1921
Volume 80, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

During the monitoring of arbovirus seroprevalence in wild birds collected in California, we inadvertently made two isolates of western equine encephalomyelitis virus (WEEV) from California quail sera being tested by plaque reduction neutralization assay for antibodies against St Louis encephalitis (SLEV) and West Nile (WNV) viruses despite heating the sera at 56°C for 30 minutes. These data prompted us to examine the thermostability of these viruses during heat treatment. The flaviviruses, SLEV and WNV, at titers up to 10 plaque-forming units (PFU), were readily inactivated by the standard protocol of heating at 56°C for 30 minutes. In contrast, solutions containing 10 and 10 PFU of WEEV required 2 hours for complete inactivation. Occasional presence of live virus within sera could lead to false negatives using standard plaque reduction neutralization test protocols.

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/content/journals/10.4269/ajtmh.2009.80.862
2009-05-01
2017-11-21
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References

  1. World Health Organization, 2007. Guidelines for Plaque-Reduction Neutralization Testing of Human Antibodies to Dengue Viruses. Geneva: World Health Organization.
  2. Chiles RE, Reisen WK, 1998. A new enzyme immunoassay to detect antibodies to arboviruses in the blood of wild birds. J Vector Ecol 23 : 123–135.
  3. Reisen WK, Chiles RE, Martinez VM, Fang Y, Green EN, 2003. Experimental infection of California birds with western equine encephalomyelitis and St. Louis encephalitis viruses. J Med Entomol 40 : 968–982.
  4. Reisen WK, Fang Y, Brault AC. 2008. Limited interdecadal variation in mosquito (Diptera: Culicidae) and avian host competence for Western equine encephalomyelitis virus (Togaviridae: Alphavirus). Am J Trop Med Hyg 78 : 681–686.
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  • Received : 18 Nov 2008
  • Accepted : 16 Jan 2009

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