1921
Volume 81, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Real-time fluorescence resonance energy transfer (FRET) polymerase chain reaction (PCR) supplemented with melting curve analysis is a highly sensitive and fast method offering a high throughput. We report the development of a real-time FRET PCR for molecular detection of in human fecal samples. The diagnostic sensitivity, specificity, accuracy, and positive and negative predictive values of this method were 97.5%, 100%, 98.9%, 100%, and 98.2%, respectively. The sensitivity was not significantly different from that of the quantified formalin-ethyl acetate concentration technique, the gold standard ( > 0.05). The procedure has potential for diagnosis of human opisthorchiasis in disease-endemic areas, for large epidemiologic investigations involving at risk populations, and monitoring eradication programs of the liver fluke, which causes hepatobiliary diseases and induces cholangiocarcinoma.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.2009.09-0275
2009-11-01
2017-09-24
Loading full text...

Full text loading...

/deliver/fulltext/14761645/81/5/0810917.html?itemId=/content/journals/10.4269/ajtmh.2009.09-0275&mimeType=html&fmt=ahah

References

  1. World Health Organization, 1995. Control of foodborne trematode infections. Report of a WHO Study Group. World Health Organ Tech Rep Ser 849: 1–57.
  2. Chai JY, Darwin Murrell K, Lymbery AJ, 2005. Fish-borne parasitic zoonoses: status and issues. Int J Parasitol 35: 1233–1254.
  3. Yossepowitch O, Gotesman T, Assous M, Marva E, Zimlichman R, Dan M, 2004. Opisthorchiasis from imported raw fish. Emerg Infect Dis 10: 2122–2126.
  4. Woolf A, Green J, Levine JA, Estevez EG, Weatherly N, Rosenberg E, Frothingham T, 1984. A clinical study of Laotian refugees infected with Clonorchis sinensis or Opisthorchis viverrini. Am J Trop Med Hyg 33: 1279–1280.
  5. Schwartz DA, 1986. Cholangiocarcinoma associated with liver fluke infection: a preventable source of morbidity in Asian immigrants. Am J Gastroenterol 81: 76–79.
  6. Peng HW, Chao HL, Fan PC, 1993. Imported Opisthorchis viverrini and parasite infections from Thai labourers in Taiwan. J Helminthol 67: 102–106.
  7. Mairiang E, Haswell-Elkins MR, Mairiang P, Sithithaworn P, Elkins DB, 1993. Reversal of biliary tract abnormalities associated with Opisthorchis viverrini infection following praziquantel treatment. Trans R Soc Trop Med Hyg 87: 194–197.
  8. Schwartz DA, 1980. Helminths in the induction of cancer: Opisthorchis viverrini, Clonorchis sinensis and cholangiocarcinoma. Trop Geogr Med 32: 95–100.
  9. Harinasuta T, Riganti M, Bunnang D, 1984. Opisthorchis viverrini infection: pathogenesis and clinical features. Arzneimittelforschung 34: 1167–1169.
  10. Pungpak S, Chalermrut K, Harinasuta T, Viravan C, Schelp PF, Hempfling A, Schlattmann P, Bunnag D, 1994. Opisthorchis viverrini infection in Thailand: symptoms and signs of infection-a population-based study. Trans R Soc Trop Med Hyg 88: 561–564.
  11. Sripa B, Kaewkes S, Sithithaworn P, Mairiang E, Laha T, Smout M, Pairojkul C, Bhudhisawasdi V, Tesana S, Thinkamrop B, Bethony JM, Loukas A, Brindley PJ, 2007. Liver fluke induces cholangiocarcinoma. PLoS Med 4: 1148–1154.
  12. Wongratanacheewin S, Pumidonming W, Sermswan RW, Maleewong W, 2001. Development of a PCR-based method for the detection of Opisthorchis viverrini in experimentally infected hamsters. Parasitology 122: 175–180.
  13. Wongratanacheewin S, Pumidonming W, Sermswan RW, Pipitgool V, Maleewong W, 2002. Detection of Opisthorchis viverrini in human stool specimens by PCR. J Clin Microbiol 40: 3879–3880.
  14. Stensvold CR, Saijuntha W, Sithithaworn P, Wongratanacheewin S, Strandgaard H, Ornbjerg N, Johansen MV, 2006. Evaluation of PCR based coprodiagnosis of human opisthorchiasis. Acta Trop 97: 26–30.
  15. Duenngai K, Sithithaworn P, Rudrappa UK, Iddya K, Laha T, Stensvold CR, Strandgaard H, Johansen MV, 2008. Improvement of PCR for detection of Opisthorchis viverrini DNA in human stool samples. J Clin Microbiol 46: 366–368.
  16. Umesha KR, Kumar S, Parvathi A, Duenngai K, Sithithaworn P, Karunasagar I, Karunasagar I, 2008. Opisthorchis viverrini: detection by polymerase chain reaction (PCR) in human stool samples. Exp Parasitol 120: 353–356.
  17. Intapan PM, Thanchomnang T, Lulitanond V, Pongsaskulchoti P, Maleewong W, 2008. Detection of Opisthorchis viverrini in infected bithynid snails by real-time fluorescence resonance energy transfer PCR-based method and melting curve analysis. Parasitol Res 103: 649–655.
  18. Intapan PM, Thanchomnang T, Lulitanond V, Phongsaskulchoti P, Maleewong W, 2008. Real-time fluorescence resonance energy transfer PCR with melting curve analysis for the detection of Opisthorchis viverrini in fish intermediate hosts. Vet Parasitol 157: 65–71.
  19. Suksumek N, Leelawat K, Leelawat S, Russell B, Lek-Uthai U, 2008. TaqMan real-time PCR assay for specific detection of Opisthorchis viverrini DNA in Thai patients with hepatocellular carcinoma and cholangiocarcinoma. Exp Parasitol 119: 217–224.
  20. Lyon E, Wittwer CT, 2009. LightCycler technology in molecular diagnostics. J Mol Diagn 11: 93–101.
  21. Elkins DB, Haswell-Elkins M, Anderson RM, 1986. The epidemiology and control of intestinal helminths in the Pulicat Lake region of southern India. I. Study design and pre- and post-treatment observations on Ascaris lumbricoides infection. Trans R Soc Trop Med Hyg 80: 774–792.
  22. Sermswan R, Mongkolsuk S, Panyim S, Sirisinha S, 1991. Isolation and characterization of Opisthorchis viverrini specific DNA probe. Mol Cell Probes 5: 399–407.
  23. Thanchomnang T, Intapan PM, Lulitanond V, Choochote W, Manjai A, Prasongdee TK, Maleewong W, 2008. Rapid detection of Brugia malayi in mosquito vectors using a real-time fluorescence resonance energy transfer PCR and melting curve analysis. Am J Trop Med Hyg 78: 509–513.
  24. Intapan PM, Maleewong W, 2006. Opisthorchis viverrini: influence of maternal infection in hamsters on offspring infected with homologous parasite and their IgG antibody response. Exp Parasitol 113: 67–74.
  25. Galen RS, 1980. Predictive value and efficiency of laboratory testing. Pediatr Clin North Am 27: 861–869.
  26. Sirisinha S, Chawengkirttikul R, Haswell-Elkins MR, Elkins DB, Kaewkes S, Sithithaworn P, 1995. Evaluation of a monoclonal antibody-based enzyme linked immunosorbent assay for the diagnosis of Opisthorchis viverrini infection in an endemic area. Am J Trop Med Hyg 52: 521–524.
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.2009.09-0275
Loading
/content/journals/10.4269/ajtmh.2009.09-0275
Loading

Data & Media loading...

  • Received : 23 May 2009
  • Accepted : 15 Jul 2009

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error