Volume 79, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


A real-time quantitative polymerase chain reaction (qPCR) assay was developed for . The qPCR assay was developed utilizing a conserved region of the ferric iron uptake regulator gene () to design primers and a fluorescent (FAM-labeled) Man probe. The assay was optimized using cultured (UG05-0454) and was confirmed to work with strains from 3 biovars. The optimized assay was capable of detecting a single organism of cultured and as little as 300 bacteria in infected flea triturates. This qPCR assay enables rapid enumeration of bacterium in laboratory-infected fleas when compared with conventional serial dilution plating.


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  • Received : 09 Jan 2008
  • Accepted : 25 Mar 2008

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