Volume 78, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Cervical cancer is believed to have a co-factorial etiology in which high-risk human papillomavirus (HPV) infections are considered an essential factor and other elements play an ancillary role. Besides the importance of specific HPV genotypes, other viral cofactors as viral load may influence the progression likelihood. In this study the relationship between HPV 16 viral load with respect to the grade of cervical disease in Honduran women was investigated. A real-time PCR allowing quantification of both HPV 16 genome and β-globin gene to normalize the measuring HPV 16 load in cervical cells was used. The data in 87 women with cervical dysplasia or cervical cancer and in 23 women with a negative Pap smear were evaluated. The highest average of HPV 16 viral load was detected in women with High Squamous Intraepithelial Lesions (HSIL). An increasing amount of HPV in higher cervical lesions was found, which could indicate a dose-response association between viral load and precancerous lesion grade.


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  1. Arossi S, Sankaranarayanan R, Parkin DM, 2003. Incidence and mortality of cervical cancer in Latin America. Salud Publica Mex 45 : 306–314. [Google Scholar]
  2. Ferlay J, Bray F, Pisani P, Parkin DM, 2004. GLOBOCAN 2002: Cancer Incidence, Mortality and Prevalence Worldwide. Lyon: IARC Press, Version 2.0. IARC Cancer Base No. 5.
  3. Josefsson AM, Magnusson PK, Ylitalo N, Sorensen P, Qwar-forth-Tubbin P, Andersen PK, Melbye M, Adami HO, Gyllensten UB, 2000. Viral load of human papilloma virus 16 as a determinant for development of cervical carcinoma in situ: a nested case-control study. Lancet 355 : 2189–2193. [Google Scholar]
  4. Swan DC, Tucker RA, Tortolero-Luna G, Mitchell MF, Wideroff L, Unger ER, Nisenbaum RA, Reeves WC, Icenogle JP, 1999. Human papillomavirus (HPV) DNA copy number is dependent on grade of cervical disease and HPV type. J Clin Microbiol 37 : 1030–1034. [Google Scholar]
  5. van Duin M, Snijders PJ, Schrijnemakers HF, Voorhorst FJ, Rozendaal L, Nobbenhuis MA, van den Brule AJ, Verheijen RH, 2002. Human papillomavirus 16 load in normal and abnormal cervical scrapes: an indicator of CIN II/III and viral clearance. Int J Cancer 98 : 590–595. [Google Scholar]
  6. Ylitalo N, Sorensen P, Josefsson AM, Magnusson PK, Andersen PK, Ponten J, Adami HO, Gyllensten UB, Melbye M, 2000. Consistent high viral load of human papillomavirus 16 and risk of cervical carcinoma in situ: a nested case-control study. Lancet 355 : 2194–2198. [Google Scholar]
  7. Boom R, Sol CJA, Salimans MM, Jansen CL, van Wetheim PME, van der Noordaa J, 1990. Rapid and simple method for purification of nucleic acids. J Clin Microbiol 28 : 495–503. [Google Scholar]
  8. Kleter B, van Doorn LJ, Schegget J, Schrauwen L, van Krimpen C, Burgear M, Harmsel B, Quint W, 1998. Novel short fragment PCR assay for highly sensitive broad-spectrum detection of anogenital human papillomaviruses. Am J Pathol 153 : 1731–1739. [Google Scholar]
  9. Melchers WJ, Bakkers JM, Wang J, de Wilde PCM, Boonstra H, Quint WGV, 1999. Short fragment polymerase chain reaction reverse hybridization line probe assay to detect and genotype a broad spectrum of human papillomavirus types. Clinical evaluation and follow-up. Am J Pathol 155 : 1473–1478. [Google Scholar]
  10. Kleter B, van Doorn LJ, Schrauwen L, Molijn A, Sastrowijoto S, Schegget J, Lindeman J, Harmsel B, Burger M, Quint W, 1999. Development and clinical evaluation of a highly sensitive PCR-reverse hybridization line probe assay for detection and identification of anogenital human papillomavirus. J Clin Microbiol 37 : 2508–2517. [Google Scholar]
  11. Snijders PJ, Howewoning CJ, Hesselink AT, Berkhof J, Voothorst FJ, Bluker MC, Meijer CJ, 2006. Determination of viral load thresholds in cervical scrapings to rule out CIN3 in HPV 16, 18, 31 and 33 positive women with normal cytology. Int J Cancer 119 : 1102–1107. [Google Scholar]
  12. Carcopino X, Henry M, Benmoura D, Fallabregues AS, Richet H, Boubli L, Tamalet C, 2006. Determination of HPV type 16 and 18 viral load in cervical smears of women referred to colposcopy. J Med Virol 78 : 1131–1140. [Google Scholar]
  13. Gravitt PE, Peyton CL, Wheeler C, Apple R, Higuchi R, Shah KV, 2003. Reproducibility of HPV 16 and HPV 18 viral load quantitation using TaqMan real-time PCR assays. J Virol Methods 112 : 23–33. [Google Scholar]
  14. Weissenborn SJ, Funke AM, Hellmich M, Mallmann P, Fuchs PG, Pfister HJ, Wielandd U, 2003. Oncogenic human papillomavirus DNA loads in human immunodeficiency virus-positive women with high-grade cervical lesions are strongly elevated. J Clin Microbiol 41 : 2763–2767. [Google Scholar]
  15. Cox JT, Lorincz AT, Schiffman MH, Sherman ME, Cullen A, Kurman RJ, 1995. Human papillomavirus testing by Hybrid capture appears to be useful in triaging women with a cytologic diagnosis of atypical squamous cell of undetermined significance. Am J Obstet Gynecol 172 : 946–954. [Google Scholar]
  16. Hall S, Lorincz AT, Shah F, Sherman ME, Abbas F, Paull G, Kurman RJ, Shah K, 1996. Human papillomavirus DNA detection in cervical specimens by hybrid capture: correlation with cytologic and histologic diagnoses of squamous intraepithelial lesions of the cervix. Gynecol Oncol 62 : 353–359. [Google Scholar]
  17. Sun CA, Lai HC, Chang CC, Neih S, Yu CP, Chu TY, 2001. The significance of human papillomavirus viral load in prediction of histologic severity and size of squamous intraepithelial lesions of uterine cervix. Gynecol Oncol 83 : 95–99. [Google Scholar]
  18. Sun CA, Liu JF, Wu DM, Nieh S, Yu CP, Chu TY, 2002. Viral load of high-risk human papillomavirus in cervical squamous intraepithelial lesions. Int J Gynaecol Obstet 76 : 41–47. [Google Scholar]
  19. Dalstein V, Riethmuller D, Pretet JL, Le Bail Carval K, Sautiere JL, Carbillet JP, Kantelip B, Schaal JP, Mougin C, 2003. Persistence and load of high-risk HPV are predictors for development of high-grade cervical lesions: a longitudinal French cohort study. Int J Cancer 106 : 396–403. [Google Scholar]
  20. Swan DC, Tucker RA, Holloway BP, Icenogle JP, 1997. A sensitive, type-specific, fluorogenic probe assay for detection of human papillomavirus DNA. J Clin Microbiol 35 : 886–891. [Google Scholar]
  21. Josefsson A, Livak K, Gyllensten U, 1999. Detection and quantitation of human papillomavirus by using the fluorescent 5′ exonuclease assay. J Clin Microbiol 37 : 490–496. [Google Scholar]
  22. Peitsaro P, Johansson B, Syrjanen S, 2002. Integrated human papillomavirus type 16 is frequently found in cervical cancer precursors as demonstrated by a novel quantitative real-time PCR technique. J Clin Microbiol 40 : 886–891. [Google Scholar]
  23. Nagao S, Yoshinouchi M, Miyagi Y, Hongo A, Kodama J, Itoh S, Kudo T, 2002. Rapid and sensitive detection of physical status of human papillomavirus type 16 DNA by quantitative real-time PCR. J Clin Microbiol 40 : 863–867. [Google Scholar]
  24. Ho GY, Bierman R, Beardsley L, Chang CJ, Burk RD, 1998. Natural history of cervicovaginal papillomavirus infection in young women. N Engl J Med 338 : 423–428. [Google Scholar]
  25. Molano M, van den Brule A, Plummer M, Weiderpass E, Posso H, Arslan A, Meijer CJ, Muñoz N, Franceschi S, HPV Study Group, 2003. Determinants of clearance of human papillomavirus infections in Colombian women with normal cytology: a population-based, 5-year follow-up study. Am J Epidemiol 158 : 486–494. [Google Scholar]

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  • Received : 13 Jun 2007
  • Accepted : 21 Jan 2008

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