1921
Volume 79, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

The aim of this study was to evaluate the diffuse intralobular fibrosis in dogs naturally infected with () . One hundred five infected animals with positive serologic tests for were divided into two clinical groups: 69 symptomatic animals and 36 asymptomatic. Special staining with Gomori, Heidenhain, Silver, and Picrosirius Red was applied to characterize fibrilopoesis. The tissue parasite load was measured by immunohistochemistry and associated histomorphometric analyses. Intralobular fibrosis was observed in all dogs, and more collagen deposition was confirmed in the infected animals than in the controls by these histomorphometric studies. There were significant differences among the distinct clinical groups. In fact, symptomatic dogs showed an increased collagen deposition in the liver compared with asymptomatic ones. A peculiar diffuse intralobular fibrosis, where the collagen fibers encircled small groups of hepatocyte(s), was observed in two cases (1.9%).

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2008-08-01
2017-11-19
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References

  1. Deane LDD MP, 1955. Sobre a biologia do Phlebotomus longipalpis, transmissor da leishmaniose visceral em zona endêmica do Estado do Ceará. I. Distribuição, predominância e variação estacional. Rev Brasil Biol 15 : 83–95.
  2. Mauricio IL, Stothard JR, Miles MA, 2000. The strange case of Leishmania chagasi. Parasitol Today 16 : 188–189.
  3. Anosa VO, Idowu AL, 1983. The clinico-haematological features and pathology of leishmaniasis in a dog in Nigeria. Zentralbl Veterinarmed B 30 : 600–608.
  4. Keenan CM, Hendricks LD, Lightner L, Johnson AJ, 1984. Visceral leishmaniasis in the German shepherd dog. II. Pathology. Vet Pathol 21 : 80–86.
  5. Tafuri WL, Barbosa AJ, Michalick MS, Genaro O, Franca-Silva JC, Mayrink W, Nascimento E, 1996. Histopathology and immunocytochemical study of type 3 and type 4 complement receptors in the liver and spleen of dogs naturally and experimentally infected with Leishmania (Leishmania) chagasi. Rev Inst Med Trop Sao Paulo 38 : 81–89.
  6. Tafuri WL, de Oliveira MR, Melo MN, 2001. Canine visceral leishmaniosis: a remarkable histopathological picture of one case reported from Brazil. Vet Parasitol 96 : 203–212.
  7. Tryphonas L, Zawidzka Z, Bernard MA, Janzen EA, 1977. Visceral leishmaniasis in a dog: clinical, hematological and pathological observations. Can J Comp Med 41 : 1–12.
  8. Ferrer L, Rabanal RM, Domingo M, Ramos JA, Fondevila D, 1988. Identification of Leishmania donovani amastigotes in canine tissues by immunoperoxidase staining. Res Vet Sci 44 : 194–196.
  9. Solano-Gallego L, Fernandez-Bellon H, Morell P, Fondevila D, Alberola J, Ramis A, Ferrer L, 2004. Histological and immunohistochemical study of clinically normal skin of Leishmania infantuminfected dogs. J Comp Pathol 130 : 7–12.
  10. Lima WG, Michalick MS, de Melo MN, Luiz Tafuri W, 2004. Canine visceral leishmaniasis: a histopathological study of lymph nodes. Acta Trop 92 : 43–53.
  11. Martinez-Moreno A, Martinez-Cruz MS, Blanco A, Hernandez-Rodriguez S, 1993. Immunological and histological study of T-and B-lymphocyte activity in canine visceral leishmaniosis. Vet Parasitol 51 : 49–59.
  12. Ferrer L, Juanola B, Ramos JA, Ramis A, 1991. Chronic colitis due to Leishmania infection in two dogs. Vet Pathol 28 : 342–343.
  13. Silva FLT WL, Oliveira WMR, Tafuri WL, 2002. Histopathological and immunohistochemical study of the gastrointestinal tract from a dog naturally infected with Leishmania (Leishmania) chagasi. Arq. Bras. Med. Vet. Zoot. 54 : 340–344.
  14. Nieto CG, Navarrete I, Habela MA, Serrano F, Redondo E, 1992. Pathological changes in kidneys of dogs with natural Leishmania infection. Vet Parasitol 45 : 33–47.
  15. Tafuri WL, Michalick MS, Dias M, Genaro O, Leite VH, Barbosa AJ, Bambirra EA, da Costa CA, Melo MN, Mayrink W, 1989. [Optical and electron microscopic study of the kidney of dogs naturally and experimentally infected with Leishmania (Leishmania) chagasi]. Rev Inst Med Trop Sao Paulo 31 : 139–145.
  16. Oliveira GG, Santoro S, Sadigursky M, 1993. The subclinical form of experimental visceral leishmaniasis in dogs. Mem Inst Oswaldo Cruz 88 : 243–248.
  17. Andrade ZA, Andrade SG, 1966. [Some new aspects of the kala-azar pathology. (Morphologic study of 13 autopsy cases)]. Rev Inst Med Trop Sao Paulo 8 : 259–266.
  18. Bogliolo L, 1956. Nova contribuição ao conhecimento da anatomia patológica da leishmaniose visceral. A propósito de um caso brasileiro e com especial referência à fibrose hepática leishmaniótica. O Hospital 3 : 101–164.
  19. Chakravarty NK, Das Gupta B, Ray HN, Sen Gupta PC, 1956. The liver in kala-azar. Ann Trop Med Parasitol 50 : 252–259.
  20. Corbett CE, Duarte MI, Bustamante SE, 1993. Regression of diffuse intralobular liver fibrosis associated with visceral leishmaniasis. Am J Trop Med Hyg 49 : 616–624.
  21. Goswami BM, 1970. A study of cirrhosis of the liver due to chronic kala-azar. J Indian Med Assoc 54 : 315–320.
  22. Prata A, 1957. Estudo Clínico e Laboratorial do Calazar. Salvador: Universidade Federal da Bahia.
  23. Rogers L, 1908. A peculiar intralobular cirrhosisof the liver produced by the protozoal parasite of Kala-Azar. Ann Trop Med Parasitol 2 : 147–152.
  24. Guyot C, Lepreux S, Combe C, Doudnikoff E, Bioulac-Sage P, Balabaud C, Desmouliere A, 2006. Hepatic fibrosis and cirrhosis: the (myo)fibroblastic cell subpopulations involved. Int J Biochem Cell Biol 38 : 135–151.
  25. Seitz HM, 1995. [Parasitic diseases of the liver]. Verh Dtsch Ges Pathol 79 : 241–248.
  26. Tangkjvanich P, Yee HF Jr, 2002. Cirrhosiscan we reverse hepatic fibrosis? Eur J Surg 168 : 100–112.
  27. Aggarwal P, Wali JP, Chopra P, 1990. Liver in kala-azar. Indian J Gastroenterol 9 : 135–136.
  28. el Hag IA, Hashim FA, el Toum IA, Homeida M, el Kalifa M, el Hassan AM, 1994. Liver morphology and function in visceral leishmaniasis (Kala-azar). J Clin Pathol 47 : 547–551.
  29. Nattan-Larrier L, 1918. Les cirrhoses hepatiques due au Kalazar. Bull de l’Acad de Medicine 89 : 402–408.
  30. Wu CS, Chu IT, Wu TT, 1949. A case of kala-azar with cirrhosis of the liver and jaundice. J Pathol Bacteriol 61 : 209–215.
  31. Duarte MI, Corbett CE, 1984. Histopathological and ultrastructural aspects of interstitial pneumonitis of experimental visceral leishmaniasis. Trans R Soc Trop Med Hyg 78 : 683–688.
  32. Goncalves R, Tafuri WL, Melo MN, Raso P, 2003. Chronic interstitial pneumonitis in dogs naturally infected with Leishmania (Leishmania) chagasi: a histopathological and morphometric study. Rev Inst Med Trop Sao Paulo 45 : 153–158.
  33. Burns JM Jr, Shreffler WG, Benson DR, Ghalib HW, Badaro R, Reed SG, 1993. Molecular characterization of a kinesin-related antigen of Leishmania chagasi that detects specific antibody in African and American visceral leishmaniasis. Proc Natl Acad Sci USA 90 : 775–779.
  34. Houghton RL, Petrescu M, Benson DR, Skeiky YA, Scalone A, Badaro R, Reed SG, Gradoni L, 1998. A cloned antigen (recombinant K39) of Leishmania chagasi diagnostic for visceral leishmaniasis in human immunodeficiency virus type 1 patients and a prognostic indicator for monitoring patients undergoing drug therapy. J Infect Dis 177 : 1339–1344.
  35. Sundar S, Pai K, Sahu M, Kumar V, Murray HW, 2002. Immunochromatographic strip-test detection of anti-K39 antibody in Indian visceral leishmaniasis. Ann Trop Med Parasitol 96 : 19–23.
  36. De Paola DA, 1956. A Punção-Biópsia do Fígado. Valor da Pesquisa Histoquímica. Rio de Janeiro, Bol. Centro de Estudos HSE 8: 13.
  37. Junqueira LC, Bignolas G, Brentani RR, 1979. Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections. Histochem J 11 : 447–455.
  38. Caliari MV, 1997. Princípios de Morfometria Digital: KS300 Para Iniciantes. Belo Horizonte: Editora UFMG.
  39. Tafuri WL, Santos Rde L, Arantes RM, Goncalves R, de Melo MN, Michalick MS, 2004. An alternative immunohistochemical method for detecting Leishmania amastigotes in paraffin-embedded canine tissues. J Immunol Methods 292 : 17–23.
  40. Visentini A, 1910. Sull Anatomia Patologica del Kala-azar Osservato in Calabria e Sicilia. Studi Introno ad Alcune Malattie Tropicali della Calabria e della Sicillia. Roma: Tipografia Labicana. Part 2: 41–50.
  41. Laveran A, 1917. Leishmaniosis. In Masson e Cie (ed.), Paris.
  42. Duarte MI, Corbett CE, 1987. Histopathological patterns of the liver involvement in visceral leishmaniasis. Rev Inst Med Trop Sao Paulo 29 : 131–136.
  43. Da Silva JR, De Paola D, 1958. O problema da fibroses hepáticas na leishmaniose visceral americana. Gaz Med Port 11 : 361–378.
  44. Raso P, Siqueira JT, 1964. Subsídio ao conhecimento da anatomia patológica da leishmaniose visceral, com especial referência às lesões pulmonares e cardíacas. O Hospital 65 : 1291–1309.
  45. Anthony PP, Ishak KG, Nayak NC, Poulsen HE, Scheuer PJ, Sobin LH, 1978. The morphology of cirrhosis. Recommendations on definition, nomenclature, and classification by a working group sponsored by the World Health Organization. J Clin Pathol 31 : 395–414.
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  • Received : 23 Nov 2007
  • Accepted : 05 May 2008

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