- Authors: Scott J. Balsitis1, Josefina Coloma1, Glenda Castro1, Aracely Alava1, Diana Flores1, James H. McKerrow1, P. Robert Beatty1, Eva Harris1,*
View Affiliations Hide AffiliationsAffiliations: 1 Division of Infectious Diseases, School of Public Health, and Department of Molecular and Cell Biology, University of California, Berkeley, California; Hospital de Infectología, Ministerio de Salud, Guayaquil, Ecuador; Instituto Nacional de Higiene, Ministerio de Salud, Guayaquil, Ecuador; Sandler Center for Basic Research in Parasitic Diseases, University of California, San Francisco, California
- Publisher: The American Society of Tropical Medicine and Hygiene,
- Source: The American Journal of Tropical Medicine and Hygiene, Volume 80, Issue 3, Mar 2009, p. 416 - 424
- DOI: https://doi.org/10.4269/ajtmh.2009.80.416
f Tropism of Dengue Virus in Mice and Humans Defined by Viral Nonstructural Protein 3-Specific Immunostaining
Previous attempts to define dengue virus (DENV) tropism in human autopsy tissues have detected DENV antigens that are abundant in circulation during severe dengue, and thus may be present in uninfected cells. To better define DENV tropism, we performed immunostaining for the DENV2 nonstructural protein 3 (NS3) in humans and in a mouse model of DENV infection. In mice, NS3 was detected in phagocytes of the spleen and lymph node, hepatocytes in liver, and myeloid cells in bone marrow. In human autopsy tissues, NS3 was present in phagocytes in lymph node and spleen, alveolar macrophages in lung, and perivascular cells in brain. This protein was also found in hepatocytes in liver and endothelial cells in spleen, although NS3 was not present in endothelium in any other tissue. Thus, NS3-specific immunostaining supports roles for infected phagocytes, hepatocytes, and, to a limited degree, endothelial cells in the pathogenesis of severe dengue.
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