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Volume 79, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Visceral leishmaniasis (VL), caused by , is endemic in southern Iran. To detect asymptomatic individuals, we used kinetoplast DNA (kDNA) polymerase chain reaction (PCR)-ELISA methods on 388 blood samples of healthy persons in two endemic loci and compared the results with the leishmanin skin test (LST) and the immunofluorescent antibody test (IFAT). kDNA PCR, LST, and IFAT were positive in 95 (24.5%), 132 (34%), and 212 (54.6%) cases, respectively. Fifty-five (21.4%) individuals that were LST negative were PCR positive. All PCR-positive individuals had a titer of ≥ 1:20, whereas 45% of those that were IFAT positive were PCR positive. For a reliable index of prevalence rate of infection, LST alone is not sufficient and needs to be accompanied by PCR-ELISA. The high rate of kDNA-positive results may indicate the possibility of humans being a reservoir and source of transmission. In endemic areas, kDNA PCR-ELISA is not a reliable test for the diagnosis of active VL.

Copyright 2008 The American Society of Tropical Medicine
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/content/journals/10.4269/ajtmh.2008.79.839
2008-12-01
2018-08-17

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References

  1. Alborzi A, Rasouli M, Nademi Z, Kadivar MR, Pourabbas B, 2006. Evaluation of rK39 strip test for the diagnosis of visceral leishmaniasis in infants. East Mediterr Health J 12 : 294–299.
  2. Alborzi A, Torab Jahromi F, 1993. Autoantibodies and complement level of kala-azar patients in the south of Iran. Iran. J Med Sci 18 : 94–98.
  3. le Fichoux Y, Quaranta JF, Aufeuvre JP, Lelievre A, Marty P, Suffia I, Rousseau D, Kubar J, 1999. Occurrence of Leishmania infantum parasitemia in asymptomatic blood donors living in an area of endemicity in southern France. J Clin Microbiol 37 : 1953–1957.
  4. Martin-Sanchez J, Pineda JA, Morillas-Marquez F, Garcia-Garcia JA, Acedo C, Macias J, 2004. Detection of Leishmania infantum kinetoplast DNA in peripheral blood from asymptomatic individuals at risk for parenterally transmitted infections: relationship between polymerase chain reaction results and other Leishmania infection markers. Am J Trop Med Hyg 70 : 545–548.
  5. Costa CH, Stewart JM, Gomes RB, Garcez LM, Ramos PK, Bozza M, Satoskar A, Dissanayake S, Santos RS, Silva MR, Shaw JJ, David JR, Maguire JH, 2002. Asymptomatic human carriers of Leishmania chagasi. Am J Trop Med Hyg 66 : 334–337.
  6. Rieraa C, Fisa R, Udinab M, Gallegoa M, Portusa M, 2004. Detection of Leishmania infantum cryptic infection in asymptomatic blood donors living in an endemic area (Eivissa, Balearic Islands, Spain) by different diagnostic methods. Trans R Soc Trop Med Hyg 98 : 102–110.
  7. Rodgers MR, Popper SJ, Wirth DF, 1990. Amplification of kine-toplast as a tool in the detection and diagnosis of Leishmania. Exp Parasitol 71 : 267–275.
  8. Aviles H, Belli A, Armijos R, Monroy FP, Harris E, 1999. PCR detection and identification of Leishmania parasites in clinical specimens in Ecuador: a comparison with classical diagnostic methods. J Parasitol 85 : 181–187.
  9. Smyth AJ, Ghosh A, Hassan MQ, Basu D, De Bruijn MH, Adhya S, Mallik KK, Barker DC, 1992. Rapid and sensitive detection of Leishmania kinetoplast DNA from spleen and blood samples of kala-azar patients. Parasitology 105 : 183–192.
  10. Antinori S, Calattini S, Longhi E, Bestetti G, Piolini R, Magni C, Orlando G, Gramiccia M, Acquaviva V, Foschi A, Corvasce S, Colomba C, Titone L, Parravicini C, Cascio A, Corbellino M, 2007. Clinical use of polymerase chain reaction performed on peripheral blood and bone marrow samples for the diagnosis and monitoring of visceral leishmaniasis in HIV-infected and HIV-uninfected patients: a single-center, 8-year experience in Italy and review of the literature. Clin Infect Dis 44 : 1602–1610.
  11. Martin-Sánchez J, Lopez-Lopez MC, Acedo-Sánchez C, Castro-Fajardo JJ, Pineda JA, Morillas-Márquez F, 2001. Diagnosis of infections with Leishmania infantum using PCR-ELISA. Parasitology 122 : 607–615.
  12. Lachaud L, Chabbert E, Dubessay P, Reynes J, Lamothe J, Bastien P, 2001. Comparison of various sample preparation methods for PCR diagnosis of visceral leishmaniasis using peripheral blood. J Clin Microbiol 39 : 613–617.
  13. Badaro R, Jones TC, Carvalho JM, Sampaio D, Reed SG, Barral A, Teixeira R, Johnson WD, 1986. New perspectives on a subclinical form of visceral leishmaniasis. J Infect Dis 154 : 1003–1011.
  14. Pearson RD, de Queiroz Sousa A, 1995. Clinical spectrum of leishmaniasis. Clin Infect Dis 22 : 1–13.
  15. Badaro R, Reed SG, Carvalho EM, 1983. Immunofluorescent antibody test in American visceral leishmaniasis: sensitivity and specificity of different morphological forms of two Leishmania species. Am J Trop Med Hyg 32 : 480–484.
  16. Marty P, Lelievre A, Quaranta JF, Rahal A, Gari-Thoussaint M, Le Fichoux Y, 1994. Use of the leishmanin skin test and western blot analysis for epidemiological studies in visceral leishmaniasis areas: experience in a highly endemic focus in Alpesmaritimes (France). Trans R Soc Trop Med Hyg 88 : 658–659.
  17. Liew FY, O’Donnell A, 1993. Immunology of leishmaniasis. Adv Parasitol 32 : 161–259.
  18. Martín-Sánchez J, Pineda JA, Andreu-López M, Delgado J, Macías J, de la Rosa R, Morillas-Márquez F, 2002. The high sensitivity of a PCR-ELISA in the diagnosis of cutaneous and visceral leishmaniasis caused by Leishmania infantum. Ann Trop Med Parasitol 96 : 669–677.
  19. Mary C, Faraut F, Drogoul MP, Xeridat B, Schleinitz N, Cuisenier B, Dumon H, 2006. Reference values for Leishmania infantum parasitemia in different clinical presentations: quantitative polymerase chain reaction for therapeutic monitoring and patient follow-up. Am J Trop Med Hyg 75 : 858–863.
  20. Bogdan C, Schönian G, Bañuls AL, Hide M, Pratlong F, Lorenz E, Röllinghoff M, Mertens R, 2001. Visceral leishmaniasis in a German child who had never entered a known endemic area: case report and review of the literature. Clin Infect Dis 32 : 302–306.
  21. Pintado V, Martin-Rabadan P, Rivera M, Moreno S, Bouza E, 2001. Visceral leishmaniasis in human immunodeficiency virus (HIV)-infected and non-HIV-infected patients: a comparative study. Medicine 80 : 54–73.
  22. Alvar J, Canavate C, Gutierrez-Solar B, Jime’nez M, Laguna F, Lopez-Ve’lez R, Molina R, Moreno J, 1997. Leishmania and human immunodeficiency virus coinfection: the first 10 years. Clin Microbiol Rev 10 : 298–319.
  23. Acedo Sánchez C, Martín Sánchez J, Vélez Bernal ID, Sanchís Marín MC, Louassini M, Maldonado JA, Morillas Márquez F, 1996. Leishmaniasis eco-epidemiology in the Alpujarra region (Granada Province, southern Spain). Int J Parasitol 26 : 303–310.
  24. Gramiccia M, Bettini S, Gradoni L, Ciarmoli P, Verrelli ML, Loddo S, Cicalo C, 1990. Leishmaniasis in Sardinia. Leishmanin reaction in the human population of a focus of low endemicity of canine leishmaniasis. Trans R Soc Trop Med Hyg 84 : 371–374.
  25. Moral L, Rubio EM, Moya M, 2002. A leishmanin skin test survey in the human population of l’Alacantí Region (Spain): implications for the epidemiology of Leishmania infantum infection in southern Europe. Trans R Soc Trop Med Hyg 96 : 129–132.
  26. De Almeida MC, Cardoso SA, Barral-Netto M, 2003. Leishmania (Leishmania) chagasi infection alters the expression of cell adhesion and costimulatory molecules on human monocyte and macrophage. Int J Parasitol 33 : 153–162.
  27. Charalabides S, Dafas G, Epivatianos P, 1992. Detection of specific IgG, IgM and IgE immunoglobulins towards various parasites in the population of Macedonia. Acta Microbiol Hellenica 37 : 365–367.
  28. Papadopoulou C, Dimitriou D, Panagiou A, Manis E, Antoniades G, 1995. Canine leishmaniasis in Greece: a survey in Northwestern Greece. Acta Microbiol Hellenica 40 : 54–57.
  29. Kohanteb J, Ardehali S, 2005. Cross-reaction of sera from patients with various infectious diseases with Leishmania infantum. Med Princ Pract 14 : 79–82.
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Detection of Leishmania infantum Kinetoplast DNA in the Whole Blood of Asymptomatic Individuals by PCR-ELISA and Comparison with Other Infection Markers in Endemic Areas, Southern Iran
The American Journal of Tropical Medicine and Hygiene 79, 839 (2008); https://doi.org/10.4269/ajtmh.2008.79.839
/content/journals/10.4269/ajtmh.2008.79.839
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  • Received : 18 Feb 2008
  • Accepted : 08 Aug 2008

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