in taeniasis and echinococcosis. Parasitol Int 55 : S75–S80.
  6. Allan JC, Craig PS, 1989. Coproantigens in gut tapeworms infections: Hymenolepis diminuta in rats. Parasitol Res 76 : 68–73.
  7. Allan JC, Avila G, Garcia Noval J, Flisser A, Craig PS, 1990. Immunodiagnosis of taeniasis by coproantigens detection. Parasitology 101 : 473–477.
  8. Allan JC, Wilkins P, Tsang VWC, Craig PS, 2003. Immunodiagnostic tools for taeniasis. Acta Trop 87 : 87–93.
  9. Ito A, Craig P, 2003. Immunodiagnostic and molecular approaches for the detection of taeniid cestode infections. Trends Parasitol 19 : 377–381.
  10. Wandra T, Margono SS, Gafar MS, Saragih JM, Sutisna P, Sudewi AA, Depary AA, Yulfi H, Darlan DM, Okamoto M, Sato MO, Sako Y, Nakao M, Nakaya K, Craig PS, Ito A, 2007. Current situation of taeniasis and cysticercosis in Indonesia. Trop Med Health 35 : 323–328.
  11. Machnicka B, Dziemian E, Zwierz C, 1996. Factors conditioning detection of Taenia saginata antigens in feces. Appl Parasitol 37 : 99–105.
  12. Allan JC, Mencos F, Garcia Noval J, Sarti E, Flisser A, Wang Y, Liu D, Craig PS, 1993. Dipstick dot ELISA for the detection of coproantigens in humans. Parasitology 107 : 79–85.
  13. Allan JC, Velasquez-Tohom M, Torres-Alvarez R, Yurrita P, Garcia-Noval J, 1996a. Field trial of the coproantigen-based diagnosis of Taenia solium taeniasis by enzyme-linked immunosorbent assay. Am J Trop Med Hyg 54 : 352–356.
  14. Maravilla P, Avila G, Cabrera V, Aguilar L, Flisser A, 1998. Comparative development of Taenia solium in experimental models. J Parasitol 84 : 882–886.
  15. Avila G, Benitez M, Aguilar-Vega L, Flisser A, 2003. Kinetics of Taenia solium antibodies and antigens in experimental taeniosis. Parasitol Res 89 : 284–289.
  16. Fraser A, Craig PS, 1997. Detection of gastrointestinal helminthic infections using coproantigens and molecular approaches. J Helminthol 71 : 103–107.
  17. Deplazes P, Gottstein B, Stingelin Y, Eckert J, 1990. Detection of Taenia hydatigena coproantigens by ELISA in dogs. Vet Parasitol 36 : 91–103.
  18. Deplazes P, Gottstein B, Eckert J, Jenkins DJ, Ewald D, Jimenez-Palacios S, 1992. Detection of Echinococcus coproantigens by enzyme-linked immunosorbent assay in dogs, dingoes and foxes. Parasitol Res 78 : 303–308.
  19. Jeri C, Gilman RH, Lescano AG, Mayta H, Ramirez ME, Gonzalez AE, Nazerali R, Garcia HH, 2004. Species identification after treatment for human taeniasis. Lancet 363 : 949–959.
  20. Yamasaki H, Allan JC, Sato MO, Nakao M, Sako Y, Nakaya K, Qiu D, Mamuti W, Craig PS, Ito A, 2004. DNA differential diagnosis of taeniasis and cysticercosis by multiplex PCR. J Clin Microbiol 42 : 548–553.
  21. Wilkins PP, Allan JC, Verastegui M, Acosta M, Eason AG, Garcia HH, Gonzalez AE, Gilman RH, Tsang VWC,1999. Development of a serologic assay to detect Taenia solium taeniasis. Am J Trop Med Hyg 60 : 199–204.
  22. Levine MZ, Calderon JC, Wilkins PP, Lane WS, Asara JM, Hancock K, Gonzalez AE, Garcia HH, Gilman RH, Tsang VCW, 2004. Characterization, cloning, and expression of two diagnostic antigens for Taenia solium tapeworm infection. J Parasitol 90 : 631–638.
  23. Wilson MB, Nakane PK, 1978. Recent developments in the periodate method of conjugating horseradish peroxidase (HRPO) to antibodies. Knapp W, Holubar K, Wicks G, eds. Immunofluorescence and Related Staining Techniques. Amsterdam: Elsevier, 215–224.
  24. Bradford MM, 1976. A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem 72 : 248–254.
  25. Youden WJ, 1950. Index for rating diagnostic tests. Cancer 3 : 32–35.
  26. Allan JC, Velasquez-Tohom M, Garcia-Noval J, Torres-Alvarez R, Yurrita P, Fletes C, de Mata F, Soto de Alfaro H, Craig PS, 1996. Epidemiology of intestinal taeniasis in four rural Guatemalan communities. Ann Trop Med Parasitol 90 : 157–165.
  27. Elayoubi FA, Craig PS, 2004. Echinococcus granulosus coproantigens: chromatographic fractionation and characterization. Parasitology 128 : 1–11.
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  • Received : 18 Aug 2008
  • Accepted : 23 May 2009

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