Volume 81, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Alterations in gene expression in the midgut of female exposed to blood meals containing 6.8 logs plaque-forming units/mL of West Nile virus (WNV) were studied by fluorescent differential display. Twenty-six different cDNAs exhibited reproducible differences after feeding on infected blood. Of these, 21 cDNAs showed an increase in expression, and 5 showed a decrease in expression as a result of WNV presence in the blood meal. GenBank database searches showed that one clone with increased expression, CQ G12A2, shares 94% identity with a leucine-rich repeat-containing protein from and 32% identity to Toll-like receptors from . We present the first cDNA clone isolated from female midgut tissue whose expression changes on exposure to WNV. This cDNA represents a mosquito gene that is an excellent candidate for interacting with WNV in and may play a role in disease transmission.


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  • Received : 10 Mar 2009
  • Accepted : 12 May 2009

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