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Volume 79, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Leptospirosis, caused by spp., is a zoonotic disease found worldwide. Killed whole cell leptospiral vaccines have been used as effective vaccines to elicit specific antibodies for protection. However, the involvement of cytokine responses after vaccination is not well characterized. Hamsters were immunized with killed serovar Autumnalis before challenge to study cytokine mRNA expression levels (interferon [IFN]-γ, tumor necrosis factor [TNF]-α, interleukin [IL]-10, and IL-4). Vaccinated groups showed 92–100% survival rates, whereas control hamsters died within 6–10 days. However, live organisms were detected in vaccinated groups, and mild to moderate pathology was observed early in infection. IFN-γ and TNF-α mRNA expression levels correlated with the severity of infection and lung pathology, whereas IL-4 and IL-10 expression levels were significantly higher in vaccinated groups. In summary, commonly used vaccines changed the cytokine profiles and protected hamsters from death but failed to stimulate sterile immunity and were unable to prevent the occurrence of pathology.

Copyright 2008 The American Society of Tropical Medicine
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2008-11-01
2018-10-17

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Analyses of Vaccination Protocols for Leptospira interrogans Serovar Autumnalis in Hamsters
The American Journal of Tropical Medicine and Hygiene 79, 779 (2008); https://doi.org/10.4269/ajtmh.2008.79.779
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  • Received : 12 May 2008
  • Accepted : 13 Aug 2008

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