1921
Volume 80, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Stool samples from 86 immunocompromised patients (51 human immunodeficiency virus (HIV)–infected patients and 35 patients with haematologic malignancies) were systematically screened for intestinal microspordiosis by microscopic examination and polymerase chain reaction (PCR) using universal primer V1/PMP2. Nine samples (10.5%) showed amplification with the predictive size of fragment (6 from HIV–infected patients and 3 from patients with myeloma). Only 5 out of them (all HIV–infected patients) were revealed positive by microscopy. By means of amplicons fragment size, species–specific primers (V1/EB450, V1/IS500) and sequencing, 3 microsporidia species were for the first time identified in Tunisia: (3 isolates), (2 isolates), and (1 isolate). Systematic use of such sensitive and discriminative molecular tools will contribute to determining the true prevalence of microsporidiosis in Tunisia and to better management of infected immunocompromised subjects.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.2009.80.24
2009-01-01
2017-09-20
Loading full text...

Full text loading...

/deliver/fulltext/14761645/80/1/0800024.html?itemId=/content/journals/10.4269/ajtmh.2009.80.24&mimeType=html&fmt=ahah

References

  1. Molina JM, Sarfati C, Beauvais B, Lemann M, Lesourd F, Ferchal F, Casin I, Lagrange P, Modigliani R, Derouin F, Modai J, 1993. Intestinal microsporidiosis in human immunodeficiency virus-infected patients with chronic unexplained diarrhea: prevalence and clinical and biologic features. J Infect Dis 167 : 217–221.
  2. Guerard A, Rabodonirina M, Cotte L, Liguory O, Piens MA, Daoud S, Picot S, Touraine JL, 1999. Intestinal microsporidiosis occurring in two renal transplant recipients treated with mycophenolate mofetil. Transplantation 5 : 699–707.
  3. Weber R, Bryan RT, 1992. Improved light-microspridial detection of microsporidia spores in stool and duodenal aspirates. N Engl J Med 326 : 161–166.
  4. Katzwinkel-Wladarsch S, Lieb M, Helse W, Loscher T, Rindier H, 1996. Direct amplification and species determination of microsporidian DNA from stool specimens. Top Med Int Health 1 : 373–378.
  5. Didier ES, 1997. Effects of Albendazole, Fumagillin, and TMP-470 on microsporidial replication in vitro. Antimicrob Agents Chemother 41 : 1541–1546.
  6. Weber R, Bryan RT, Schwartz DA, Owen RL, 1994. Human microsporidial infections. Clin Microbiol Rev 7 : 426–461.
  7. Franzen C, Schwartz DA, Visvesvara GS, Muller A, Schwenk A, Salzberger B, Fatkenheur G, Hartmann P, Diehl V, Schrappe M, 1995. Immunologically confirmed disseminated asymptomatic Encephalitozoon cuniculi infection of gastrointestinal tract in patient with AIDS. Clin Infect Dis 21 : 1480–1484.
  8. Belhadj S, Kallel K, Boussen N, Ghobatini A, Bejoui M, Ben salem N, Ziribi A, BenChaabane T, Chaker E, 1999. Places des cryptosporidies et des microsporidies dans les diarrhées des immunodéprimés. Tunis Med 77 : 638–643.
  9. Aoun K, Bouratbine A, Darty A, Biligui S, Ben Ismail R, 1997. Présence de microsporidies intestinales en Tunisie: à propos d’un cas. Bull Soc Path Ex 90 : 176.
  10. Kokoskin E, Gyorkos TW, Camus A, Cedilotte L, Purtill T, Ward B, 1994. Modified technique for efficient detection of microsporidia. J Clin Microbiol 36 : 1974–1975.
  11. Fedorko DP, Nelson NA, Cartwright CP, 1995. Identification of microsporidiabin stool specimens by using PCR and restriction endonucleases. J Clin Microbiol 33 : 1739–1741.
  12. Coyle CM, Wittner M, Kotler DP, Noyer C, Orenstein JM, Tanowitz HB, Weiss LM, 1996. Prevalence of microsporidiosis due to Enterocytozoon bieneusi and Encephalitozoon (Septata) intestinalis among patients with AIDS-related diarrhea: determination by chain polymerase reaction to the microsporidian small-subunit rRNA gene. Clin Infect Dis 23 : 1002–1006.
  13. Müller A, Stellerman K, Hartmann P, Scharppa M, Fathenheuer G, Salzberger B, Diehl V, Franzen C, 1999. A powerful DNA extraction method and PCR for detection of mocrosporidia in clinical stool specimens. Clin Diagn Lab Immunol 2 : 243–246.
  14. Didier ES, Orenstein JM, Aldras A, Bertucci D, Rogers LB, Janney FA, 1995. Comparaison of three methods for detecting microsporidia in fluids. J Clin Microbiol 33 : 3138–3145.
  15. Dowd SE, Gerba CP, Pepper IL, 1998. Confirmation of human-pathogenic microsporidia Enterocytozoon bieneusi, Encephalitozoon intestinalis and Vittaforma corneae in water. Appl Environ Microbiol 64 : 3332–3335.
  16. Cali A, Kotler P, Orenstein JM, 1993. Septata intestinalis N. G., N. sp., an intestinal microsporidian associated with chronic diarrhea and dissemination in AIDS patients. J Eukaryot Microbiol 40 : 101–112.
  17. Didier ES, Didier PJ, Friedberg DN, Stenson SM, Orenstein JM, Yee RW, Tio FO, Davis MR, Vossbrinck C, Millichamp N, Shadduck JA, 1991. Isolation and characterization of a new human microsporidia, Encephalitozoon hellem (n. sp), from three AIDS patients with keratoconjunctivitis. J Infect Dis 163 : 617–621.
  18. Schwartz DA, Visvesvara GS, Leitch GJ, Tashjian L, Pollack M, Holden J, Bryan RT, 1993. Pathology of symptomatic microsporidial (Encephalitozoon hellem) bronchiolitis in AIDS: a new respiratory pathogen diagnosed from lung biopsy, bronchoalveolar lavage, sputum, and tissue culture. Hum Pathol 24 : 937–943.
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.2009.80.24
Loading
/content/journals/10.4269/ajtmh.2009.80.24
Loading

Data & Media loading...

  • Received : 04 Jul 2008
  • Accepted : 20 Aug 2008

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error