1921
Volume 80, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

We examined 364 school children for intestinal parasites in a sub-urban zone of Caracas, Venezuela. was the most prevalent parasite in stool samples from 34 children. Levels of IgA and IgG antibodies to were assessed by enzyme-linked immunosorbent assay and Western blot before and after treatment with secnidazole. All patients were cured with a reduction of IgA antibody levels in 26 of 34 children and a reduction in IgG-specific antibody levels in 18 of 34 children. Serum of infected patients reacted with proteins of 14 kD to 137 kD. Some patients did not show a change in IgA serum reactivity for parasite proteins by Western blot after treatment. Seventeen children showed reduction of the reactivity or disappearance of protein reactivity (mainly the 14-kD, 122-kD, and 137-kD proteins). Antibody response was not related to clinical status, but quantitative and qualitative serum antibody response against infection could be used to assess levels of new protein markers that decrease or disappear with successful chemotherapy.

Loading

Article metrics loading...

The graphs shown below represent data from March 2017
/content/journals/10.4269/ajtmh.2009.80.11
2009-01-01
2019-11-16
Loading full text...

Full text loading...

/deliver/fulltext/14761645/80/1/0800011.html?itemId=/content/journals/10.4269/ajtmh.2009.80.11&mimeType=html&fmt=ahah

References

  1. Adam RD, 1991. The biology of Giardia spp. Microbiol Rev 55 : 706–732. [Google Scholar]
  2. Ahmed MM, El-Hady M, Morsy TA, 1990. Parasitic infections and hemoglobin levels among children of different socioeconomic classes in Abha, Saudi-Arabia. J Egypt Soc Parasitol 20 : 61–67. [Google Scholar]
  3. Di Prisco MC, Jimenez JC, Rodriguez N, Costa V, Villamizar J, Silvera A, Carrillo M, Lira C, Zerpa E, Lopez Y, 2000. Clinical trial with secnidazole in a single dose in Venezuelan children infected by Giardia intestinalis. Invest Clin 41 : 179–188. [Google Scholar]
  4. Miller SA, Rosario CL, Rojas E, Scorza JV, 2003. Intestinal parasitic infection and associated symptoms in children attending day care centers in Trujillo, Venezuela. Trop Med Int Health 8 : 342–347. [Google Scholar]
  5. Heyworth MF, Vergara JA, 1994. Giardia muris trophozoite antigenic targets for mouse intestinal IgA antibody. J Infect Dis 69 : 395–398. [Google Scholar]
  6. Char S, Shetty N, Narasimha M, 1991. Serum antobidy response in children with Giardia lamblia and infection of an immunodominant 57-kilodalton antigen. Parasite Immunol 13 : 329–337. [Google Scholar]
  7. Char S, Cevallo AM, Yamson P, 1993. Impaired IgA response to Giardia heat-shock antigen in children with persistent diarrhoea and giardiasis. Gut 34 : 38–40. [Google Scholar]
  8. Cervetto JL, Ramonet M, Nahmod LH, 1987. Giardiasis. Functional, immunological and histological study of the small bowel. Therapeutic trial with a single dose of tinidazole. Arq Gastroenterol 24 : 102–112. [Google Scholar]
  9. Keister DB, 1983. Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile. Trans R Soc Trop Med Hyg 77 : 487–488. [Google Scholar]
  10. Laemmli UK, 1970. Cleavege of structural proteins during the assembly of the head of bactriophage T4. Nature 227 : 680–685. [Google Scholar]
  11. Towbin H, Staehlin T, Gordon J, 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76 : 4350–4354. [Google Scholar]
  12. Miotti PG, Gilman RH, Pickering LK, 1985. Prevalence of serum and milk antibodies to Giardia lamblia in different populations of lacting women. J Infect Dis 152 : 1025–1031. [Google Scholar]
  13. Rastegar-Lari A, Salek-Moghaddam A, 1996. Single-dose secnidazole versus 10-day metronidazole therapy of giardiasis in Iranian children. J Trop Pediatr 42 : 184–185. [Google Scholar]
  14. Cimerman BL, Camilo Coura C, Salle JM, Gurvitz R, Rocha RS, Bandeira S, Cimerman S, Katz N, 1997. Evaluation of secnidazole gel and tinidazole suspension in the treatment of giardiasis in children. Braz J Infect Dis 1 : 241–247. [Google Scholar]
  15. Escobedo AA, Canete R, Gonzalez ME, Pareja A, Cimerman S, Almirall P, 2003. A randomized trial comparing mebendazole and secnidazole for the treatment of giardiasis. Ann Trop Med Parasitol 97 : 499–504. [Google Scholar]
  16. Jimenez JC, Rodriguez N, Di Prisco MC, Lynch NR, Costa V, 1999. Haemoglobin concentrations and infection by Giardia intestinalis in children: effect of treatment with secnidazole. Ann Trop Med Parasitol 93 : 823–827. [Google Scholar]
  17. Granot E, Spira T, Fraser D, Deckelbaum RJ, 1998. Immunologic response to infection with Giardia lamblia in children: effect of different clinical settings. J Trop Pediatr 44 : 241–246. [Google Scholar]
  18. Hasan SMT, Maachee M, Córdova OM, Diaz de la Guardia R, Martins M, Osuna A, 2002. Human secretory immune response to fatty acid-binding protein fraction from Giardia lamblia. Infect Immun 70 : 2226–2229. [Google Scholar]
  19. Palm JE, Weiland ME, Griffiths WJ, Ljungstrom I, Svard SG, 2003. Identification of immunoreactive proteins during acute human giardiasis. J Infect Dis 187 : 1849–1859. [Google Scholar]
  20. Reiner DS, Gillin F, 1992. Human secretory and serum antibodies recognize environmentally induced antigens of Giardia lamblia. Infect Immun 60 : 637–643. [Google Scholar]
  21. Soliman MM, Ruhi Taghi-Kalani F, Ahmed A, Abou-Shady A, Salama A, El-Mageid A, Hondousa A, Hegazi MM, Belosevic M, 1998. Comparison of serum antibody responses to Giardia lamblia of symptomatic and asymptomatic patients. Am J Trop Med Hyg 58 : 232–239. [Google Scholar]
  22. Craig WR, Walker W, Alexander J, 2001. Sex-associated hormones and immunity to protozoan parasite. Clin Microbiol Rev 14 : 476–488. [Google Scholar]
  23. Bajeva UK, Warhust DC, 1983. Humoral response in giardiasis. J Commun Dis 15 : 256. [Google Scholar]
  24. Anam K, Afrin D, Banerjee N, Pramanik SK, Guha RP, Goswami SK, Saha N, Ali N, 1999. Differential decline in Leishmania membrane antigen-specific immunoglobulin G (IgG), IgM, IgE and IgG subclass antibodies in Indian kalaazar patients after chemotherapy. Infect Immun 67 : 6663–6669. [Google Scholar]
  25. Isaza DM, Restrepo M, Mosca W, 1997. Western blot analysis of Leishmania panamensis antigens in sera of patients with American cutaneous leishmaniasis. J Clin Microbiol 35 : 3043–3047. [Google Scholar]
  26. Kumar Pai PK, Tripathi K, Pandey HP, Sundar S, 2002. Western blot analysis of the humoral immune response to Leishmania donovani polypeptides in cases of human visceral leishmaniasis: its usefulness in prognosis. Clin Diag Lab Immunol 9 : 1119–1123. [Google Scholar]
  27. Mutapi FP, Ndhlovu D, Hagan P, 1998. Chemotherapy accelerates the development of acquired immune response to Schistosoma haematobium infection. J Infect Dis 178 : 289–293. [Google Scholar]
  28. Noya O, Losada S, Alarcón de Noya B, 1995. Effect of chemotherapy on immune response to egg antigens of Schistosoma mansoni in chronically infected children from areas of low transmission. Parasite Immunol 17 : 111–117. [Google Scholar]
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.2009.80.11
Loading
/content/journals/10.4269/ajtmh.2009.80.11
Loading

Data & Media loading...

  • Received : 27 Oct 2007
  • Accepted : 25 Jun 2008

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error