1921
Volume 81, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Clinical trials documented alarming post-treatment recurrence rates caused by recrudescence of surviving asexual blood stages, relapse from hypnozoites, or new infections. Here we describe high rates of recurrence (26–40% 180 days after treatment) in two cohorts of rural Amazonians exposed to low levels of malaria transmission after a vivax malaria episode treated with chloroquine-primaquine. Microsatellite analysis of 28 paired acute infection and recurrence parasites showed only two pairs of identical haplotypes (consistent with recrudescences or reactivation of homologous hypnozoites) and four pairs of related haplotypes (sharing alleles at 11–13 of 14 microsatellites analyzed). Local isolates of were extraordinarily diverse and rarely shared the same haplotype, indicating that frequent recurrences did not favor the persistence or reappearance of clonal lineages of parasites in the population. This fast haplotype replacement rate may represent the typical population dynamics of neutral polymorphisms in parasites from low-endemicity areas.

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Supplementary Table 1

  • Received : 17 Jun 2009
  • Accepted : 31 Jul 2009

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