Volume 80, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Restriction fragment length polymorphism (RFLP) analysis using a multilocus heat-inducible cytoplasmic heat-shock protein 70 (Hsp70) hybridization probe with RI-digested genomic DNA was used in molecular typing of 129 isolates. Results indicate that organisms exhibit considerable polymorphism in their Hsp70 RFLP patterns. Analysis of seven American Type Culture Collection reference strains and 122 clinical isolates, including 84 isolates from Jackson, Mississippi, 18 isolates from Atlanta, Georgia, and 20 isolates from throughout the United States, showed 105 distinct Hsp70 RFLP pattern subtypes for . Phylogenetic analysis of the Hsp70 RFLP data showed that the isolates were organized into two clonal lineages. These results illustrate the substantial genomic diversity present in and indicate that a large number of genetically distinct isolates may be responsible for human trichomoniasis in the United States.


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  • Received : 01 May 2008
  • Accepted : 11 Oct 2008

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