1921
Volume 78, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Molecular technology has led to the discovery of previously unrecognized species in new hosts, such as in humans. The notion that dogs may transmit species to humans has significant public health implications, and additional studies are merited. The purpose of this study was to examine a group of kenneled dogs to determine the prevalence of species infection and to identify parasite species. Prevalence of active infection was 71%. Six positive samples were analyzed by polymerase chain reaction amplification of the 18S ribosomal RNA gene followed by restriction fragment length polymorphism analysis to identify the species. Restriction digest patterns identified as the infecting species in all six dogs; species identity was confirmed by genetic sequencing. To our knowledge, this is the first report of a naturally occurring infection in a canine host. The finding of in asymptomatic canines supports the notion of dogs as potential sources of human infection.

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2008-06-01
2017-11-20
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References

  1. Fayer R, 2004. Cryptosporidium: a water-borne zoonotic parasite. Vet Parasitol 126 : 37–56.
  2. Betancourt WQ, Rose JB, 2004. Drinking water treatment processes for removal of Cryptosporidium and Giardia. Vet Parasitol 126 : 219–234.
  3. Caccio SM, 2005. Molecular epidemiology of human cryptosporidiosis. Parassitologia 47 : 185–192.
  4. Iseki M, Maekawa T, Moriya K, Uni S, Takada S, 1989. Infectivity of Cryptosporidium muris (strain RN 66) in various laboratory animals. Parasitol Res 75 : 218–222.
  5. Aydin Y, Ozkul IA, 1996. Infectivity of Cryptosporidium muris directly isolated from the murine stomach for various laboratory animals. Vet Parasitol 66 : 257–262.
  6. Xiao L, Ryan UM, 2004. Cryptosporidiosis: an update in molecular epidemiology. Curr Opin Infect Dis 17 : 483–490.
  7. Miller DL, Liggett A, Radi ZA, Branch LO, 2003. Gastrointestinal cryptosporidiosis in a puppy. Vet Parasitol 115 : 199–204.
  8. Abe N, Sawano Y, Yamada K, Kimata I, Iseki M, 2002. Cryptosporidium infection in dogs in Osaka, Japan. Vet Parasitol 108 : 185–193.
  9. Irwin PJ, 2002. Companion animal parasitology: a clinical perspective. Int J Parasitol 32 : 581–593.
  10. el-Ahraf A, Tacal JV Jr, Sobih M, Amin M, Lawrence W, Wilcke BW, 1991. Prevalence of cryptosporidiosis in dogs and human beings in San Bernardino County, California. J Am Vet Med Assoc 198 : 631–634.
  11. Hackett T, Lappin MR, 2003. Prevalence of enteric pathogens in dogs of north-central Colorado. J Am Anim Hosp Assoc 39 : 52–56.
  12. Xiao L, Cama VA, Cabrera L, Ortega Y, Pearson J, Gilman RH, 2007. Possible transmission of Cryptosporidium canis between children and a dog in a household. J Clin Microbiol 45 : 2014–2016.
  13. Tzipori S, Campbell I, 1981. Prevalence of Cryptosporidium antibodies in 10 animal species. J Clin Microbiol 14 : 455–456.
  14. Garcia LS, Shimizu RY, 1997. Evaluation of nine immunoassay kits (enzyme immunoassay and direct fluorescence) for detection of Giardia lamblia and Cryptosporidium parvum in human fecal specimens. J Clin Microbiol 35 : 1526–1529.
  15. Rimhanen-Finne R, Enemark HL, Kolehmainen J, Toropainen P, Hanninen ML, 2007. Evaluation of immunofluorescence microscopy and enzyme-linked immunosorbent assay in detection of Cryptosporidium and Giardia infections in asymptomatic dogs. Vet Parasitol 145 : 345–348.
  16. Xiao L, Morgan UM, Limor J, Escalante A, Arrowood M, Shulaw W, Thompson RC, Fayer R, Lal AA, 1999. Genetic diversity within Cryptosporidium parvum and related Cryptosporidium species. Appl Environ Microbiol 65 : 3386–3391.
  17. Xiao L, Escalante L, Yang C, Sulaiman I, Escalante AA, Montali RJ, Fayer R, Lal AA, 1999. Phylogenetic analysis of Cryptosporidium parasites based on the small-subunit rRNA gene locus. Appl Environ Microbiol 65 : 1578–1583.
  18. Kumar S, Tamura K, Jakobsen IB, Nei M, 2001. MEGA2: molecular evolutionary genetics analysis software. Bioinformatics 17 : 1244–1245.
  19. Fayer R, Morgan U, Upton SJ, 2000. Epidemiology of Cryptosporidium: transmission, detection and identification. Int J Parasitol 30 : 1305–1322.
  20. Warren KS, Swan RA, Morgan-Ryan UM, Friend JA, Elliot A, 2003. Cryptosporidium muris infection in bilbies (Macrotis lagotis). Aust Vet J 81 : 739–741.
  21. Simpson JW, Burnie AG, Miles RS, Scott JL, Lindsay DI, 1988. Prevalence of Giardia and Cryptosporidium infection in dogs in Edinburgh. Vet Rec 123 : 445.
  22. Grimason AM, Smith HV, Parker JF, Jackson MH, Smith PG, Girdwood RW, 1993. Occurrence of Giardia sp. cysts and Cryptosporidium sp. oocysts in faeces from public parks in the west of Scotland. Epidemiol Infect 110 : 641–645.
  23. Causape AC, Quilez J, Sanchez-Acedo C, del Cacho E, 1996. Prevalence of intestinal parasites, including Cryptosporidium parvum, in dogs in Zaragoza city, Spain. Vet Parasitol 67 : 161–167.
  24. Kim JT, Wee SH, Lee CG, 1998. Detection of Cryptosporidium oocysts in canine fecal samples by immunofluorescence assay. Korean J Parasitol 36 : 147–149.
  25. Bugg RJ, Robertson ID, Elliot AD, Thompson RC, 1999. Gastrointestinal parasites of urban dogs in Perth, Western Australia. Vet J 157 : 295–301.
  26. Cirak VY, Bauer C, 2004. Comparison of conventional coproscopical methods and commercial coproantigen ELISA kits for the detection of Giardia and Cryptosporidium infections in dogs and cats. Berl Munch Tierarztl Wochenschr 117 : 410–413.
  27. Nakai Y, Hikosaka K, Sato M, Sasaki T, Kaneta Y, Okazaki N, 2004. Detection of Cryptosporidium muris type oocysts from beef cattle in a farm and from domestic and wild animals in and around the farm. J Vet Med Sci 66 : 983–984.
  28. Huber F, Bomfim TC, Gomes RS, 2005. Comparison between natural infection by Cryptosporidium sp., Giardia sp. in dogs in two living situations in the West Zone of the municipality of Rio de Janeiro. Vet Parasitol 130 : 69–72.
  29. Giangaspero A, Iorio R, Paoletti B, Traversa D, Capelli G, 2006. Molecular evidence for Cryptosporidium infection in dogs in central Italy. Parasitol Res 99 : 297–299.
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  • Received : 12 Oct 2007
  • Accepted : 11 Mar 2008

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