Volume 81, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Cutaneous leishmaniasis (CL) is prevalent in the Egyptian Sinai Peninsula and previous research has consistently documented the etiologic agent to be . We report the first isolation of from human cases of CL in a Northern Sinai community bordering Palestine. Parasite culturing, real-time polymerase chain reaction (PCR), gene sequencing, and restriction fragment length polymorphism (RFLP) analyses indicate CL cases in this community were caused by either or (three cases each). Two wild-caught rodents () were infected with sand flies were found harboring , however only non-infected individuals of , a vector for were caught. Patients with had not traveled from the region in over a year, suggesting these cases are autochthonous. This scenario is consistent with an incursion of from bordering countries and raises concerns about expansion of this parasite further into Egypt.


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  1. Porrozzi R, Teva A, Amaral VF, Santos da Costa MV, Grimaldi G Jr, 2004. Cross-immunity experiments between different species or strains of Leishmania in Rhesus Macaques (Macaca mulatta). Am J Trop Med Hyg 71 : 297–305. [Google Scholar]
  2. Magill AJ, Grogl M, Gasser RA Jr, Sun W, Oster CN, 1993. Visceral infection caused by Leishmania tropica in veterans of operation Desert Storm. N Engl J Med 328 : 1383–1387. [Google Scholar]
  3. Alborzi A, Rasouli M, Shamsizadeh A, 2006. Leishmania tropica-isolated patient with visceral leishmaniasis in southern Iran. Am J Trop Med Hyg 4 : 306–307. [Google Scholar]
  4. Mansour NS, Fryauff DJ, Modi GB, Mikhail EM, Youssef FG, 1991. Isolation and characterization of Leishmania major from Phlebotomus papatasi and military personnel in north Sinai, Egypt. Trans R Soc Trop Med Hyg 85 : 590–591. [Google Scholar]
  5. Dawoud HA, 2004. Identification of cutaneous leishmaniasis in Egypt by hybridization of PCR amplified mini-exon repeats. J Egypt Soc Parasitol 34 : 881–892. [Google Scholar]
  6. Kamal HA, Doha SA, El-Hossary SS, Shehata MG, El Sawaf BM, 2003. Human zoonotic cutaneous leishmaniasis and associated sand flies (Diptera: Psychodidae) in Sheikh Atiya village, southern Sinai, Egypt. J Egypt Soc Parasitol 33 : 795–803. [Google Scholar]
  7. Morsy TA, Salama MM, Saleh MS, 1991. Leishmania major and Meriones crassus in North Sinai Governorate, Egypt. J Egypt Soc Parasitol 21 : 337–342. [Google Scholar]
  8. Morsy TA, Shoukry A, Schnur LF, Sulitzeanu A, 1987. Gerbillus pyramidum is a host of Leishmania major in the Sinai Peninsula. Ann Trop Med Parasitol 81 : 741–742. [Google Scholar]
  9. Fryauff DJ, Modi GB, Mansour NS, Kreutzer RD, Soliman S, Youssef FG, 1993. Epidemiology of cutaneous leishmaniasis at a focus monitored by the multinational force and observers in the northeastern Sinai Desert of Egypt. Am J Trop Med Hyg 49 : 598–607. [Google Scholar]
  10. Schlein Y, Warburg A, Schnur LF, Le Blancq SM, Gunders AE, 1984. Leishmaniasis in Israel: reservoir hosts, sandfly vectors and Leishmania l strains in the Negev, Central Arava and along the Dead Sea. Trans R Soc Trop Med Hyg 78 : 480–484. [Google Scholar]
  11. Parvizi P, Benlarbi M, Ready PD, 2003. Mitochondrial and Wolbachia markers for the sandfly Phlebotomus papatasi: little population differentiation between peridomestic sites and gerbil burrows in Isfahan province, Iran. Med Vet Entomol 17 : 351–362. [Google Scholar]
  12. Jacobson RL, 2003. Leishmania tropica (Kinetoplastida: Trypanosomatidae)–a perplexing parasite. Folia Parasitol (Praha) 50 : 241–250. [Google Scholar]
  13. Garifallou A, Schnur LF, Stratigos JD, Hadziandoniou M, Savigos M, Stavrianeas N, Sérié C, 1984. Leishmaniasis in Greece II. Isolation and identification of the parasite causing cutaneous leishmaniasis in man. Ann Trop Med Parasitol 78 : 369–675. [Google Scholar]
  14. Mebrahtu YB, Lawyer PG, Ngumbi PM, Kirigi G, Mbugua J, Gachihi G, Wasunna K, Pamba H, Sherwood JA, Koech DK, 1992. A new rural focus of cutaneous leishmaniasis caused by Leishmania tropica in Kenya. Trans R Soc Trop Med Hyg 86 : 381–387. [Google Scholar]
  15. Jacobson RL, Eisenberger CL, Svobodova M, Baneth G, Sztern J, Carvalho J, Nasereddin A, El Fari M, Shalom U, Volf P, Votypka J, Dedet JP, Pratlong F, Schonian G, Schnur LF, Jaffe CL, Warburg A, 2003. Outbreak of cutaneous leishmaniasis in northern Israel. J Infect Dis 188 : 1065–1073. [Google Scholar]
  16. Sang DK, Njeru WK, Ashford RW, 1992. A possible animal reservoir for Leishmania tropica s.l. in Kenya. Ann Trop Med Parasitol 86 : 311–312. [Google Scholar]
  17. Svobodova M, Votypka J, Peckova J, Dvorak V, Nasereddin A, Baneth G, Sztern J, Kravchenko V, Orr A, Meir D, Schnur LF, Volf P, Warburg A, 2006. Distinct transmission cycles of Leishmania tropica in 2 adjacent foci, Northern Israel. Emerg Infect Dis 12 : 1860–1868. [Google Scholar]
  18. Seccombe AK, Ready PD, Huddleston LM, 1993. A catalogue of Old World phlebotomine sandflies (Diptera: Psychodidae, Phlebotominae). Occas Pap Syst Entomol 8 : 1–57. [Google Scholar]
  19. Cahill KM, 1965. Leishmania skin testing in Africa and the Middle East. East Afr Med J 42 : 213–220. [Google Scholar]
  20. Mohareb EW, Mikhail EM, Youssef FG, 1996. Leishmania tropica in Egypt: an undesirable import. Trop Med Int Health 1 : 251–254. [Google Scholar]
  21. Lane RP, 1986. The sand flies of Egypt (Diptera: Phlebotominae). Bull Br Mus Nat Hist Entomol 52 : 1–35. [Google Scholar]
  22. Osborn DJ, Helmy I, 1980. Mammals of Egypt. Chicago: Field Museum of Natural History, 1–579.
  23. CIOMS, 1985. International Guiding Principles for Biomedical Research Involving Animals. Altern Lab Anim 12 : ii. [Google Scholar]
  24. Villinski JT, Klena JD, Abbassy MM, Hoel DF, Puplampu N, Mechta S, Boakye D, Raczniak G, 2008. Evidence for a new species of Leishmania associated with a focal disease outbreak in Ghana. Diagn Microbiol Infect Dis 60 : 323–327. [Google Scholar]
  25. Wortmann G, Sweeney C, Houng H-S, Aronson N, Stiteler J, Jackson J, Ockenhouse C, 2001. Rapid diagnosis of leishmaniasis by fluorogenic polymerase chain reaction. Am J Trop Med Hyg 65 : 583–587. [Google Scholar]
  26. Mary C, Faraut F, Lascombe L, Dumon H, 2004. Quantification of Leishmania infantum DNA by real-time PCR assay with high sensitivity. J Clin Microbiol 42 : 5249–5255. [Google Scholar]
  27. Wortmann G, Hochberg L, Houng H-H, Sweeney C, Zapor M, Aronson N, Weina P, Ockenhouse CF, 2005. Rapid identification of Leishmania complexes by a real-time PCR assay. Am J Trop Med Hyg 73 : 999–1004. [Google Scholar]
  28. El Tai NO, Osman OF, El Fari M, Presber W, Schönian G, 2000. Genetic heterogeneity of ribosomal internal transcribed spacer (ITS) in clinical samples of Leishmania donovani spotted on filter paper as revealed by single-strand conformation polymorphisms (sscp) and sequencing. Trans R Soc Trop Med Hyg 94 : 1–5. [Google Scholar]
  29. Hall TA, 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41 : 95–98. [Google Scholar]
  30. Kumar S, Tamura K, Nei M, 2004. MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5 : 150–163. [Google Scholar]
  31. Schönian G, Nasereddin A, Dinse N, Schweynoch C, Schallig HDFH, Presber W, Jaffe CL, 2003. PCR diagnosis and characterization of Leishmania in local and imported clinical samples. Diagn Microbiol Infect Dis 47 : 349–358. [Google Scholar]
  32. Al-Jawabreh A, Schnur LF, Nasereddin A, Schwenkenbecher JM, Abdeen Z, Barghuthy F, Khanfar H, Presber W, Schonian G, 2004. The recent emergence of Leishmania tropica in Jericho (A’riha) and its environs, a classical focus of L. major. rop Med Int Health 9 : 812–816. [Google Scholar]
  33. Schnur LF, Nasereddin A, Eisenberger CL, Jaffe CL, El Fari M, Azmi K, Anders G, Killick-Kendrick M, Killick-Kendrick R, Dedet JP, Pratlong F, Kanaan M, Grossman T, Jacobson RL, Schonian G, Warburg A, 2004. Multifarious characterization of Leishmania tropica from a Judean desert focus, exposing intraspecific diversity and incriminating Phlebotomus sergenti as its vector. Am J Trop Med Hyg 70 : 364–372. [Google Scholar]
  34. Nasereddin A, Bensoussan-Hermano E, Schonian G, Baneth G, Jaffe CL, 2008. Molecular diagnosis of Old World cutaneous leishmaniasis and species identification by use of a reverse line blot hybridization assay. J Clin Microbiol 46 : 2848–2855. [Google Scholar]
  35. Schonian G, Schnur LF, El Fari M, Oskam L, Kolesnikov AA, Sokolowska-Kohler W, Presber W, 2001b. Genetic heterogeneity in the species Leishmania tropica revealed by PCR-based methods. Trans R Soc Trop Med Hyg 95 : 217–224. [Google Scholar]
  36. Desjeux P, 2001. The increase in risk factors for leishmaniasis worldwide. Trans R Soc Trop Med Hyg 95 : 239–243. [Google Scholar]
  37. Morsy TA, El-Missiry AG, Kamal AM, Fayad ME, El-Sharkawy IM, 1990. Distribution of Phlebotomus species in the Nile delta, Egypt. J Egypt Soc Parasitol 20 : 589–598. [Google Scholar]
  38. Hanafi HA, el Sawaf BM, Fryauff DJ, Beavers GM, Tetreault GE, 1998. Susceptibility to Leishmania major of different populations of Phlebotomus papatasi (Diptera: Psychodidae) from endemic and non-endemic regions of Egypt. Ann Trop Med Parasitol 92 : 57–64. [Google Scholar]

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  • Received : 25 Jan 2009
  • Accepted : 31 Mar 2009

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