1921
Volume 80, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Few genetic markers have been described to analyze populations of . The genetic variability of has been analyzed mainly among isolates taken from areas ranging from hyper- to holoendemic areas. These studies of genetic variability have neglected many areas with different epidemiologic profiles. The purpose of this study was to analyze the genetic variability of isolates from four different Brazilian Amazon areas. We chose to study the five most polymorphic tandem repeats (TRs) identified so far. All TRs studied were polymorphic in at least one studied population, with a modal allele at nearly all loci. Expected heterozygosity ranged from 0.462 to 0.666 and did not correlate with the repeat array length. The genetic distances among the populations varied from 0.027 to 0.241, and did not correlate with their geographic separation. Tandem repeats identified in isolates failed to allow geographic clustering.

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2009-05-01
2017-09-25
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References

  1. Price R, Tjitra E, Guerra C, Yeung S, White N, Anstey N, 2007. Vivax malaria: neglected and not benign. Am J Trop Med Hyg 77 : 79–87.
  2. Health HMoBSoVi, 2007. Malaria epidemiologic situation in Brazil.
  3. Mendis K, Sina B, Marchesini P, Carter R, 2001. The neglected burden of Plasmodium vivax malaria. Am J Trop Med Hyg 64 : 97–106.
  4. Carlton J, 2003. The Plasmodium vivax genome sequencing project. Trends Parasitol 19 : 227–231.
  5. Russell B, Suwanarusk R, Lek-Uthai U, 2006. Plasmodium vivax genetic diversity: microsatellite length matters. Trends Parasitol 22 : 399–401.
  6. Golenda C, Li J, Rosenberg R, 1997. Continuous in vitro propagation of the malaria parasite Plasmodium vivax. Proc Natl Acad Sci USA 94 : 6786–6791.
  7. del Portillo H, Longacre S, Khouri E, David P, 1991. Primary structure of the merozoite surface antigen 1 of Plasmodium vivax reveals sequences conserved between different Plasmodium species. Proc Natl Acad Sci USA 88 : 4030–4034.
  8. Bruce M, Galinski M, Barnwell J, Snounou G, Day K, 1999. Polymorphism at the merozoite surface protein-3alpha locus of Plasmodium vivax: global and local diversity. Am J Trop Med Hyg 61 : 518–525.
  9. Jarne P, David P, Viard F, 1998. Microsatellites, transposable elements and the X chromosome. Mol Biol Evol 15 : 28–34.
  10. Gomez J, McNamara D, Bockarie M, Baird J, Carlton J, Zimmerman P, 2003. Identification of a polymorphic Plasmodium vivax microsatellite marker. Am J Trop Med Hyg 69 : 377–379.
  11. Leclerc M, Durand P, Gauthier C, Patot S, Billotte N, Menegon M, Severini C, Ayala F, Renaud F, 2004. Meager genetic variability of the human malaria agent Plasmodium vivax. Proc Natl Acad Sci USA 101 : 14455–14460.
  12. Imwong M, Sudimack D, Pukrittayakamee S, Osorio L, Carlton J, Day N, White N, Anderson T, 2006. Microsatellite variation, repeat array length, and population history of Plasmodium vivax. Mol Biol Evol 23 : 1016–1018.
  13. Imwong M, Nair S, Pukrittayakamee S, Sudimack D, Williams J, Mayxay M, Newton P, Kim J, Nandy A, Osorio L, Carlton J, White N, Day N, Anderson T, 2007. Contrasting genetic structure in Plasmodium vivax populations from Asia and South America. Int J Parasitol 37 : 1013–1022.
  14. Karunaweera ND, Ferreira MU, Hartl DL, Wirth DF, 2007. Fourteen polymorphic microsatellite DNA markers for the human malaria parasite Plasmodium vivax. Mol Ecol Notes 7 : 172–175.
  15. Feng X, Carlton J, Joy D, Mu J, Furuya T, Suh B, Wang Y, Barnwell J, Su X, 2003. Single-nucleotide polymorphisms and genome diversity in Plasmodium vivax. Proc Natl Acad Sci USA 100 : 8502–8507.
  16. Karunaweera N, Ferreira M, Munasinghe A, Barnwell J, Collins W, King C, Kawamoto F, Hartl D, Wirth D, 2008. Extensive micro-satellite diversity in the human malaria parasite Plasmodium vivax. Gene 410 : 105–112.
  17. Camargo L, Ferreira M, Krieger H, De Camargo E, Da Silva L, 1994. Unstable hypoendemic malaria in Rondonia (western Amazon region, Brazil): epidemic outbreaks and work-associated incidence in an agro-industrial rural settlement. Am J Trop Med Hyg 51 : 16–25.
  18. Health HMoBSoVi, 2005. Malaria epidemiologic situation in Brazil.
  19. Excoffier L, Laval G, Schneider S, 2005. Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online, 47–50.
  20. Haubold B, Hudson R, 2000. LIAN 3.0: detecting linkage disequilibrium in multilocus data. Linkage analysis. Bioinformatics 16 : 847–848.
  21. Ferreira M, Karunaweera N, da Silva-Nunes M, da Silva N, Wirth D, Hartl D, 2007. Population structure and transmission dynamics of Plasmodium vivax in rural Amazonia. J Infect Dis 195 : 1218–1226.
  22. Mantel N, 1967. Detection of disease clustering and a generalized regression approach. Cancer Res 27 : 209.
  23. Pritchard J, Stephens M, Donnelly P, 2000. Inference of population structure using multilocus genotype data. Genetics 155 : 945–959.
  24. Machado R, Povoa M, Calvosa V, Ferreira M, Rossit A, dos Santos E, Conway D, 2004. Genetic structure of Plasmodium falciparum populations in the Brazilian Amazon region. J Infect Dis 190 : 1547–1555.
  25. Anderson T, Haubold B, Williams J, Estrada-Franco J, Richardson L, Mollinedo R, Bockarie M, Mokili J, Mharakurwa S, French N, Whitworth J, Velez I, Brockman A, Nosten F, Ferreira M, Day K, 2000. Microsatellite markers reveal a spectrum of population structures in the malaria parasite Plasmodium falciparum. Mol Biol Evol 17 : 1467–1482.
  26. Leclerc M, Gauthier C, Villegas L, Urdaneta L, 2005. Genetic diversity of merozoite surface protein-1 gene of Plasmodium vivax isolates in mining villages of Venezuela (Bolivar State). Acta Trop 95 : 26–32.
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  • Received : 22 Jul 2008
  • Accepted : 02 Dec 2008

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