Volume 79, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Stool samples from 104 adult patients with nosocomial antibiotic–associated diarrhea were analyzed for by cultivation, toxin A inmunoenzymatic detection, and toxin B cytotoxic detection. The isolates were additionally screened for the toxin genes by polymerase chain reaction. was isolated from 26 samples, and the toxins were directly detected in another 5 samples. Toxin A and B genes were detected in all toxigenic bacterial isolates. The detection rate of 30% indicates that is a major etiologic agent of nosocomial diarrhea in Costa Rica.


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  • Received : 28 Dec 2007
  • Accepted : 14 May 2008

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