Volume 80, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


We studied the effects of male age, body size, and density on mating success under laboratory and field conditions. Older males under field conditions transferred the greatest number of sperm to females (1,152 by 1-day-old males to 1,892 sperm by 10-day-old males). Larger males inseminated females with more sperm than smaller ones. Male age, female body size, and density also influenced male mating success. Larger females successfully mated with males more often than smaller females, especially with older males (> 25 days old). Female insemination rates in small high-density laboratory cages (0.009 m) were artificially high (81.6–98.7%) compared with rates (65.4–84.6%) in large low-density field cages (9 m). This is the first study to systematically evaluate the effect of male body size and age on sperm transfer to females and the first one to evaluate the mating performance of males in a field setting.


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  1. Gubler DJ, 2002. Epidemic dengue/dengue hemorrhagic fever as a public health, social and economic problem in the 21st century. Trends Microbiol 10 : 100–103.
  2. Clements AN, 1999. The Biology of Mosquitoes: Sensory Reception and Behaviour. Wallingford: CABI Publishing.
  3. Hartberg W, 1971. Observations on the mating behaviour of Aedes aegypti in nature. Bull World Health Organ 45 : 847–850.
  4. Jones JC, Wheeler RE, 1965. An analytical study of coitus in Aedes aegypti (Linnaeus). J Morphol 117 : 401–423.
  5. Roth LM, 1948. A study of mosqutio behavior. An experimental laboratory study of the sexual behaviour of Aedes aegypti Linnaeus. Am Midl Nat 40 : 265–352.
  6. Spielman A, 1964. The mechanics of copulation in Aedes aegypti. Biol Bull 127 : 324–344.
  7. Sirot LK, Poulson RL, McKenna MC, Girnary H, Wolfner MF, Harrington LC, 2008. Identity and transfer of male reproductive gland proteins of the dengue vector mosquito, Aedes aegypti: potential tools for control of female feeding and reproduction. Insect Biochem Mol Biol 38 : 176–189.
  8. Partridge L, Hoffmann A, Jones J, 1987. Male size and mating success in Drosophila melanogaster and D. pseudoobscura under field conditions. Anim Behav 35 : 468–476.
  9. Pitnick S, Garcia-Gonzalez F, 2002. Harm to females increases with male body size in Drosophila melanogaster. Proc Biol Sci 269 : 1821–1828.
  10. Renshaw M, Service MW, Birley MH, 1994. Size variation and reproductive success in the mosquito Aedes cantans. Med Vet Entomol 8 : 179–186.
  11. Ponlawat A, Harrington LC, 2007. Age and body size influence male sperm capacity of the dengue vector Aedes aegypti (Diptera: Culicidae). J Med Entomol 44 : 422–426.
  12. Yuval B, Wekesa J, Washino R, 1993. Effects of body size on swarming behaviour and mating success of male Anopheles freeborni (Diptera: Culicidae). J Insect Behav 6 : 332–342.
  13. Okanda FM, Dao A, Njiru BN, Arija J, Akelo HA, Toure Y, Odulaja A, Beier JC, Githure JI, Yan G, Gouagna LC, Knols BG, Killeen GF, 2002. Behavioural determinants of gene flow in malaria vector populations: Anopheles gambiae males select large females as mates. Malar J 1 : 10.
  14. Charlwood JD, Pinto J, Sousa CA, Madsen H, Ferreira C, do Rosario VE, 2002. The swarming and mating behaviour of Anopheles gambiae s.s. (Diptera: Culicidae) from Sao Tome Island. J Vector Ecol 27 : 178–183.
  15. Charlwood JD, Thompson R, Madsen H, 2003. Observations on the swarming and mating behaviour of Anopheles funestus from southern Mozambique. Malar J 2 : 2.
  16. Delisle J, 1995. Effect of male and female age on the mating success of the obliquebanded leafroller Choristoneura rosaceana (Lepidoptera: Tortricidae) under different ecological conditions. Insect Behavior 8 : 781–799.
  17. Zuk M, 1988. Parasite load, body size, and age of wild-caught male field crickets (Orthoptera: Gryllidae): effects on sexual selection. Evol Int J Org Evol 42 : 969–976.
  18. Olet P, Opiyo E, Robinson A, 2002. Sexual receptivity and age in Glossina pallidipes Austen (Dipt., Glossinidae). J Appl Entomol 126 : 86–91.
  19. Taylor P, Kaspi R, Mossinson S, Yuval B, 2001. Age-dependent insemination success of sterile Mediterranean fruit flies. Entomol Exp Appl 98 : 27–33.
  20. Rattanarithikul R, Panthusiri P, 1994. Illustrated keys to the medically important mosquitoes of Thailand. Southeast Asian J Trop Med Pub Health 1–25.
  21. Chevone B, Richards A, 1976. Ultrastructure of the atypic muscles associated with terminalial inversion in male Aedes aegypti (L). Biol Bull 151 : 283–296.
  22. Scott TW, Amerasinghe PH, Morrison AC, Lorenz LH, Clark GG, Strickman D, Kittayapong P, Edman JD, 2000. Longitudinal studies of Aedes aegypti (Diptera: Culicidae) in Thailand and Puerto Rico: blood feeding frequency. J Med Entomol 37 : 89–101.
  23. Nasci RS, 1990. Relationship of wing length to adult dry weight in several mosquito species (Diptera: Culicidae). J Med Entomol 27 : 716–719.
  24. Ziegler A, Arminger G, 1996. Parameter estimation and regression diagnostics using generalized estimating equations. Faulbaum Faulbeam, F., ed. SoftStat ‘95 Advances in Statistical Software 5. Stuttgart: Lucius & Lucius, 229–237.
  25. Ponlawat A, Harrington L, 2005. Blood feeding patterns of Aedes aegypti and Aedes albopictus in Thailand. J Med Entomol 42 : 844–849.
  26. Curtis C, 1985. Genetic control of insects: growth industry or lead balloon? Biol J Linn Soc 26 : 359–374.
  27. Cabrera M, Jaffe K, 2007. An aggregation pheromone modulates lekking behavior in the vector mosquito Aedes aegypti (Diptera: Culicidae). J Am Mosq Control Assoc 23 : 1–10.

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  • Received : 19 Mar 2008
  • Accepted : 17 Oct 2008

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