Volume 79, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Oxidative stress in dengue infection has been suggested. This study was carried out to explore the plasma protein oxidation and its sialic acid content in dengue infection. Thirty-two dengue hemorrhagic fever (DHF), 25 dengue shock syndrome (DSS), 29 dengue fever (DF), and 63 healthy controls were included in this study. The extent of carbonylation, sulphydryl content, and desialylation of plasma protein was estimated in acute phase sample. Significantly higher levels of protein carbonyls and lower levels of sialic acid and sulphydryl groups were found in DHF and DSS compared with DF using one-way analysis of variance. Regression analysis showed that desialylation is dependent on protein carbonyls in DHF/DSS. This study indicates that, in dengue infection, plasma proteins undergo increased levels of desialylation, which can be attributed to the oxidative stress. Future studies on sialylation status of endothelium and platelets can show light into the pathogenesis of the dengue infection.


Article metrics loading...

Loading full text...

Full text loading...



  1. Gubler DJ, 1998. Dengue and dengue hemorrhagic fever. Clin Microbiol Rev 11 : 480–496.
  2. Gibbons RV, Vaughn DW, 2002. Dengue: an escalating problem. BMJ 324 : 1563–1566.
  3. Taniuchi K, Chifu K, Hayashi N, Nakamachi Y, Yamaguchi N, Miyamato Y, 1981. A new enzymatic method for the determination of sialic acid and its application as a marker of acute phase reactants. Kobe J Med Sci 27 : 91–102.
  4. Petren S, 1989. Vesterberg 0. The N-acetylneuraminic acid content of five forms of human transferrin. Biochim Biophys Acta 994 : 161–165.
  5. Nurden AT, George JN, Philips DR, 1986. Platelet membrane glycoproteins: their structure, function, and modification in disease. Philips DR, Shuman MA, eds. Biochemistry of Platelets. Orlando, FL: Academic Press, 160–212.
  6. Thornhill WB, Wu MB, Jiang X, Wu X, Morgan PT, Margiotta JF, 1996. Expression of Kv1.1 delayed rectifier potassium channels in Lec mutant Chinese hamster ovary cell lines reveals a role for sialidation in channel function. J Biol Chem 271 : 19093–19098.
  7. Murray RK, 1996. Glycoproteins. Murray RK, Granner DK, Mayes PA, Rodwell VW, eds. Harper’s Biochemistry. 24th edition. NJ: Prentice Hall, 651.
  8. Goswami K, Nandakumar DN, Koner BC, Bobby Z, Sen SK, 2003. Oxidative changes and desialylation of serum proteins in hyperthyroidism. Clin Chim Acta 337 : 163–168.
  9. Halstead SB, 1988. Pathogenesis of dengue. Challenges to molecular biology. Science 239 : 476–481.
  10. Kurane I, Takasaki T, 2001. Dengue fever and dengue haemorrhagic fever: challenges of controlling an enemy still at large. Rev Med Virol 11 : 301–311.
  11. Chatuverdi UC, Agarwal R, Elbishbishi EA, Mustafa AS, 2000. Cytokine cascade in dengue hemorrhagic fever: implications for pathogenesis. FEMS Immunol Med Microbiol 28 : 183–188.
  12. Mongkolsapaya J, Dejnirattisai W, Xu X, Vasanawathana S, Tangthawornchaikul N, Chairunsri A, Sawasdivorn S, Duangchinda T, Dong T, Rowland-Jones S, Yenchitsomanus P, McMichael A, Malasit P, Screaton G, 2003. Original antigenic sin and apoptosis in the pathogenesis of dengue hemorrhagic fever. Nat Med 9 : 921–927.
  13. Gil L, Martinez G, Tapanes R, Castro O, González D, Bernardo L, Vázquez S, Kourí G, Guzman M, 2004. Oxidative stress in adult dengue patients. Am J Trop Med Hyg 71 : 652–657.
  14. Peterhans E, Grob M, Burge T, Zanoni R, 1987. Virus-induced formation of reactive oxygen intermediates in phagocytic cells. Free Radic Res Commun 3 : 39–46.
  15. Sahnoun Z, Jamoussi K, Zeghal KM, 1997. Free radicals and antioxidants: human physiology, pathology and therapeutics aspects. Therapie 52 : 251–270.
  16. Yoshikawa T, 1996. Vascular dysfunction and free radicals. Free Radic Biol Med 2002; 33 : 425–426.
  17. Winter ML, Liehr JG, 1991. Free radical-induced carbonyl content in protein of estrogen-treated hamsters assayed by sodium boro [3H] hydride reduction. J Biol Chem 266 : 14446–14450.
  18. Wolff SP, Dean R, 1986. Fragmentation of protein by free radicals and its effect on their susceptibility to enzymic hydrolysis. Biochem J 234 : 399–403.
  19. Pantke U, Volk T, Schmutzler M, Kox WJ, Sitte N, Grune T, 1999. Oxidized proteins as a marker of oxidative stress during coronary heart surgery. Free Radic Biol Med 27 : 1080–1086.
  20. Wayner DDM, Burton GW, Ingold KU, Barclay LRC, Locke SJ, 1987. The relative contributions of vitamin E, urate, ascorbate, and proteins to the total peroxyl radical trapping antioxidant activity of human plasma. Biochim Biophys Acta 924 : 408–419.
  21. Hoti SL, Soundravally R, Rajendran G, Das LK, Ravi R, Das PK, 2006. Dengue and dengue haemorrhagic fever outbreaks in Pondicherry, South India, during 2003–2004: emergence of DENV-3. Dengue Bull 30 : 42–50.
  22. World Health Organization, 1997. Dengue Haemorrhagic Fever: Diagnosis, Treatment, Prevention, and Control. Second edition. Geneva: World Health Organization.
  23. Lanciotti RS, Calisher CH, Gubler DJ, Chang G-J, Vorndam V, 1992. Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. J Clin Microbiol 30 : 545–551.
  24. Aminoff D, 1961. Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids. Biochem J 81 : 384–392.
  25. Levine RL, Williams JA, Stadtman BR, Shacter E, 1994. Carbonyl assays for determination of oxidatively modified protein. Enzymology 233 : 346–357.
  26. Sedlak J, Lindsay RH, 1968. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem 25 : 192–205.
  27. Lindberg G, Eklund GA, Gullberg B, Rastam L, 1991. Serum sialic acid concentration and cardiovascular mortality. BMJ 302 : 143–146.
  28. Taniuchi K, Chifu K, Hayashi N, Nakamachi Y, Yamaguchi N, Miyamoto Y, Doi K, Baba S, Uchida Y, Tsukada Y, Sugimori T, 1981. A new enzymatic method for the determination of sialic acid in serum and its application for a marker of acute phase reactants. Kobe J Med Sci 27 : 91–102.
  29. Pickup JC, Mattock MB, Chusney GD, Burt D, 1997. NIDDM as a disease of the innate immune system: association of acute phase reactants and interleukin-6 with metabolic syndrome X. Diabetologia 40 : 1286–1292.
  30. Kushner I, 1993. Regulation of the acute phase response by cytokines. Perspect Biol Med 36 : 611–622.
  31. Baumann H, Gauldie J, 1994. The acute phase response. Immunol Today 15 : 74–80.
  32. Juffrie M, Meer GM, Hack CE, Haasnoot K, Sutaryo X, Veerman AJ, Thijs LG, 2001. Inflammatory mediators in dengue virus infection in children: interleukin-6 and its relation to C-reactive protein and secretory phospholipase A2. Am J Trop Med Hyg 65 : 70–75.
  33. Halliwell B, Gutteridge JM, 1990. Role of free radicals and catalytic metal ions in human disease: an overview. Methods Enzymol 186 : 1–85.
  34. Klassen P, Hans KB, Mazariegos M, Noel WS, Furst P, 2004. Classic dengue fever affects levels of circulating antioxidants. Nutrition 20 : 542–547.
  35. Goswami K, Koner BC, 2002. Level of sialic acid residues in Platelet proteins in diabetes, aging, and Hodgkin’s lymphoma: a potential role of free radicals in desialylation. Biochem Biophys Res Commun 297 : 502–505.
  36. Tanaka K, Tokumaru S, Kojo S, 1997. Possible involvement of radical reactions in desialylation of LDL. FEBS Lett 413 : 202–204.
  37. Bratosin D, Mazurier J, Tissier JP, Estaquier J, Huart JJ, Ameisen JC, Aminoff D, Montreuil J, 1998. Cellular and molecular mechanisms of senescent erythrocyte phagocytosis by macrophages. A review. Biochemie 80 : 173–195.
  38. Rothman AL, Ennis FA, 2000. Toga/flaviviruses: immunopathology. Cunningham MW, Fujinami RS, eds. Effects of Microbes on the Immune System. Philadelphia: Lippincott Williams and Wilkins, 473–490.
  39. Anderson R, Wang S, Osiowy C, Issekutz AC, 1997. Activation of endothelial cells via antibody-enhanced dengue virus infection of peripheral blood monocytes. J Virol 71 : 4226–4232.
  40. Granger DN, Rutili G, McCord J, 1981. Superoxide radicals in feline intestinal ischemia. Gastroenterology 81 : 22–29.
  41. Irani K, 2000. Oxidant signaling in vascular cell growth, death, and survival. Circ Res 87 : 179–183.
  42. Osu K, Inoves U, 2003. Correlation of increased platelet associated IgG and thrombocytopenia in dengue fever. J Med Virol 71 : 259–264.
  43. Schexneider KJ, Reedy EA, 2005. Thrombocytopenia in dengue fever. Current Hematol Resp 4 : 145–148.
  44. Erela O, Vurala H, Aksoya N, Aslanb G, Ulukan M, 2001. Oxidative stress of platelets and thrombocytopenia in patients with vivax malaria. Clin Biochem 34 : 341–344.

Data & Media loading...

  • Received : 29 Dec 2007
  • Accepted : 13 May 2008

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error