1921
Volume 81, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

In this study, we evaluate the MM3-COPRO method for detection of coproantigens in human fecal samples, and the usefulness of a new preservative/diluent, CoproGuard, developed for preservation of coproantigens. The MM3-COPRO assay was evaluated with 213 samples from healthy patients, 30 positive fecal samples (according to the Kato-Katz method), and 83 samples from patients with other parasitic infections. All positive specimens were detected with the MM3-COPRO assay (100% sensitivity) and there was no cross-reactivity with other common parasites present in the clinical specimens analyzed (100% specificity). The use of CoproGuard enhanced coproantigen extraction without affecting the detection limit of the assay, and the antigenicity of coproantigens in fecal samples stored at 37°C was retained throughout the entire observation period (120 days). We concluded that the MM3-COPRO ELISA combined with the use of CoproGuard may be a very useful tool for the diagnosis of human fascioliasis.

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2009-07-01
2017-11-22
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  • Received : 19 Jan 2009
  • Accepted : 16 Mar 2009

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