Volume 79, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Prevalence and species distribution of spp. were determined among 633 immunocompetent children less than five years of age and 75 patients hospitalized for immunodeficiency who lived in northern Tunisia. Microscopy was used for initial screening to detect positive samples and a nested polymerase chain reaction and restriction fragment length polymorphism analysis was used to determine the species. spp. was identified in 2.7% of cases (19 stool samples), and there was a significant difference between samples collected from immunocompromised patients and those collected from healthy children (10.7% versus 1.7%). Prevalence was also significantly higher in diarrheal specimens than in formed specimens (6.3% versus 1.6%). and were responsible for most spp. infections (78.9%). was more prevalent in children from urban areas than in those from rural areas, and was found with similar prevalence rates in the two populations. was identified in four children on farms.


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  1. Tzipori S, Ward H, 2002. Cryptosporidiosis: biology, pathogenesis and disease. Microbes Infect 4 : 1047–1058. [Google Scholar]
  2. Huang DB, Chappell C, Okhuysen PC, 2004. Cryptosporidiosis in children. Semin Pediatr Infect Dis 15 : 253–259. [Google Scholar]
  3. Corso PS, Kramer MH, Blair KA, Addiss DG, Davis JP, Haddix JP, 2003. Cost of illness in the 1993 waterborne Cryptosporidium outbreak, Milwaukee, Wisconsin. Emerg Infect Dis 9 : 426–431. [Google Scholar]
  4. Xiao L, Fayer R, Ryan U, Upton SJ, 2004. Cryptosporidium taxonomy: recent advances and implications for public health. Clin Microbiol Rev 17 : 72–97. [Google Scholar]
  5. Belhadj S, Kallel K, Boussen N, Goubantini A, Bejaoui M, Ben Salem N, Zribi A, Ben Chaabane T, Chaker E, 1999. Role of cryptosporidia and microsporidia in diarrhea in immunocompromised patients. Tunis Med 77 : 638–643. [Google Scholar]
  6. Bouratbine A, Aoun K, Barbouche R, Bejaoui M, Ben Ismaïl R, 1998. Fréquence d’isolement des oocystes de Cryptosporidium dans les selles diarrhéiques d’enfants tunisiens. Med Mal Infect 28 : 308–310. [Google Scholar]
  7. Fathallah A, Saghrouni F, Madani B, Ben Rejeb N, Ben Said M, 2004. La cryptosporidiose dans la region de Sousse, Tunisie. Arch Pediatr 11 : 207–211. [Google Scholar]
  8. Casemore DP, Amstrong M, Jackson B, 1984. Screening for Cryptosporidium in stools. Lancet I : 734–735. [Google Scholar]
  9. Coupe S, Sarfati C, Hamame S, Derouin F, 2005. Detection of Cryptosporidium and identification to the species level by nested PCR and restriction fragment length polymorphism. J Clin Microbiol 43 : 1017–1023. [Google Scholar]
  10. Llorente MT, Clavel A, Goni MP, Varea M, Seral C, Becerril R, Suarez L, Gomez-Lus R, 2007. Genetic characterization of Cryptosporidium species from humans in Spain. Int J Parasitol 56 : 201–205. [Google Scholar]
  11. Gatei W, Suputtamongkol Y, Waywa D, Ashford RW, Bailey JW, Greensill J, Beeching NJ, Hart CA, 2002. Zoonotic species of Cryptosporidium are as prevalent as the anthroponotic in HIV-infected patients in Thailand. Ann Trop Med Parasitol 96 : 797–802. [Google Scholar]
  12. Xiao L, Bern C, Limor J, Sulaiman I, Roberts J, Checkley W, Cabrera L, Gilman RH, Lal AA, 2001. Identification of 5 types of Cryptosporidium parasites in children in Lima, Peru. J Infect Dis 183 : 492–497. [Google Scholar]
  13. Caccio SM, 2005. Molecular epidemiology of human cryptosporidiosis. Parasitologia 47 : 185–192. [Google Scholar]
  14. Learmonth JJ, Ionas G, Ebbett KA, Kwan ES, 2004. Genetic characterization and transmission cycles of Cryptosporidium species isolated from humans in New Zealand. Appl Environ Microbiol 70 : 3973–3978. [Google Scholar]
  15. Alves M, Xiao L, Antunes F, Matos O, 2006. Distribution of Cryptosporidium subtypes in humans and domestic and wild ruminants in Portugal. Parasitol Res 99 : 287–292. [Google Scholar]

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  • Received : 28 Jan 2008
  • Accepted : 20 Jun 2008

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