1921
Volume 81, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

The main malaria vectors of sub-Saharan Africa, sensu stricto and are morphologically indistinguishable, but often occur in sympatry and differ in feeding preference and vector competence. It is important to assess vector species identity for understanding the vectorial system and establishing appropriate vector control measures. The currently available species diagnosis methods for sensu latu require equipment to which public health practitioners in many African countries may not have access. This report describes a loop-mediated isothermal amplification technique (LAMP) for species diagnosis. The LAMP method was tested in single mosquito legs and whole body. The sensitivity and specificity of the LAMP method, in reference to the conventional rDNA-polymerse chain reaction (PCR) method, ranged from 0.93 to 1.00. The LAMP-based species identification method can be performed in a water bath and completed within 65 minutes, representing an alternative method for rapid and field applicable vector species diagnosis.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.2009.09-0333
2009-12-01
2017-09-23
Loading full text...

Full text loading...

/deliver/fulltext/14761645/81/6/0811030.html?itemId=/content/journals/10.4269/ajtmh.2009.09-0333&mimeType=html&fmt=ahah

References

  1. World Health Organization, 2005. Malaria Control Today: Current WHO Recommendations. Geneva: Roll Back Malaria Department.
  2. Coetzee M, Craig M, le Sueur D, 2000. Distribution of African malaria mosquitoes belonging to the Anopheles gambiae complex. Parasitol Today 16: 74–77.
  3. Coluzzi MS, Petrarca V, de Deco MA, 1979. Chromosomal differentiation and adaptation to human environment in the Anopheles gambiae complex. Trans R Soc Trop Med Hyg 73: 479–483.
  4. Coosemans M, Smits A, Roelants P, 1998. Intraspecific isozyme polymorphism of Anopheles gambiae in relation to environment, behavior, and malaria transmission in southwestern Burkina Faso. Am J Trop Med Hyg 58: 70–74.
  5. Carlson DA, Service M, 1979. Differentiation between species of the Anopheles gambiae Giles complex (Diptera: Culicidae) by analysis of cuticular hydrocarbons. Ann Trop Med Parasitol 73: 589–592.
  6. Scott JA, Brogdon W, Collins FH, 1993. Identification of single specimens of the Anopheles gambiae complex by the polymerase chain reaction. Am J Trop Med Hyg 49: 520–529.
  7. Walker ED, Thelen AP, Bullard BA, Huang J, Odiere MR, Bayoh NM, Wilkins EE, Vulule JM, 2007. Identification of field caught Anopheles gambiae s.s. and Anopheles arabiensis by TaqMan single nucleotide polymorphism genotyping. Malar J 6: 23–30.
  8. Bass CW, Field LM, 2008. Development of a multiplex real-time PCR assay for identification of members of the Anopheles gambiae species comples. Acta Trop 107: 50–53.
  9. Parida M, Sannarangaiah S, Dash PK, Rao PV, Morita K, 2008. Loop mediated isothermal amplification (LAMP): a new generation of innovative gene amplification technique; perspectives in clinical diagnosis of infectious diseases. Rev Med Virol 18: 407–421.
  10. Tomita N, Mori YY, Kanda H, Notomi T, 2008. Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products. Nat Protoc 3: 877–882.
  11. Lindblade KA, Gimnig JE, Kamau L, Hawley WA, Odhiambo F, Olang G, Ter Kuile FO, Vulule JM, Slutsker L, 2006. Impact of sustained use of insecticide-treated bednets on malaria vector species distribution and culicine mosquitoes. J Med Entomol 43: 428–432.
  12. Minakawa N, Munga S, Atieli F, Mushinzimana E, Zhou G, Githeko AK, Yan G, 2005. Spatial distribution of anopheline larval habitats in western Kenyan highlands: effects of land cover types and topography. Am J Trop Med Hyg 73: 157–165.
  13. Explorer P, 2005. PrimerExplorer V.4. Available at: http://primerexplorer.jp/elamp4.0.0/index.html. Accessed June 16, 2009.
  14. Towson HOA, 1994. Identification by rDNA-PCR of Anopheles bwambae, a geothermal spring species of the An. gambaie complex. Insect Mol Biol 3: 279–282.
  15. Banoo S, Bell D, Bossuyt P, Herring A, Mabey D, Poole F, Smith PG, Sriram N, Wongsrichanalai C, Linke R, O’Brien R, Perkins M, Cunningham J, Matsoso P, Nathanson CM, Olliaro P, Peeling RW, Ramsay A, 2006. Evaluation of diagnostic tests for infectious diseases: general principles. Nat Rev Microbiol 4: S20–S32.
  16. Confidence Interval Calculator, 2002. Confidence Interval Calculator V4. Available at: http://vl.academicdirect.org/applied_statistics/binomial_distribution/ref/CIcalculator.xls. Accessed June 16, 2009.
  17. Coetzee M, 2004. Distribution of the African malaria vectors of the Anopheles gambiae complex. Am J Trop Med Hyg 70: 103–104.
  18. Moreno MC, Nzambo S, Bobuasaki L, Buatiche JN, Ondo M, Micha F, Benito A, 2004. Malaria Panel Assay versus PCR: detection of naturally infected Anopheles melas in a coastal village of Equatorial Guinea. Malar J 3: 20–25.
  19. Tsy LD, Marrama L, Rabarison P, Le Goff G, Rajaonarivelo V, Robert V, 2003. Distribution of the species of the Anopheles gambiae complex and first evidence of Anopheles merus as a malaria vector in Madagascar. Malar J 2: 33–39.
  20. White GB, 1985. Anopheles bwambae sp.n., a malaria vector in Semliki Valley, Uganda, and its relationships with other sibling species of the An. gambiae complex. Syst Entomol 10: 501–522.
  21. Temu EA, Coetzee M, Minjas JN, Schiff CJ, 1997. Detection of hybrids in natural populations of the Anopheles gambiae complex by rDNA-based, PCR method. Ann Trop Med Parasitol 8: 963–965.
  22. Poon LL, Ma EH, Chan KH, Chow LM, Abeyewickreme W, Tangpukdee N, Yuen KY, Guan Y, Looareesuwan S, Peiris JS, 2006. Sensitive and inexpensive molecular test for falciparum malaria: detecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplification. Clin Chem 52: 303–306.
  23. Aonuma H, Suzuki M, Iseki H, Perera N, Nelson B, Igarashi I, Yagi T, Kanuka H, Fukumoto S, 2008. Rapid identification of Plasmodium-carrying mosquitoes using loop-mediated isothermal amplification. Biochem Biophys Res Commun 376: 671–676.
  24. Mori Y, Hirano T, Notomi T, 2006. Sequence specific visual detection of LAMP reactions by addition of cationic polymers. BMC Biotechnol 6: 3–12.
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.2009.09-0333
Loading
/content/journals/10.4269/ajtmh.2009.09-0333
Loading

Data & Media loading...

  • Received : 16 Jun 2009
  • Accepted : 08 Sep 2009

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error