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Volume 80, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

A morphologic study of the lungs was carried out in Swiss mice infected with yeast isolated from (Pb18). The lung was processed 1, 2, 4, and 8 weeks after inoculation for histologic staining with hematoxylin and eosin (H&E), methenamine silver nitrate (Gomori–Grocott), and picrosirius to qualitative and quantitative analyses of the granulomas and the presence of fungal lesions. The numbers of CFUs/g counted in the lungs were 189.8 ± 20.64, 353.6 ± 46.21, 547.2 ± 108.1, and 295.2 ± 89.17 in the first, second, fourth, and eighth weeks, respectively. One week after infection, inflammatory cells and reticular and collagens fibers, the latest typical of fibrosis, were detected. After 2 and 4 weeks, a progressive intensification of the infection and fibrosis was observed, but in week 8 a more organized granuloma was evident, with macrophages, epithelioid cells, and yeasts in the central portion, and intense peripheral basophilia. Pycnotic structures typical of apoptotic bodies were observed in weeks 1 and 8. The different histologic staining used acted as a fundamental tool for the study of the morphologic organization of granuloma formation.

Copyright 2009 The American Society of Tropical Medicine
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2009-05-01
2018-06-20

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References

  1. Calich VL, Vaz CA, Burger E, 1998. Immunity to Paracoccidioides brasiliensis infection. Res Immunol 149 : 407–417.
  2. Lutz A, 1908. Uma micose pseudococcidica localizada na boca e observada no Brasil. Contribuição ao conhecimento das hyphoblastomycoses americanas. Bras Med 22 : 121–124.
  3. McEwen JG, Bedoya V, Patiño MM, Salazar ME, Restrepo A, 1987. Experimental murine paracoccidioidomycosis induced by the inhalation of conidia. J Med Vet Mycol 25 : 165–175.
  4. Del Negro G, Lacaz CS, Zamith VA, Siqueira AM, 1994. General clinical aspects: polar forms of paracoccidioidomycosis, the disease in childhood. Franco M, Lacaz CS, Restrepo-Moreno A, Del Negro G, eds. Paracoccidioidomycosis. Boca Raton, FL: CRC Press, 225–232.
  5. Franco M, 1987. Host-parasite relationships in paracoccidioidomycosis. J Med Vet Mycol 25 : 5–18.
  6. Shikanai-Yasuda MA, Telles F, de Queiroz F, Mendes RP, Colombo AL, Moretti ML, 2006. Consenso em paracoccidioidomicose. Rev Soc Bras Med Trop 39 : 297–310.
  7. Franco L, Najvar L, Gomez BL, Restrepo S, Graybill JR, Restrepo A, 1998. Experimental pulmonary fibrosis induced by Paracoccidioides brasiliensis conidia: measurement of local host responses. Am J Trop Med Hyg 58 : 424–430.
  8. Cock AM, Cano LE, Vélez D, Aristizábal BH, Trujillo J, Restrepo A, 2000. Fibrotic sequelae in pulmonary paracoccidioidomycosis: histopathological aspects in BALB/c mice infected with viable and non-viable Paracoccidioides brasiliensis propagules. Rev Inst Med Trop Sao Paulo 42 : 59–66.
  9. Vilani-Moreno F, Fecchio D, de Mattos MC, Moscardi-Bacchi M, Defaveri J, Franco M, 1998. Study of pulmonary experimental paracoccidioidomycosis by analysis of bronchoalveolar lavage cells: resistant vs. susceptible mice. Mycopathologia 141 : 79–91.
  10. Nishikaku AS, Burger E, 2003. Immunohistochemical demonstration of TGF-β and decorin in paracoccidioidal granulomas. Braz J Med Biol Res 36 : 1073–1078.
  11. Franco M, Peracoli MT, Soares A, Montenegro R, Mendes RP, Meira DA, 1993. Host-parasite relationship in paracoccidioidomycosis. Curr Top Med Mycol 5 : 115–149.
  12. Bitterman PB, Adelberg S, Crystal RG, 1983. Mechanisms of pulmonary fibrosis. Spontaneous release of the alveolar macrophage-derived growth factor in the interstitial lung disorders. J Clin Invest 72 : 1801–1813.
  13. Campos EP, Torchio LN, Cataneo AJM, Perotti EA, 1986. Paracoccidioidomicose pulmonar agressiva, endotraqueíte estenosante e Corpulmonale subagudo. Descrição de um caso. Rev Inst Med Trop Sao Paulo 28 : 185–189.
  14. Friedman SL, 1993. Seminars in medicine of the Beth Israel Hospital, Boston. The cellular basis of hepatic fibrosis. Mechanisms and treatment strategies. N Engl J Med 328 : 1828–1835.
  15. Kerr IB, Araripe JR, Oliveira PC, Lenzi HL, 1988. Paracoccidioidomycosis: a sequential histopathologic study of lesions in experimentally-infected rats. Rev Inst Med Trop Sao Paulo 30 : 336–350.
  16. Figueiredo F, Silva CLM, Rossi MA, 1986. Participation of Paracoccidioides brasiliensis lipids and polysaccharides in the evolution of granulomas. Braz J Med Biol Res 19 : 651–653.
  17. Kerr IB, de Oliveira PC, Lenzi HL, 1988. Connective matrix organization in chronic granulomas of experimental paracoccidioidomycosis. Mycopathologia 103 : 11–20.
  18. Moscardi-Bacchi M, Franco M, 1985. Experimental paracoccidioidomycosis in the mouse. III. Histopathological and immunological findings after intravenous infection in the presence or absence of previous immunization. Rev Soc Bras Med Trop 18 : 101–108.
  19. Franco M, Montenegro MR, Mendes RP, Marques SA, Dillon NL, Mota NGS, 1987. Paracoccidioidomycosis: a recently proposed classification of its clinical forms. Rev Soc Bras Med Trop 20 : 129–132.
  20. Moscardi-Bacchi M, Franco MF, 1981. Experimental paracoccidioidomycosis in mice. II. Intraperitoneal infection after previous sensitization. Rev Inst Med Trop Sao Paulo 23 : 204–211.
  21. Singer-Vermes LM, Caldeira CB, Burger E, Calich VL, 1993. Experimental murine paracoccidioidomycosis: relationship among the dissemination of the infection, humoral and cellular immune responses. Clin Exp Immunol 94 : 75–79.
  22. Grocott RG, 1955. A stain for fungi in tissue sections and smears using Gomori’s methenamine–silver nitrate technic. Am J Clin Pathol 25 : 975–979.
  23. Junqueira LC, Bignolas G, Brentani RR, 1979. Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections. Histochem J 11 : 447–455.
  24. Dolber PC, Spach MS, 1993. Conventional and confocal fluorescence microscopy of collagen fibers in the heart. J Histochem Cytochem 41 : 465–469.
  25. André DC, Lopes JD, Franco MF, Vaz CA, Calich VL, 2004. Binding of laminin to Paracoccidioides brasiliensis induces a less severe pulmonary paracoccidioidomycosis caused by virulent and low-virulence isolates. Microbes Infect 6 : 549–558.
  26. Moscardi-Bacchi M, Soares A, Mendes R, Marques S, Franco M, 1989. In situ localization of T lymphocyte subsets in human paracoccidioidomycosis. J Med Vet Mycol 27 : 149–158.
  27. Roitt I, Brostoff J, Male D, 1998. Hypersensitivity – Type IV, in Immunology, (Fifth edition). London: Mosby International Ltd., 341–348.
  28. Bagagli E, Sano A, Coelho KI, Alquati S, Miyaji M, Camargo ZP, Gomes GM, Franco M, Montenegro MR, 1998. Isolation of Paracoccidioides brasiliensis from armadillos (Dasypus noveminctus) captured in an endemic area of paracoccidioidomycosis. Am J Trop Med Hyg 58 : 505–512.
  29. Essayag SM, Landaeta ME, Hartung C, Magaldi S, Spencer L, Suárez R, García F, Pérez E, 2002. Histopathologic and histochemical characterization of calcified structures in hamsters inoculated with Paracoccidioides brasiliensis. Mycoses 45 : 351–357.
  30. Mendes-Giannini MJ, Hanna SA, da Silva JL, Andreotti PF, Vincenzi LR, Benard G, Lenzi HL, Soares CP, 2004. Invasion of epithelial mammalian cells by Paracoccidioides brasiliensis leads to cytoskeletal rearrangement and apoptosis of the host cell. Microbes Infect 6 : 882–891.
  31. Dias MF, Filgueira AL, de Souza W, 2004. A morphological and cytochemical study of the interaction between Paracoccidioides brasiliensis and neutrophils. Microsc Microanal 10 : 215–223.
  32. Tuder RM, el Ibrahim R, Godoy CE, De Brito T, 1985. Pathology of the human pulmonary paracoccidioidomycosis. Mycopathologia 92 : 179–188.
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Morphologic Organization of Pulmonary Granulomas in Mice Infected with Paracoccidioides brasiliensis
The American Journal of Tropical Medicine and Hygiene 80, 798 (2009); https://doi.org/10.4269/ajtmh.2009.80.798
/content/journals/10.4269/ajtmh.2009.80.798
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  • Received : 19 Mar 2008
  • Accepted : 26 Nov 2008

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