Volume 78, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Patent and pathologic infections of the human hookworm were established in the common marmoset (). In a pilot study, a laboratory strain of was compared with a fresh field isolate. Pathology was more severe in animals infected with a fresh isolate. In all animals, infection was associated with increased total plasma IgE and production of IgG specific to adult worm excretory/secretory (ES) products. Histamine was released by basophils in response to IgE, ES products, and a recombinant hookworm allergen, calreticulin. The pilot study indicated the potential of this animal model of hookworm infection and led us to investigate the consequences of infecting a further cohort with the fresh field isolate. This second study confirmed our initial findings, that it is possible to investigate the human hookworm in a model exhibiting many of the characteristics of the immunology of hookworm infection in its definitive host.


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  1. de Silva NR, Brooker S, Hotez PJ, Montressor A, Engels D, Savioli L, 2003. Soil transmitted helminth infections: updating the global picture. Trends Parasitol 19 : 547–551. [Google Scholar]
  2. Chan M, Medley G, Bundy D, 1994. The evaluation of potential global morbidity attributable to intestinal nematode infection. Parasitology 109 : 373–387. [Google Scholar]
  3. Hotez P, Pritchard DI, 1995. Hookworm infection. Sci Am 272 : 42–48. [Google Scholar]
  4. Stephenson L, 2001. Pathophysiology of intestinal nematodes. Holland C, Kennedy M, eds. World Class Parasites: The Geohelminths: Ascaris, Trichuris and Hookworm. Boston: Kluwer Academic Press, 39–61.
  5. Ghosh K, Hotez P, 1999. Antibody dependent reductions in mouse hookworm burden after vaccination with Ancylostoma caninum secreted protein 1. J Infect Dis 180 : 1674–1681. [Google Scholar]
  6. Hotez P, Ghosh K, Hawdon J, Narasimhan S, Jones B, Shuhua X, Sen L, Bin Z, Haechou X, Hainan R, Heng W, Koski RA, 1999. Experimental approaches to the development of a recombinant hookworm vaccine. Immunol Rev 171 : 163–172. [Google Scholar]
  7. Sen L, Ghosh K, Zhan B, Shan Q, Thompson M, Hawdon J, Koski RA, Xiao SH, Hotez PJ, 2000. Hookworm burden reductions in BALB/c mice vaccinated with Ancylostoma secreted protein 1 (ASP-1) from Ancylostoma duodenale, A caninum and Necator americanus. Vaccine 18 : 1096–1102. [Google Scholar]
  8. Culley F, Brown A, Girod N, Pritchard D, Williams T, 2000. Innate and cognate mechanisms of pulmonary eosinophillia in helminth infection. Eur J Immunol 32 : 1376–1385. [Google Scholar]
  9. Girod N, Brown A, Pritchard D, Billet E, 2003. Successful vaccination of BALB/c mice against human hookworm (N. americanus): the immunological phenotype of the protective response. Int J Parasitol 33 : 71–80. [Google Scholar]
  10. Sen H, Seth D, 1967. Complete development of the human hookworm, Necator americanus, in golden hamsters, Mesocricetus auratus. Nature 214 : 609–610. [Google Scholar]
  11. Behnke J, Hamid S, Pritchard D, Rajasekariah G, 1984. Necator americanus in laboratory rodents. Parasitology 89 : R40. [Google Scholar]
  12. Behnke J, Wells C, Brown J, 1986. An improved technique for experimental infections with skin penetrating nematode larvae (Necator americanus). Int J Parasitol 16 : 461–464. [Google Scholar]
  13. Behnke J, Paul V, Rajasekariah G, 1986. The growth and migration of Necator americanus following infection of neonatal hamsters. Trans R Soc Trop Med Hyg 80 : 146–149. [Google Scholar]
  14. Kumar S, Pritchard DI, 1992. The partial characterisation of proteases present in the excretory/secretory products and exsheathing fluid of the infective (L3) larva of Necator americanus. Int J Parasitol 22 : 563–572. [Google Scholar]
  15. Harada Y, Mori O, 1955. A new method of culturing hookworms. Yonago Acta Med 1: 177–179. [Google Scholar]
  16. Brown A, Pritchard DI, 1993. The immunogenicity of hookworm (Necator americanus) acetylcholinesterase (AChE) in man. Parasite Immunol 15 : 195–203. [Google Scholar]
  17. Beynon R, Bond J, 1993. Proteolytic Enzymes: A Practical Approach. Oxford, United Kingdom: IRL Press.
  18. Furmidge BA, Horn LA, Pritchard DI, 1995. The antihaemostatic strategies of the human hookworm Necator americanus. Parasitology 112 : 81–87. [Google Scholar]
  19. Kasper G, Brown A, Eberl M, Vallar L, Kieffer N, Berry C, Girdwood K, Eggleton P, Quinnell RJ, Pritchard DI, 2001. A calreticulin-like molecule from the human hookworm Necator americanus interacts with C1q and the cytoplasmic signalling domains of some integrins. Parasite Immunol 23 : 141–152. [Google Scholar]
  20. Morton D, Abbot D, Barclay R, Close B, Ewbank R, Gask D, Heath M, Mattic S, Poole T, Seamer J, Southee J, Thompson A, Trussell B, West C, Jennings M, 1993. Removal of blood from laboratory mammals and birds-first report of the BVA/ FRAME/RSPCA/UFAW joint working group on refinement. Lab Anim 27 : 1–22. [Google Scholar]
  21. Pritchard DI, Quinnell RJ, Slater AFG, McKean PG, Dale DDS, Raiko A, Keymer AE, 1990. Parasitological, haematological and immunological observations in a community infected with the hookworm Necator americanus in Papua New Guinea. Parasitology 100 : 317–326. [Google Scholar]
  22. Laemmli U, 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 : 680–685. [Google Scholar]
  23. Towbin H, Staehelin T, Gordon J, 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76 : 4350–4354. [Google Scholar]
  24. Griffiths GD, Hornby RJ, Jagger CP, Brown AP, Stoten A, Pearce PC, Scott L, Pritchard DI, 2006. Development of methods to measure humoral immune responses against selected antigens in the common marmoset (Callithrix jacchus) and the effect of pyridostigmine bromide administration. Int Immunopharmacol 6 : 1755–1764. [Google Scholar]
  25. Gill G, Frost J, Miller K, 1974. A new formula for a half-oxidized haematoxylin solution that neither overstains or requires differentiation. Acta Cytol 18 : 300–311. [Google Scholar]
  26. Phillips C, Coward WR, Pritchard DI, Hewitt CRA, 2003. Basophils express a type-2 cytokine profile on exposure to proteases from helminths and house dust mites. J Leuc Biol 73 : 165–171. [Google Scholar]
  27. Carr A, Pritchard DI, 1987. Antigen expression during development of the human hookworm, Necator americanus (Nematoda). Parasite Immunol 9 : 219–234. [Google Scholar]
  28. Prtichard D, Quinnell R, Walsh E, 1995. Immunity in humans to N. americanus: IgE, parasite weight and fecundity. Parasite Immunol 71 : 71–75. [Google Scholar]
  29. Pritchard DI, Brown A, Kasper G, McElroy P, Loukas A, Hewitt C, Berry C, Füllkrug R, Beck E, 1999. A hookworm allergen that strongly resembles calreticulin. Parasite Immunol 21 : 439–450. [Google Scholar]
  30. Ogilvie BM, Bartlett A, Godfrey RC, Turton JA, Worms MJ, Yeates RA, 1978. Antibody responses in self infections with Necator americanus. Trans R Soc Trop Med Hyg 72 : 66–71. [Google Scholar]
  31. Scrivener S, Yemaneberhan H, Zebenigus M, Tilahun D, Girma S, Ali S, McElroy P, Custovic A, Woodcock A, Pritchard DI, Venn A, Britton J, 2001. Independent effects of intestinal parasite infection and domestic allergen exposure on the risk of wheeze in Ethiopia. Lancet 358 : 1493–1499. [Google Scholar]
  32. Hotez P, 1989. Hookworm disease in children. Ped Infect Dis J 8 : 516–520. [Google Scholar]
  33. Stephenson LS, 2002. Pathophysiology of intestinal nematodes. Holland C, Kennedy M, eds. World Class Parasites: The Geohelminths: Ascaris, Trichuris and Hookworm. Boston: Kluwer Academic Press, 39–62.
  34. Hart B, vanMeurs M, Brok H, Massacesi L, Bauer J, Boon L, Bontrop RE, Laman JD, 2000. A new primate model for multiple sclerosis in the common marmoset. Immunol Today 21 : 290–297. [Google Scholar]

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  • Received : 01 Dec 2005
  • Accepted : 31 Jan 2008

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