1921
Volume 79, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Stool samples from 104 adult patients with nosocomial antibiotic–associated diarrhea were analyzed for by cultivation, toxin A inmunoenzymatic detection, and toxin B cytotoxic detection. The isolates were additionally screened for the toxin genes by polymerase chain reaction. was isolated from 26 samples, and the toxins were directly detected in another 5 samples. Toxin A and B genes were detected in all toxigenic bacterial isolates. The detection rate of 30% indicates that is a major etiologic agent of nosocomial diarrhea in Costa Rica.

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2008-08-01
2017-09-22
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References

  1. Asha NJ, Wilcox MH, 2002. Laboratory diagnosis of Clostridium perfringens antibiotic-associated diarrhoea. J Med Microbiol 51: 891–894.
  2. Legaria MC, Lumelsky G, Rosetti S, 2003. Clostridium difficile-associated diarrhea from a general hospital in Argentina. Anaerobe 9: 113–116.
  3. Noren T, Akerlund T, Back E, Sjoberg L, Persson I, Alriksson I, Burman LG, 2004. Molecular epidemiology of hospital-associated and community-acquired Clostridium difficile infection in a Swedish county. J Clin Microbiol 42: 3635–3643.
  4. Zheng L, Keller SF, Lyerly DM, Carman RJ, Genheimer CW, Gleaves CA, Kohlhepp SJ, Young S, Perez S, Ye K, 2004. Multicenter evaluation of a new screening test that detects Clostridium difficile in fecal specimens. J Clin Microbiol 42: 3837–3840.
  5. Alfa MJ, Du T, Beda G, 1998. Survey of incidence of Clostridium difficile infection in Canadian hospitals and diagnostic approaches. J Clin Microbiol 36: 2076–2080.
  6. O’Connor D, Hynes P, Cormican M, Collins E, Corbett-Feeney G, Cassidy M, 2001. Evaluation of methods for detection of toxins in specimens of feces submitted for diagnosis of Clostridium difficile-associated diarrhea. J Clin Microbiol 39: 2846–2849.
  7. Pituch H, Kreft D, Obuch-Woszczatynski P, Wultanska D, Meisel-Mikolajczyk F, Luczak M, van Belkum A, 2005. Clonal spread of a Clostridium difficile strain with a complete set of toxin A, toxin B, and binary toxin genes among Polish patients with Clostridium difficile-associated diarrhea. J Clin Microbiol 43: 472–475.
  8. Ferreira CE, Nakano V, Avila-Campos MJ, 2004. Cytotoxicity and antimicrobial susceptibility of Clostridium difficile isolated from hospitalized children with acute diarrhea. Anaerobe 10: 171–177.
  9. Brazier JS, Borriello SP, 2000. Microbiology, epidemiology and diagnosis of Clostridium difficile infection. Curr Top Microbiol Immunol 250: 1–33.
  10. Duerden B, Borriello SP, 1994. Report of the PHLS Clostridium difficile working group. PHLS Microbiology Diagnosis 11: 22–24.
  11. Thelestam M, Chaves-Olarte E, 2000. Cytotoxic effects of the Clostridium difficile toxins. Curr Top Microbiol Immunol 250: 85–96.
  12. van Dijck P, Avesani V, Delmee M, 1996. Genotyping of outbreak-related and sporadic isolates of Clostridium difficile belonging to serogroup C. J Clin Microbiol 34: 3049–3055.
  13. Barbut F, Lalande V, Burghoffer B, Thien HV, Grimprel E, Petit JC, 2002. Prevalence and genetic characterization of toxin A variant strains of Clostridium difficile among adults and children with diarrhea in France. J Clin Microbiol 40: 2079–2083.
  14. Wilkins TD, Lyerly DM, 2003. Clostridium difficile testing: after 20 years, still challenging. J Clin Microbiol 41: 531–534.
  15. Belanger SD, Boissinot M, Clairoux N, Picard FJ, Bergeron MG, 2003. Rapid detection of Clostridium difficile in feces by real-time PCR. J Clin Microbiol 41: 730–734.
  16. Turgeon DK, Novicki TJ, Quick J, Carlson L, Miller P, Ulness B, Cent A, Ashley R, Larson A, Coyle M, Limaye AP, Cookson BT, Fritsche TR, 2003. Six rapid tests for direct detection of Clostridium difficile and its toxins in fecal samples compared with the fibroblast cytotoxicity assay. J Clin Microbiol 41: 667–670.
  17. Pituch H, Obuch-Woszczatynski P, Luczak M, Meisel-Mikolajczyk F, 2003. Clostridium difficile and enterotoxigenic Bacteroides fragilis strains isolated from patients with antibiotic associated diarrhoea. Anaerobe 9: 161–163.
  18. Smedley JG 3rd, Fisher DJ, Sayeed S, Chakrabarti G, McClane BA, 2004. The enteric toxins of Clostridium perfringens. Rev Physiol Biochem Pharmacol 152: 183–204.
  19. Zhang G, Svenungsson B, Karnell A, Weintraub A, 1999. Prevalence of enterotoxigenic Bacteroides fragilis in adult patients with diarrhea and healthy controls. Clin Infect Dis 29: 590–594.
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  • Received : 28 Dec 2007
  • Accepted : 14 May 2008

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