Volume 81, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


The prevalence, host preference, and rate of spp. infection of sand fly species are important parameters for incrimination of parasite vectors. We applied polymerase chain reaction (PCR)-based and enzyme-linked immunosorbent assay (ELISA) methods to detect spp. parasites and blood meals within individual sand flies in the most important visceral leishmaniasis (VL) focus in northwestern Iran. spp. minicircles (kinetoplast DNA) were found in 14 (0.9%) of 1,569 female specimens. Sequence analysis of 650 basepairs of an internal transcribed spacer ribosomal DNA gene identified in 12 specimens and -like parasites in 2 specimens. Nine (64.3%) of 14 of the spp.–positive sand flies were . Blood meal identification of host DNA within sand flies by PCR-based and ELISA methods showed that 30% and 28%, respectively, were positive for human blood. Results of this study showed that is the most prevalent, infected, and anthropophagic sand fly and plays a major role in VL transmission in the region studied.


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  • Received : 14 Sep 2008
  • Accepted : 28 Dec 2008

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