1921
Volume 81, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

With the implementation of mass drug administration programs for the control of human hookworms, there is a need to monitor for the emergence of drug resistance. We have therefore examined assays for monitoring sensitivity to benzimidazoles (egg hatch assay) and nicotinic acetylcholine receptor agonist drugs (motility and morphology assays), with a view to developing tools for monitoring drug sensitivity in the field. We have performed assays with and , and combined this with published data on and , to indicate the breadth of the responses of various hookworm species and isolates in these assays. This has allowed us to generate assay templates covering the known range of responses, with scope to cover any shift in response that may be indicative of resistance. These assays will have immediate applicability in monitoring for the emergence of drug resistance in human hookworm populations.

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2009-07-01
2017-11-19
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References

  1. Bethony J, Brooker S, Albonico M, Geiger SM, Loukas A, Diemert D, Hotez PJ, 2006. Soil-transmitted helminth infections: ascariasis, trichuriasis, and hookworm. Lancet 367 : 1521–1532.
  2. World Health Organization, 2000. The use of essential drugs. WHO Technical Report Series 895, Geneva.
  3. Kaplan RM, 2004. Drug resistance in nematodes of veterinary importance: a status report. Trends Parasitol 20 : 477–481.
  4. Coles GC, Bauer C, Borgsteede FHM, Geerts S, Klei TR, Taylor MA, Waller PJ, 1992. World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) methods for the detection of anthelmintic resistance in nematodes of veterinary importance. Vet Parasitol 44 : 35–44.
  5. Taylor MA, Hunt KR, Goodyear KL, 2002. Anthelmintic resistance detection methods. Vet Parasitol 103 : 183–194.
  6. Coles GC, Jackson F, Pomroy WE, Prichard RK, von Samson-Himmelstjerna G, Silvestre A, Taylor MA, Vercruysse J, 2006. The detection of anthelmintic resistance in nematodes of veterinary importance. Vet Parasitol 136 : 167–185.
  7. Bennett A, Guyatt H, 2000. Reducing intestinal nematode infection: efficacy of albendazole and mebendazole. Parasitol Today 16 : 71–74.
  8. Kotze AC, Kopp SR, 2008. The potential impact of density dependent fecundity on the use of the Faecal Egg Count Reduction Test for detecting drug resistance in human hookworms. PLoS Negl Trop Dis 2 : e297.
  9. Walsh TK, Donnan AA, Jackson F, Skuce P, Wolstenholme AJ, 2007. Detection and measurement of benzimidazole resistance alleles in Haemonchus contortus using real-time PCR with locked nucleic acid Taqman probes. Vet Parasitol 144 : 304–312.
  10. Kotze AC, Stein PA, Dobson RJ, 1999. Investigation of intestinal nematode responses to naphthalophos and pyrantel using a larval development assay. Int J Parasitol 29 : 1093–1099.
  11. Kopp SR, Coleman GT, McCarthy JS, Kotze AC, 2008a. Application of in vitro anthelmintic sensitivity assays to canine parasitology: detecting resistance to pyrantel in Ancylostoma caninum. Vet Parasitol 152 : 284–293.
  12. Kopp SR, Coleman GT, Traub RJ, McCarthy JS, Kotze AC, 2008. Acetylcholine receptor subunit genes from Ancylostoma caninum: altered transcription patterns associated with pyrantel resistance. Int J Parasitol 39 : 435–441.
  13. Schwenkenbecher JM, Albonico M, Bickle Q, Kaplan RM, 2007. Characterization of beta-tubulin genes in hookworms and investigation of resistance-associated mutations using real-time PCR. Mol Biochem Parasitol 156 : 167–174.
  14. Albonico M, Wright V, Ramsan M, Haji HJ, Taylor M, Savioli L, Bickle Q, 2005. Development of the egg hatch assay for detection of anthelminthic resistance in human hookworms. Int J Parasitol 35 : 803–811.
  15. Kotze AC, Coleman G, Mai A, McCarthy JS, 2005. Field evaluation of anthelmintic drug sensitivity using egg-hatch and larval motility assays with Necator americanus recovered from human clinical isolates. Int J Parasitol 35 : 445–453.
  16. Lacey E, Redwin JM, Gill JH, DeMargheriti VM, Waller PJ, 1990. A larval development assay for simultaneous detection of broad spectrum anthelmintic resistance. Boray, JC, Martin, PJ, Roush, RT eds. Resistance of Parasites to Antiparasitic Drugs. Rahway, NJ: MSD AGVET, 177–184.
  17. Albers GA, Burgess SK, 1988. Serial passage of Haemonchus contortus in resistant and susceptible sheep. Vet Parasitol 28 : 303–306.
  18. Love SCJ, Neilson FJA, Biddle AJ, McKinnon R, 2003. Moxidectin-resistant Haemonchus contortus in sheep in northern New South Wales. Aust Vet J 81 : 359–360.
  19. Le Jambre LF, Geoghegan J, Lyndal-Murphy M, 2005. Characterization of moxidectin resistant Trichostrongylus colubriformis and Haemonchus contortus. Vet Parasitol 128 : 83–90.
  20. Harada Y, Mori O, 1955. A new method for culturing hookworm. Yonago Acta Med 1 : 177–179.
  21. Kotze AC, Clifford S, O’Grady J, Behnke JM, McCarthy JS, 2004. An in vitro larval motility assay to determine anthelmintic sensitivity for human hookworm and Strongyloides species. Am J Trop Med Hyg 71 : 608–616.
  22. Horton J, 2000. Albendazole: a review of anthelmintic efficacy and safety in humans. Parasitology 121 : S113–S132.
  23. Coles GC, East JM, Jenkins SN, 1975. The mechanism of action of the anthelmintic levamisole. Gen Pharmacol 6 : 309–313.
  24. Martin PJ, Le Jambre LF, 1979. Larval paralysis as an in vitro assay of levamisole and morantel tartrate resistance in Ostertagia. Vet Sc Commun 3 : 159–164.
  25. Behnke JM, Rose R, Garside P, 1993. Sensitivity to ivermectin and pyrantel of Ancylostoma ceylanicum and Necator americanus. Int J Parasitol 23 : 945–952.
  26. Richards JC, Behnke JM, Duce IR, 1995. In vitro studies on the relative sensitivity to ivermectin of Necator americanus and Ancylostoma ceylanicum. Int J Parasitol 25 : 1185–1191.
  27. Kopp SR, Coleman GT, McCarthy JS, Kotze AC, 2008. Phenotypic characterization of two Ancylostoma caninum isolates of different susceptibility to the anthelmintic pyrantel. Antimicrob Agents Chemother 52 : 3980–3986.
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  • Received : 30 Jan 2009
  • Accepted : 24 Mar 2009

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