1921
Volume 79, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

In an area with unstable malaria transmission, detection of infection in 379 symptomatic individuals was assessed by microscopy and three polymerase chain reaction (PCR) methodologies. infection was detected in 25% of patients by microscopy, 37% by nested PCR, 41% by merozoite surface protein-2 (MSP-2) PCR, and 45% by a ligase detection reaction-fluorescent microsphere assay (LDR-FMA). Of the 64 individuals who were LDR-FMA positive, microscopy negative and did not receive treatment, 8 (12.5%) had persistent symptoms and returned for treatment. Malaria attributable fraction (MAF) in symptomatic individuals was 14.6% by microscopy (95% confidence interval [CI] = 6.6–21.8%) and 28.2% by nested PCR (95% CI = 17.9–37.2%). In this highland area, infection in symptomatic individuals is detected more frequently by PCR than microscopy, and most frequently by LDR-FMA. infection appears to resolve without treatment in most LDR-FMA-positive, microscopy-negative individuals, but is persistent in a subset of these individuals and requires treatment.

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2008-08-01
2017-11-21
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  • Received : 13 Dec 2007
  • Accepted : 10 May 2008

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