Volume 79, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Sand flies from the Andean areas of Ecuador and Peru were examined for infections by using our recently established molecular mass screening method. Leishmanial minicircle DNA-positive sand flies were detected in 3 of 192 and 1 of 462 samples from Ecuador and Peru, respectively. Sand fly species were identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the 18S ribosomal RNA (rRNA) gene, and the positive flies were () and respectively. Furthermore, cytochrome and mannose-phosphate isomerase gene sequence analyses identified the parasites from Ecuador and Peru as () and () respectivel. Thus, the mass screening method was confirmed to be a powerful tool for sand fly research.


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  • Received : 05 Feb 2008
  • Accepted : 12 Jul 2008

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