1921
Volume 81, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

This study describes conventional and real-time polymerase chain reaction (PCR) methods developed to detect and quantify DNA in cynomolgus monkeys () using formalin-fixed paraffin-embedded blocks archived for periods of up to 6 years. The highest concentration of DNA was found in the myocardium, urinary bladder, stomach, lymph node, adrenal gland, and colon. The concentration of DNA detected in cardiac tissues was 10–100-fold greater than found elsewhere; the mean concentrations of DNA in non-cardiac tissues were otherwise comparable. DNA was amplified from cerebrum but not cerebellum or kidney. Successful use of DNA from formalin-fixed, paraffin-embedded blocks is important because most pathology laboratories routinely archive wax blocks. This archived resource can be used for further studies on the prevalence of this disease.

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2009-08-01
2017-09-19
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  • Received : 12 Jan 2009
  • Accepted : 07 May 2009

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