1921
Volume 80, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Currently there are no commercially available selective media indicated for the isolation of and . Ashdown’s agar, a custom selective medium for isolation of is well described in the literature but unavailable commercially. Three commercially available media, selective agar (BCSA), oxidative-fermentative-polymyxin B-bacitracin-lactose (OFPBL) agar, and (PC) agar are recommended for isolation of from respiratory secretions of cystic fibrosis patients. We evaluated the sensitivity and selectivity of these four media using 20 , 20 20 spp., and 15 diagnostically challenging organisms. Ashdown’s agar was the most sensitive medium for the isolation of , but it was unable to support growth of agar was highly sensitive and selective for both organisms. In non-endemic areas, we suggest the use of the commercially available PC agar for the isolation of .

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2009-06-01
2017-09-20
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References

  1. Rotz LD, Khan AS, Lillibridge SR, Ostroff SM, Hughes JM, 2002. Public health assessment of potential bioterrorism agents. Emerg Infect Dis 8 : 225–230.
  2. Ashdown LR, 1979. An improved screening technique for the isolation of Pseudomonas pseudomallei from clinical specimens. Pathology 11 : 293–297.
  3. Wuthiekanun V, Dance DAB, Wattanagoon Y, Supputtamongkol Y, Chaowagul W, White NJ, 1990. The use of selective media for the isolation of Pseudomonas pseudomallei in clinical practice. J Med Microbiol 33 : 121–126.
  4. Peacock SJ, Chieng G, Cheng AC, Dance DAB, Amornchai P, Wongsuvan G, Teerawattanasook N, Chierakul W, Day NPJ, Wuthiekanun V, 2005. Comparison of Ashdown’s medium, Burkholderia cepacia medium, and Burkholderia pseudomallei selective agar for the clinical isolation of Burkholderia pseudomallei. J Clin Microbiol 43 : 5359–5361.
  5. Ashdown LR, Clarke SG, 1992. Evaluation of culture techniques for isolation of Pseudomonas pseudomallei from soil. Appl Environ Microbiol 58 : 4011–4015.
  6. Francis A, Aiyar S, Yean CY, Naing L, Ravichandran M, 2006. An improved selective and differential medium for the isolation of Burkholderia pseudomallei from clinical specimens. Diagn Microbiol Infect Dis 55 : 95–99.
  7. Howard K, Inglis TJJ, 2003. Novel selective medium for isolation of Burkholderia pseudomallei. J Clin Microbiol 41 : 3312–3316.
  8. Henry D, Campbell M, McGimpsey C, Clarke A, Louden L, Burns JL, Roe MH, Vandamme P, Speert D, 1999. Comparison of isolation media for the recovery of Burkholderia cepacia complex from respiratory secretions of patients with cystic fibrosis. J Clin Microbiol 37 : 1004–1007.
  9. Henry DA, Campbell ME, LiPuma JJ, Speert DP, 1997. Identification of Burkholderia cepacia isolates from patients with cystic fibrosis and use of a simple new selective medium. J Clin Microbiol 35 : 614–619.
  10. Welch DF, Muszynski MJ, Pai CH, Marcon MJ, Hribar MM, Gilligan PH, Matsen JM, Ahlin PA, Hilman BC, Chartard SA, 1987. Selective and differential medium for recovery of Pseudomonas cepacia from the respiratory tract of patients with cystic fibrosis. J Clin Microbiol 25 : 1730–1734.
  11. Gilligan PH, Gage PA, Bradshaw LM, Schidlow DV, DeCicco BT, 1985. Isolation medium for the recovery of Pseudomonas cepacia from respiratory secretions of patients with cystic fibrosis. J Clin Microbiol 22 : 5–8.
  12. Weyant RS, Moss CW, Weaver RE, Hollis DG, Jordan JG, Cook EC, Daneshvar MI, 1996. Identification of Unusual Pathogenic Gram-Negative Aerobic and Facultatively Anaerobic Bacteria. Second edition. Baltimore, MD: Williams & Wilkins.
  13. Gee JE, Sacchi CT, Glass MB, De BK, Weyant RS, Levett PN, Whitney AM, Hoffmaster AR, Popovic T, 2003. Use of 16S rRNA gene sequencing for rapid identification and differentiation of Burkholderia pseudomallei and B. mallei. J Clin Microbiol 41 : 4647–4654.
  14. Chantratita N, Wuthiekanun V, Bonbumrung K, Tiyawisutsri R, Vesaratchavest M, Limmathurotsakul D, Chierakul W, White NJ, Day NPJ, Peacock SJ, 2007. Biological relevance of colony morphology and phenotypic switching by Burkholderia pseudomallei. J Bacteriol 189 : 807–817.
  15. Brett PJ, DeShazer D, Woods DE, 1998. Burkholderia thailandensis sp. nov., a Burkholderia pseudomallei-like species. Int J Syst Bacteriol 48 : 317–320.
  16. Glass MB, Steigerwalt AG, Jordon JG, Wilkins PP, Gee JE, 2006. Burkholderia oklahomensis sp. nov., a Burkholderia pseudomallei-like species formerly known as the Oklahoma strain of Pseudomonas pseudomallei. Int J Syst Evol Microbiol 44 : 2171–2176.
  17. Smith MD, Angus BJ, Wuthiekanun V, White NJ, 1997. Arabinose assimilation defines a nonvirulent biotype of Burkholderia pseudomallei. Infect Immun 65 : 4319–4321.
  18. Godoy D, Randle F, Simpson AJ, Aanensen DM, Pitt TL, Kinoshita R, Spratt BG, 2003. Multilocus sequence typing and evolutionary relationships among the causative agents of melioidosis and glanders, Burkholderia pseudomallei and Burkholderia mallei. J Clin Microbiol 41 : 2068–2079.
  19. Payne GW, Vandamme P, Morgan SH, LiPuma JJ, Coenye T, Weightman AJ, Jones TH, Mahenthiralingam E, 2005. Development of a recA gene-based identification approach for the entire Burkholderia genus. Appl Environ Microbiol 71 : 3917–3927.
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  • Received : 25 Aug 2008
  • Accepted : 15 Feb 2009

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