Volume 78, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


We studied the use of African water storage pots for point source application of against the malaria vectors and . Clay pots were shown to be attractive resting sites for male and female and were not repellent after impregnation with fungus. was highly infective and virulent after spray application inside pots. At a dosage of 4 × 10 conidia/m, an average of 95 ± 1.2% of obtained a fungal infection. A lower dosage of 1 × 10 conidia/m infected an average of 91.5 ± 0.6% of and 91.8 ± 1.2% of mosquitoes. Fungal infection significantly reduced mosquito longevity, as shown by differences between survival curves and LT values. These pots are suitable for application of entomopathogenic fungi against malaria vectors and their potential for sustainable field implementation is discussed.


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  1. WHO, 2005. The World Malaria Report 2005. Geneva, Switzerland: WHO.
  2. White GB, 1974. Anopheles gambiae complex and disease transmission in Africa. Trans R Soc Trop Med Hyg 68 : 278–302.
  3. Coetzee M, Craig M, Le Sueur D, 2000. Distribution of African malaria mosquitoes belonging to the Anopheles gambiae complex. Parasitol Today 16 : 74–77.
  4. Rogers DJ, Randolph SE, Snow RW, Hay SI, 2002. Satellite imagery in the study and forecast of malaria. Nature 415 : 710–715.
  5. Mbogo CM, Mwangangi JM, Nzovu J, Gu WD, Yan GY, Gunter JT, Swalm C, Keating J, Regens JL, Shililu JI, Githure JI, Beier JC, 2003. Spatial and temporal heterogeneity of Anopheles mosquitoes and Plasmodium falciparum transmission along the Kenyan coast. Am J Trop Med Hyg 68 : 734–742.
  6. Gillies MT, de Meillon B, 1968. The Anophelinae of Africa South of the Sahara. Johannesburg, South Africa: South African Institute for Medical Research.
  7. N’Guessan R, Corbel V, Akogbeto M, Rowland M, 2007. Reduced efficacy of insecticide-treated nets and indoor residual spraying for malaria control in pyrethroid resistance area. Benin Emerg Infect Dis 13 : 199–206.
  8. Roy HE, Steinkraus DC, Eilenberg J, Hajek AE, Pell JK, 2006. Bizarre interactions and endgames: Entomopathogenic fungi and their arthropod hosts. Annu Rev Entomol 51 : 331–357.
  9. Zimmermann G, 1993. The entomopathogenic fungus Metarhizium anisopliae and its potential as a biocontrol agent. Pestic Sci 37 : 375–379.
  10. Environmental Protection Agency, 2003. Metarhizium anisopliae strain F52 (029056) Biopesticide Fact Sheet. Available at: http://www.epa.gov/oppbppd1/biopesticides/ingredients/factsheets/factsheet_029056.htm. Accessed November 5, 2007.
  11. Bateman R, 2004. Constraints and enabling technologies for mycopesticide development. Outlooks Pest Manag 15 : 64–69.
  12. Feng MG, Poprawski TJ, Khachatourians GG, 1994. Production, formulation and application of the entomopathogenic fungus Beauveria bassiana for insect control: current status. Biocontrol Sci Technol 4 : 3–34.
  13. de Faria MR, Wraight SP, 2007. Mycoinsecticides and Mycoacaricides: a comprehensive list with worldwide coverage and international classification of formulation types. Biol Control 43 : 237–256.
  14. Douthwaite B, Langewald J, Harris J, 2001. IMPACT: Development and Commercialization of the Green Muscle Biopesticide. Ibadan, Nigeria: International Institute of Tropical Agriculture.
  15. Khetan SK, 2001. Mycoinsecticides. In: Microbial Pest Control. Khetan SK (Editor). Marcel Dekker, New York.
  16. Scholte EJ, Njiru BN, Smallegange RC, Takken W, Knols BGJ, 2003. Infection of malaria (Anopheles gambiae s.s.) and filariasis (Culex quinquefasciatus) vectors with the entomopathogenic fungus Metarhizium anisopliae. Malar J 2 : 29.
  17. Scholte E-J, Ng’habi K, Kihonda J, Takken W, Paaijmans K, Abdulla S, Killeen GF, Knols BGJ, 2005. An entomopathogenic fungus for control of adult African malaria mosquitoes. Science 308 : 1641–1642.
  18. Scholte EJ, Takken W, Knols BGJ, 2007. Infection of adult Aedes aegypti and Ae. albopictus mosquitoes with the entomopathogenic fungus Metarhizium anisopliae. Acta Trop 102 : 151–158.
  19. Blanford S, Chan BHK, Jenkins N, Sim D, Turner RJ, Read AF, Thomas MB, 2005. Fungal pathogen reduces potential for malaria transmission. Science 308 : 1638–1641.
  20. Scholte E-J, Knols BGJ, Takken W, 2006. Infection of the malaria mosquito Anopheles gambiae with the entomopathogenic fungus Metarhizium anisopliae reduces blood feeding and fecundity. J Invertebr Pathol 91 : 43–49.
  21. Thomas M, Read AF, 2007. Can fungal biopesticides control malaria? Nat Rev 5 : 377–383.
  22. Knols BGJ, Thomas MB, 2006. Fungal entomopathogens for adult mosquito control—A look at the prospects. Outlooks Pest Managt 17 : 257–259.
  23. Farenhorst M, Knols BGJ, 2007. Fungal entomopathogens for the control of adult mosquitoes: a look at the issues. Proc Neth Entomol Soc Meet 18 : 51–59.
  24. Odiere M, Bayoh MN, Gimnig J, Vulule J, Irungu L, Walker E, 2007. Sampling outdoor, resting Anopheles gambiae and other mosquitoes (Diptera: Culicidae) in Western Kenya with clay pots. J Med Entomol 44 : 14–22.
  25. Hunt RH, Brooke BD, Pillay C, Koekemoer LL, Coetzee M, 2005. Laboratory selection for and characteristics of pyrethroid resistance in the malaria vector Anopheles funestus. Med Vet Entomol 19 : 271–275.
  26. Scholte EJ, Knols BGJ, Takken W, 2004. Autodissemination of the entomopathogenic fungus Metarhizium anisopliae amongst adults of the malaria vector Anopheles gambiae s.s. Malar J 3 : 45.
  27. Scholte EJ, 2004. A study of avoidance and repellency of the african malaria vector An. gambiae s.s. upon exposure to the entomopathogenic fungus Metarhizium anisopliae. PhD thesis. Wageningen, The Netherlands: Wageningen University.
  28. Clements AN, 1992. The biology of mosquitoes. Vol. 1. Development, nutrition and reproduction. Chapman & Hall, London: 509 pp.
  29. Zwiebel LJ, Takken W, 2004. Olfactory regulation of mosquito–host interactions. Insect Biochem Mol Biol 34 : 645–652.
  30. Njiru BN, Mukabana WR, Takken W, Knols BGJ, 2006. Trapping of the malaria vector Anopheles gambiae with odour-baited MM-X traps in semi-field conditions in western Kenya. Malar J 5 : 39.
  31. Healy TP, Copland MJW, Cork A, Przyborowska A, Halket JM, 2002. Landing responses of Anopheles gambiae elicited by oxo-carboxylic acids. Med Vet Entomol 16 : 126–132.
  32. Roberts DR, Alecrim WD, Hshieh P, Grieco JP, Bangs M, Andre RG, Chareonviriphap T, 2000. A probability model of vector behavior: effects of DDT repellency, irritancy, and toxicity in malaria control. J Vector Ecol 25 : 48–61.

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  • Received : 17 Dec 2007
  • Accepted : 22 Feb 2008

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