Volume 81, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


causes human neurocysticercosis and is endemic in underdeveloped countries where backyard pig keeping is common. Microscopic fecal diagnostic methods for human taeniasis are not very sensitive, and and eggs are indistinguishable under the light microscope. Coproantigen (CoAg) ELISA methods are very sensitive, but currently only genus () specific. This paper describes the development of a highly species-specific coproantigen ELISA test to detect intestinal taeniasis. Sensitivity was maintained using a capture antibody of rabbit IgG against adult whole worm somatic extract, whereas species specificity was achieved by utilization of an enzyme-conjugated rabbit IgG against adult excretory-secretory (ES) antigen. A known panel of positive and negative human fecal samples was tested with this hybrid sandwich ELISA. The ELISA test gave 100% specificity and 96.4% sensitivity for tapeworm carriers ( = 28), with a index of 0.96. This simple ELISA incorporating anti-adult somatic and anti-adult ES antibodies provides the first potentially species-specific coproantigen test for human taeniasis.


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  • Received : 18 Aug 2008
  • Accepted : 23 May 2009

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