1921
Volume 79, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Glycerol-3-phosphate dehydrogenase (GPDH) isozymes are differentially expressed among tissues and during flight development. GPDH-1 is involved in the flight-muscle metabolism and GPDH-2 provides precursors for lipid biosynthesis in many tissues. We have isolated and characterized from , a Chagas disease vector, two cDNAs encoding for GPDH-1 and GPDH-2 isozymes. The inferred amino acid sequences showed high identity with other GPDH sequences from flying insects. A GPDH-2 transcript was found in fifth instar nymphs, thoracic muscles, adult gonads, and fat bodies. Both isozymes are present in 30-day-old adult thoracic muscle transcripts, and the pattern of expression differs between sexes. The expression of GPDH-1 begins earlier in females, and GPDH-2 is expressed more abundantly in female adult thoracic muscles than in those from males. This finding is consistent with those of other investigators who showed a higher flight initiation probability in females than in males.

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2008-07-01
2017-11-21
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References

  1. World Health Organization, 2002. Control of Chagas’ disease: second report of the WHO Expert Committee. World Health Organ Tech Rep Ser 905 : 1–109.
  2. Schmunis GA, 1999. Iniciativa del Cono Sur. Santo Domingo de Los Colorados, Ecuador, INDRE, Mexico City. Proceedings of the Second International Workshop on Population Biology and Control of Triatominae : 26–31
  3. Carcavallo RU, Jurberg J, Galindez Giron I, Lent H, 1997. Atlas of Chagas’ Disease Vector in the Americas. Rio de Janeiro: Editora Fiocruz.
  4. Schofield CJ, 1992. Dispersative flight by Triatoma infestans under natural climatic conditions in Argentina. Med Vet Entomol 6 : 51–56.
  5. Schofield CJ, Matthews JNS, 1985. Theoretical approach to active dispersal and colonization of houses by Triatoma infestans. J Trop Med Hyg 88 : 211–222.
  6. Cecere MC, Gürtler RE, Canale DM, Chuit R, Cohen JE, 2004. Effects of partial housing improvement and insecticide spraying on the reinfestation dynamics of Triatoma infestans in rural northwestern Argentina. Acta Trop 84 : 101–116.
  7. Vazquez-Prokopec GM, Ceballos LA, Kitron U, Gürtler GE, 2004. Active dispersal of natural populations of Triatoma infestans (Hemiptera: Reduviidae) in rural northwestern Argentina. J Med Entomol 41 : 614–621.
  8. Ceballos LA, Vazquez-Prokopec GM, Cecere MC, Gürtler RE, 2005. Seasonal variations and density-dependence of nutricional state and feeding rate of Triatoma infestans (Heteroptera: Reduviidae) in peridomestic ecotopes from northwestern Argentina. Acta Trop 95 : 149–159.
  9. Bewley GC, Cook JL, 1990. Molecular structure, developmental regulation, and evolution of the gene encoding glycerol-3-phosphate dehydrogenase isozymes in Drosophila melanogaster. Isozyme 3 : 341–374.
  10. Bewley GC, Rawls JM, Lucchesi JC, 1974. α-glycerolphosphate-dehydrogenase in Drosophila melanogaster: Kinetic differences and developmental differentiation of the larval and adult isozyme. J Insect Physiol 20 : 153–165.
  11. Collier GE, Sullivan DT, MacIntyre RJ, 1976. Purification of α-glycerophosphate dehydrogenase from Drosophila melanogaster. Biochim Biophys Acta 429 : 316.
  12. ÓBrien SJ and MacIntyre RJ, 1972. The alpha-glycerophosphate cycle in Drosophila melanogaster. Genet Aspects Genet. 71 : 127–138.
  13. Cook JL, Bewley GC, Shaffer JB, 1988. Drosophila α-glycerol-3-phosphate dehydrogenase isozymes are generated by alternate pathways of RNA processing resulting in different carboxyl-terminal amino acid sequence. J Biol Chem 263 : 10858–10864.
  14. Rechsteiner MD, 1970. Drosophila lactate dehydrogenase and α-glycerophosphate dehydrogenase: distribution and change in activity during development. J Insect Physiol 16 : 1179–1192.
  15. Wright TRF, Shaw CR, 1969. Genetics and ontogeny of α-glycerophosphate dehydrogenase isozymes in Drosophila melanogaster. Biochem Genet 3 : 343–353.
  16. Sacktor B, Dick A, 1962. Pathways of hydrogen transport of extra-mitochondrial reduced dephosphopyridine nucleotide in flight muscles. J Biol Chem 237 : 3259–3262.
  17. Scaraffia P, Remedi S, Maldonado C, Aoki A and Gerez de Burgos NM, 1997. Comparative enzymatic and ultrastructural changes in thoracic muscles of Triatomine insects during the last stage of metamorphosis. Biochem Physiol 116 : 173–179.
  18. Espinola NH, 1966. Note on sex differences in immature forms of Triatominae (Hemipter, Reduviidae). Rev Bras 26 : 263–267.
  19. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ, 1997. Basic local alignment search tool. J Mol Biol 215 : 403–410.
  20. Altschul SF, Lipman DJ, 1990. Protein database searches for multiple alignments. Proc Natl Acad Sci USA 87 : 5509–5513.
  21. Bairoch A, Apweiler R, 2000. The SWISS-PROT protein sequence database and its supplement TrEMBL in 2000. Nucleic Acids Res 28 : 45–48.
  22. Guex N, Peitsch MC, 1997. SWISS-MODEL and the Swiss-PdbViewer: An environment for comparative protein modeling. Electrophoresis 18 : 2714–2723.
  23. Schwede T, Kopp J, Guex N, Peitsch MC, 2003. SWISS-MODEL: an automated protein homology-modeling server. Nucleic Acid Res 31 : 3381–3385.
  24. Rasband WS, 1997–2007. ImageJ. Bethesda, MD: National Institutes of Health. Available at: http://rsb.info.nih.gov/ij/.
  25. Ou X, Ji C, Han X, Zhao X, Li X, Mao Y, Wong LL, Bartlam M, Rao Z, 2006. Crystal structures of human glycerol-3-phosphate dehydrogenase 1 (GPD1). J Mol Biol 357 : 858–869.
  26. Bewley GC, Cook JL, Kusakabe S, Makai T, Rigby DL Chambers GK, 1989. Sequence, structure and evolution of the gene coding for sn-glycerol-3-phosphate dehydrogenase in Drosophila melanogaster. Nucleic Acids Res 17 : 8553–8567.
  27. Wilanowski TM, Gibson, 1998. Sn-glycerol-3-phosphate dehydrogenase in the honey bee Apis mellifera: an unusual phenotype with the loss of introns. Gene 209 : 71–76.
  28. Popov VO, Lamzin VS, 1994. NAD+-dependent formate dehydrogenase. Biochem J 301 : 625–664.
  29. Gurevitz JM, Ceballos LA, 2006. Flight initiation of Triatoma infestans (Hemiptera: Reduviidae) under natural climatic conditions. J Med Entomol 43 : 143–150.
  30. Cavagnari BM, Scaraffia PY, Faller J, Gerez de Burgos NM, Santomé JA, 2000. Presence of fatty acid-biding and lipid stores in flight muscles of Dipetalogaster maximus (Hemíptera: Reduviidae). J Med Entomol 37 : 938–944.
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  • Received : 23 Nov 2007
  • Accepted : 01 Apr 2008

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