1921
Volume 81, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

We have applied a loop-mediated isothermal amplification (LAMP) technique to detect DNA. The LAMP technique detected 1 fg of DNA, which was 10-fold more sensitive than a conventional polymerase chain reaction (PCR). All nested PCR–positive blood samples from visceral leishmaniasis patients were positive with the LAMP technique, and DNA samples from , , , , , , and healthy humans were negative with the LAMP technique. The advantages of the LAMP method are its shorter reaction time, a lack of requirement of sophisticated equipment, and visual judgment of positivity based on the turbidity of reaction mixture. Our LAMP technique can be a better alternative to a conventional PCR, especially under field conditions.

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2009-10-01
2017-09-22
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  • Received : 18 Mar 2009
  • Accepted : 17 Jun 2009

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