1921
Volume 79, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

In this study 2,089 fecal samples from patients with gastroenteritis were analyzed from different hospitals in Panama, Costa Rica, and the Dominican Republic during the period comprised between December 2002 and July 2003. One hundred samples per country from the positives to the enzyme-linked immunosorbent assay (ELISA) kit were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) to determine the G and P genotypes: in Panama, Costa Rica, and Dominican Republic the combinations G and P have a great diversity and unusual genotypes. These results highlight an unexpected diversity among rotavirus strains in these countries and emphasize the need for further serologic and genetic surveys on more rotavirus strains in Central America and the Caribbean. In this context, the next generation of rotavirus vaccines will need to provide adequate protection against diseases caused by unusual genotypes. These results represent the second report of rotavirus genotypes in Costa Rica and first-time reports of rotavirus genotypes in Panama and the Dominican Republic.

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2008-12-01
2017-09-25
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References

  1. Parashar UD, Hummelman EG, Bresee JS, Miller MA, Glass RI, 2003. Global illness and deaths caused rotavirus disease in children. Emerg Infect Dis 9 : 565–572.
  2. Hoshino Y, Kapikian A, 2000. Rotavirus serotypes: classification and importance in rotavirus epidemiology, immunity and vaccine development. J Health Popul Nutr 18 : 5–14.
  3. Kapikian A, Hoshino Y, Chanock R, 2001. Rotaviruses. Knipe DM, Howley RM, eds., Fields Virology. Fourth edition. Philadelphia. Lippincott, Williams and Wilkins, 1787–1825.
  4. Martella VM, Ciarlet K, Banyai E, Lorusso A, Cavalli M, Corrente G, Elia S, Arista M, Camero C, Desario N, Decaro A, Lavazza A, Buonavoglia C, 2006. Identification of a novel VP4 genotype carried by a serotype G5 porcine rotavirus strain. Virology 346 : 301–311.
  5. Santos N, Hoshino Y, 2005. Global distribution of rotavirus serotypes/genotypes and its implication for the development and implementation of an effective rotavirus vaccine. Rev Med Virol 15 : 29–56.
  6. Cataloluk O, Iturriza M, Gray J, 2005. Molecular characterization of rotaviruses circulating in the population in Turkey. Epidemiol Infect 133 : 673–678.
  7. Lee CN, Lin CC, Kao CL, Zao CL, Shih MC, Chen HN, 2001. Genetic characterization of the rotaviruses associated with a nursery outbreak. J Med Virol 63 : 311–320.
  8. Santos N, Soares CC, Volotão EM, Albuquerque MC, Hoshino Y, 2003. Surveillance of rotavirus strains in Rio de Janeiro, Brazil, from 1997 to 1999. J Clin Microbiol 41 : 3399–3402.
  9. Abdel-Haq NM, Thomas R, Asmar B, Zacharova V, Lyman WD, 2003. Increased prevalence of G1P[4] genotype among children with rotavirus-associated gastroenteritis in metropolitan Detroit. J Clin Microbiol 41 : 2680–2682.
  10. Arguelles MH, Villegas GA, Castello A, Abrami A, Ghiringhelli PD, Semorile L, Glikmann G, 2000. VP7 and VP4 genotyping of human group A rotavirus in Buenos Aires, Argentina. J Clin Microbiol 38 : 252–259.
  11. Das S, Sen A, Uma G, Varghese V, Chaudhuri S, Bhattacharya SK, Krishnan T, Dutta P, Dutta D, Bhattacharya MK, Mitra U, Kobayashi N, Naik TN, 2002. Genomic diversity of group A rotavirus strains infecting humans in eastern India. J Clin Microbiol 40 : 146–149.
  12. Ramachandran M, Bimalk K, Das K, Vij AA, Ramesh K, Bhambal SS, Kesari N, Rawat H, Bahl L, Thakur S, Woods P, Glass R, Bhan MK, Gentsch J, 1996. Unusual diversity of human rotavirus G and P genotypes in India. J Clin Microbiol 34 : 436–439.
  13. Araújo IT, Ferreira MSR, Fialho AM, Assis RM, Cruz CM, Rocha M, Leite JPG, 2001. Rotavirus genotypes P[4] G9, P[6]G9, and P[8]G9 in hospitalized children with acute gastro-enteritis in Rio de Janeiro, Brazil. J Clin Microbiol 39 : 1999–2001.
  14. Arista S, Giammanco GM, De Grazia S, Migliore MC, Martella V, Cascio A, 2004. Molecular characterization of the genotype G9 human rotavirus strains recovered in Palermo, Italy, during the winter of 1999–2000. Epidemiol Infect 132 : 343–349.
  15. Armah GE, Steele AD, Binka FN, Esona MD, Asmah RH, Anto F, Brown D, Green J, Cutts F, Hall A, 2003. Changing patterns of rotavirus genotypes in Ghana: emergence of human rotavirus G9 as a major cause of diarrhea in children. J Clin Microbiol 41 : 2317–2322.
  16. Carvalho-Costa FA, Assis RM, Fialho AM, Boia MN, Dias DP, Miranda CM, Leite JPG, 2006. Detection and molecular characterization of group A rotavirus from hospitalized children in Rio de Janeiro, Brazil, 2004. Mem Inst Oswaldo Cruz 101 : 291–294.
  17. Cunliffe NA, Dove W, Bunn JEG, Ramadam MB, Nyangao JWO, Riveron RL, Cuevas LE, Hart CA, 2001b. Expanding global distribution of rotavirus serotype G9: detection in Libya, Kenya and Cuba. Emerg Infect Dis 7 : 890–892.
  18. Santos N, Volotao EM, Soares CC, Albuquerque MC, da Silva FM, de Carvalho TR, Pareira CF, Chizhikov V, Hoshino Y, 2001. Rotavirus strains bearing genotype G9 or P9 recovered from Brazilian children with diarrhea from 1997 to 1999. J Clin Microbiol 39 : 1157–1160.
  19. Trabelsi A, Peenze I, Pager C, Jeddi M, Steele D, 2000. Distribution of rotavirus VP7 serotypes and VP4 genotypes circulating in Sousse, Tunisia, from 1995 to 1999: emergence of natural human reassortants. J Clin Microbiol 38 : 3415–3419.
  20. WHO Department of Vaccines and Biologicals. Report of the meeting on future directions for rotavirus vaccine research in developing countries. Document WHO/V&B/00.23. Geneva: World Health Organization, 9–11 February 2000.
  21. Gentsch JR, Glass R, Woods P, Gouvea V, Gorziglia M, Flores J, Das B, Bhan M, 1992. Identification of group A rotavirus gene 4 types by polymerase chain reaction. J Clin Microbiol 30 : 1365–1373.
  22. Gouvea V, Glass P, Woods K, Taniguchi HF, Clark B, Forrester B, Fang ZY, 1990. Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens. J Clin Microbiol 28 : 276–282.
  23. Iturriza-Gómara M, Kang G, Gray J, 2004. Rotavirus genotyping: keeping up with an evolving population of human rotaviruses. J Clin Virol 31 : 259–265.
  24. Maunula L, Bonsdorff C, 2002. Frequent reassortment may explain the genetic heterogeneity of rotavirus: analysis of Finnish rotavirus strain. J Virol Clin 76 : 1793–1800.
  25. O’Halloran F, Fanning S, 2004. Molecular genotyping of Irish rotavirus strains. Methods Mol Biol 268 : 89–102.
  26. Asmah RH, Green J, Armah G, Gallimore C, Gray J, Iturriza-Gomara M, Anto F, Oduro A, Binka F, Brown D, Cutt F, 2001. Rotavirus G and P genotypes in rural Ghana. J Clin Microbiol 39 : 1981–1984.
  27. Bucardo F, Karlsson B, Nordgren J, Paniagua M, González A, Amador J, Espinoza F, Svensson L, 2007. Mutated G4P[8] rotavirus associated with a nationwide outbreak of gastroenteritis in Nicaragua in 2005. J Clin Microbiol 45 : 990–997.
  28. Adah M, Rohwedder I, Olaleye O, Durojaiye O, Werchau H, 1997. Further characterization of field strains of rotavirus from Nigeria VP4 genotype P6 most frequently identified among symptomatically infected children. J Trop Pediatr 43 : 267–274.
  29. Cunliffe N, Gondwe J, Graham S, Thindwa B, Dove W, Broad-head R, Molyneux M, Hart C, 2001a. Rotavirus strain diversity in Blantyre, Malawi, from 1997 to 1999. J Clin Microbiol 39 : 836–843.
  30. Doan LT, Okitsu S, Nishio O, Pham DT, Nguyen DH, Ushijima H, 2003. Epidemiological features of rotavirus infection among hospitalized children with gastroenteristis in Ho Chi Minh City, Vietnam. J Med Virol 69 : 588–594.
  31. Espul C, Cuello H, Martinez N, Centorbi O, O’Ryan M, Jackson L, Campos F, Matson DO, 2000. Genomic and antigenic variation among rotavirus strains circulating in a large city of Argentina. J Med Virol 61 : 504–509.
  32. Griffin DD, Kirkwood CD, Parashar UD, Woods PA, Bresee JS, Glass RI, Gentsch JR, 2000. Surveillance of rotavirus strains in the United States: identification of unusual strains. J Clin Microbiol 38 : 2784–2787.
  33. Iturriza-Gómara M, Green J, Brown D, Ramsay M, Desselberger U, Gray J, 2000. Molecular epidemiology of human group A rotavirus infections in the United Kingdom between 1995 and 1998. J Clin Microbiol 38 : 4394–4401.
  34. Kang G, Green J, Gallimore CI, Brown DW, 2002. Molecular epidemiology of rotaviral infection in South Indian children with acute diarrhea from 1995–1996 to 1998–1999. J Med Virol 67 : 101–105.
  35. O’Halloran F, Lynch M, Cryan B, O’Shea H, Fanning S, 2000. Molecular characterization of rotavirus in Ireland: detection of novel strains circulating in the population. J Clin Microbiol 38 : 3370–3374.
  36. Ramachandran M, Gentsch JR, Parashar UD, Jin S, Woods PA, Holmes JL, Kirkwood CD, Bishop RF, Greenberg HB, Urasawa G, Gerna B, Coulson BS, Taniguchi K, Bresee JS, Glass RI, 1998. Detection and characterization of novel rotavirus strains in the United States. J Clin Microbiol 36 : 3223–3229.
  37. Urbina D, Rodríguez JG, Arzuza O, Parra E, Young G, Castro R, Portillo P, 2004. G and P genotypes of rotavirus circulating among children with diarrhea in the Colombian northern coast. Int Microbiol 7 : 113–120.
  38. Banerjee I, Ramani S, Primrose B, Moses P, Iturriza-Gomara M, Gray J, Jaffar S, Monica B, Muliyil J, Brown D, Estes M, Kang G, 2006. Comparative study of the epidemiology of rotavirus in children from a community-based birth cohort and a hospital in South India. J Clin Microbiol 44 : 2468–2474.
  39. Armah GE, Pager CT, Asmah RH, Anto FR, Oduro AR, Binka F, Steele AD, 2001. Prevalence of unusual human rotavirus strains in Ghanaian children. J Med Virol 63 : 67–71.
  40. Coluchi N, Munford V, Manzur J, Vazquez C, Escobar M, Weber E, Mármol P, Ráez L, 2002. Detection subgroup specificity and genotype diversity of rotavirus strains in children with acute diarrhea in Paraguay. J Clin Microbiol 40 : 1709–1714.
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  • Received : 23 May 2007
  • Accepted : 19 Sep 2008

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