1921
Volume 78, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

A total of 835 rodents captured in Beijing, China, were tested for hantavirus infection. Fifty-five (6.6%) were positive for viral RNA when lung tissue samples were examined by reverse transcriptase-polymerase chain reaction. Of 666 sera collected from the above rodents, 50 (7.5%) were positive for IgG antibody by ELISA. Among the 50 seropositive rodents, 37 were positive for viral RNA. In addition, five rodents were positive for viral RNA but negative for IgG antibody. The infection rates among study sites (χ = 28.93, df = 8, = 0.001) and habitats (χ = 22.88,df = 7, = 0.02) were significantly different. The sequences of partial M-segment of hantaviruses detected in 11 representative rodents had 0.1–8.2% divergence. Phylogenetic analysis showed that our hantavirus sequences fell into three different lineages regardless of geographical origin or rodent species. A strain detected from a trading center of agricultural products, which might be imported from other provinces, was genetically different from other strains of Beijing.

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2017-11-18
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References

  1. Plyusnin A, Vapalahti O, Vaheri A, 1996. Hantaviruses: genome structure, expression and evolution. J Gen Virol 77 : 2677–2687.
  2. International Committee on taxonomy of viruses. ICTVdB—The Universal Virus Database, version 4. Available at: http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/index.htm. Accessed 2006.
  3. Joe HS, Lela KR, 2002. Hantaviruses: an overview. Comp Med 52 : 97–110.
  4. Schmaljohn CS, Hjelle B, 1997. Hantaviruses: a global disease problem. Emerg Infect Dis 3 : 95–104.
  5. Liu G, Li C, Hu GW, Li Y, Yao LS, Chen YQ, Ren M, Guan SX, Yu CY, Na BZ, Zhong XD, Sun YX, Li XW, Li DX, 2003. Identification of Puumala like viruses in China. Chin Exp Clin Virol 17 : 55–57.
  6. Song G, 1999. Epidemiological progresses of hemorrhagic fever with renal syndrome in China. Chin Med J (Engl) 112 : 472–477.
  7. Bai XF, Huang CS, 2002. Study farther on hemorrhagic fever with renal syndrome. Chin J Infect Dis 20 : 197–198.
  8. Zhang YZ, Xiao DL, Wang Y, 2004. The epidemic characteristics and preventive measure of hemorrhagic fever with syndromes in China. Chin J Epidemiol 25 : 466–469.
  9. Zhang XC, Hu JY, Zhou S, 2001. Study on HFRS epidemic state in Beijing. Chin J Vector Biol Control 12 : 218–221.
  10. Zhang YH, Chen HX, 1997. Typing of hantavirus from lung tissues of R. norvegicus by RT-PCR and sequences analysis. Chin J Vector Biol Control 8 : 361–367.
  11. Wang H, Yoshimatsu K, Ebihara H, Ogino M, Araki K, Kariwa H, Wang ZX, Luo ZZ, Li DX, Hang CS, Arikawa J, 2000. Genetic diversity of hantaviruses isolated in China and characterization of novel hantaviruses isolated from Niviventer confucianus and Rattus rattus. Virology 278 : 332–345.
  12. Kim EC, Kim IS, Choi Y, Kim SG, Lee J, 1994. Rapid differentiation between Hantaan and Seoul viruses by polymerase chain reaction and restriction enzyme analysis. J Med Virol 43 : 245–248.
  13. Pan L, Bai XF, 2001. Typing of hantavirus from patient RT-PCR and sequences analysis. Chin J Vector Biol Control 12 : 234–237.
  14. Burland TG, 2000. DNASTAR Lasergene sequence analysis software. Methods Mol Biol 132 : 71–91.
  15. Retief JD, 2000. Phylogenetic analysis using PHYLIP. Methods Mol Biol 132 : 243–258.
  16. Felsenstein J, 2004. Phylip3.63 software package. Available at: http://evolution.genetics.washington.edu/phylip.html. Accessed 2004.
  17. Hu JS, 1993. Survey on HFRS in Beijing. Chin J Vector Biol Control 4 : 34–37.
  18. Zuo SQ, Zhang PH, Wu XM, Zhan L, Jiang JF, Cao WC, 2005. Isolation and identification of hantavirus from Rattus norvegicus in Beijing. Chin J Microbiol Immunol 25 : 367–368.
  19. Jiang JF, Wu XM, Zuo SQ, Wang RM, Chen LQ, Wang BC, Dun Z, Zhang PH, Guo TY, Cao WC, 2006. Study on the association between hantavirus infection and Rattus norvegicus. Chin J Epidemiol 3 : 196–199.
  20. Plyusnina A, Ibrahim IN, Winoto I, Porter KR, Gotama IB, Lundkvist A, Vaheri A, Plyusnin A, 2004. Identification of Seoul hantavirus in Rattus norvegicus in Indonesia. Scand J Infect Dis 36 : 356–359.
  21. Heyman P, Plyusnina A, Berny P, Cochez C, Artois M, Zizi M, Pirnay JP, Plyusnin A, 2004. Seoul hantavirus in Europe: first demonstration of the virus genome in wild Rattus norvegicus captured in France. Eur J Clin Microbiol Infect Dis 23 : 711–717.
  22. Diglisic G, Xiao SY, Gligic A, Obradovic M, Stojanovic R, Velimirovic D, Lukac V, Rossi CA, LeDuc JW, 1994. Isolation of a Puumala-like virus from Mus musculus captured in Yugoslavia and its association with severe hemorrhagic fever with renal syndrome. J Infect Dis 169 : 204–207.
  23. Weidmann M, Schmidt P, Vackova M, Krivanec K, Munclinger K, Hufert FT, 2005. Identification of genetic evidence for Dobrava virus spillover in rodents by nested reverse transcription (RT)-PCR and TaqMan RT-PCR. J Clin Microbiol 43 : 808–812.
  24. Vapalahti O, Mustonen J, Lundkvist Å, Henttonen H, Plyusnin A, Vaheri A, 2003. Hantavirus infections in Europe. Lancet Infect Dis 3 : 653–661.
  25. Jiang JF, Wu XM, Zuo SQ, Wang RM, Chen LQ, Wang BC, Dun Z, Zhang PH, Guo TY, Cao WC, 2005. Study on association between variation of hantavirus and DNA genomic RAPD marker of different Rattus norvegicus. Chin J Vector Biol Control 16 : 358–363.
  26. Feuer R, Boone JD, Netski D, Morzunov SP, St Jeor SC, 1999. Temporal and spatial analysis of Sin Nombre virus quasispecies in naturally infected rodents. J Virol 73 : 9544–9554.
  27. Isegawa Y, Tanishita O, Ueda S, Yamanishi K, 1994. Association of serine in position 1124 of Hantaan virus glycoprotein with virulence in mice. J Gen Virol 75 : 3273–3278.
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  • Received : 25 Jan 2006
  • Accepted : 10 Sep 2007

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