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Volume 80, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

No cause of one-third of travelers’ diarrhea (TD) cases can be detected despite microbiologic assessment. We propose that these pathogen-negative TD cases include undetected enterotoxigenic (ETEC). As a standard in diagnostic microbiology, samples of five colonies were tested to detect ETEC from stool cultures. We compared the sensitivities and the number of ETEC detected by 20 colonies with those of 5, 10, and 15 colonies. The percent detection of ETEC with five colonies was significantly different from that using 20 colonies. Of the 116 subjects studied, the number positive for ETEC for 5, 10, 15 and 20 colonies tested were 22 (19.0%), 37 (31.9%), 45 (38.8%) and 46 (39.7%), respectively. Based on our results, we recommend the testing of at least 10 colonies for optimum detection of ETEC in patients with TD.

Copyright 2009 The American Society of Tropical Medicine
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2009-01-01
2018-11-21

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References

  1. Jiang ZD, Lowe B, Verenkar MP, Ashley D, Steffen R, Tornieporth N, von Sonnenburg F, Waiyaki P, DuPont HL, 2002. Prevalence of enteric pathogens among international travelers with diarrhea acquired in Kenya (Mombasa), India (Goa), or Jamaica (Montego Bay). J Infect Dis 185 : 497–502.
  2. Ko G, Garcia C, Jiang ZD, Okhuysen PC, Belkind-Gerson J, Glass RI, DuPont HL, 2005. Noroviruses as a cause of traveler’s diarrhea among students from the United States visiting Mexico. J Clin Microbiol 43 : 6126–6129.
  3. Paredes P, Campbell-Forrester S, Mathewson JJ, Ashley D, Thompson S, Steffen R, Jiang ZD, Svennerholm AM, DuPont HL, 2000. Etiology of travelers’ diarrhea on a Caribbean island. J Travel Med 7 : 15–18.
  4. Sanchez J, Holmgren J, 2005. Virulence factors, pathogenesis and vaccine protection in cholera and ETEC diarrhea. Curr Opin Immunol 17 : 388–398.
  5. Begaud E, Germani Y, 1992. Detection of enterotoxigenic Escherichia coli in faecal specimens by acetylaminofluorene-labelled DNA probes. Res Microbiol 143 : 315–325.
  6. Lund A, Wasteson Y, Olsvik O, 1991. Immunomagnetic separation and DNA hybridization for detection of enterotoxigenic Escherichia coli in a piglet model. J Clin Microbiol 29 : 2259–2262.
  7. Sommerfelt H, Bhan MK, Srivastava R, Bhatnagar S, 1993. Evaluation of DNA-DNA hybridization for the direct detection of enterotoxigenic Escherichia coli in stool blots. Scand J Infect Dis 25 : 457–463.
  8. Caeiro JP, Estrada-Garcia MT, Jiang ZD, Mathewson JJ, Adachi JA, Steffen R, DuPont HL, 1999. Improved detection of enterotoxigenic Escherichia coli among patients with travelers’ diarrhea, by use of the polymerase chain reaction technique. J Infect Dis 180 : 2053–2055.
  9. Ghosal A, Bhowmick R, Nandy RK, Ramamurthy T, Chatterjee NS, 2007. PCR-based identification of common colonization factor antigens of enterotoxigenic Escherichia coli. J Clin Microbiol 45 : 3068–3071.
  10. Stacy-Phipps S, Mecca JJ, Weiss JB, 1995. Multiplex PCR assay and simple preparation method for stool specimens detect enterotoxigenic Escherichia coli DNA during course of infection. J Clin Microbiol 33 : 1054–1059.
  11. Yavzori M, Porath N, Ochana O, Dagan R, Orni-Wasserlauf R, Cohen D, 1998. Detection of enterotoxigenic Escherichia coli in stool specimens by polymerase chain reaction. Diagn Microbiol Infect Dis 31 : 503–509.
  12. Iijima Y, Tanaka S, Miki K, Kanamori S, Toyokawa M, Asari S, 2007. Evaluation of colony-based examinations of diarrheagenic Escherichia coli in stool specimens: low probability of detection because of low concentrations, particularly during the early stage of gastroenteritis. Diagn Microbiol Infect Dis 58 : 303–308.
  13. Black RE, Merson MH, Rowe B, Taylor PR, Abdul Alim AR, Gross RJ, Sack DA, 1981. Enterotoxigenic Escherichia coli diarrhoea: acquired immunity and transmission in an endemic area. Bull World Health Organ 59 : 263–268.
  14. DuPont HL, Olarte J, Evans DG, Pickering LK, Galindo E, Evans DJ, 1976. Comparative susceptibility of Latin American and United States students to enteric pathogens. N Engl J Med 295 : 1520–1521.
  15. DuPont HL, Haake R, Taylor DN, Ericsson CD, Jiang ZD, Okhuysen PC, Steffen R, 2007. Rifaximin treatment of pathogen-negative travelers’ diarrhea. J Travel Med 14 : 16–19.
  16. Huang DB, DuPont HL, 2005. Rifaximin–a novel antimicrobial for enteric infections. J Infect 50 : 97–106.
  17. Jiang ZD, Lowe B, Verenkar MP, Ashley D, Steffen R, Tornieporth N, von Sonnenburg F, Waiyaki P, DuPont HL, 2002. Prevalence of enteric pathogens among international travelers with diarrhea acquired in Kenya (Mombasa), India (Goa), or Jamaica (Montego Bay). J Infect Dis 185 : 497–502.
  18. Persson S, Olsen KE, Scheutz F, Krogfelt KA, Gerner-Smidt P, 2007. A method for fast and simple detection of major diarrhoeagenic Escherichia coli. Clin Microbiol Infect 13 : 516–524.
  19. Grimes KA, Mohamed JA, Dupont HL, Padda RS, Jiang ZD, Flores J, Belkind-Gerson J, Martinez-Sandoval FG, Okhuysen PC, 2008. PCR-based assay using occult blood detection cards for detection of diarrheagenic Escherichia coli in specimens from U.S. travelers to Mexico with acute diarrhea. J Clin Microbiol 46 : 2227–2230.
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Improvement in Detection of Enterotoxigenic Escherichia coli in Patients with Travelers’ Diarrhea by Increasing the Number of E. coli Colonies Tested
The American Journal of Tropical Medicine and Hygiene 80, 20 (2009); https://doi.org/10.4269/ajtmh.2009.80.20
/content/journals/10.4269/ajtmh.2009.80.20
/content/journals/10.4269/ajtmh.2009.80.20
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  • Received : 23 Jul 2008
  • Accepted : 28 Sep 2008

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