Volume 78, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Hemorrhagic fever with renal syndrome (HFRS) is a significant public health problem with an increasing incidence in Beijing, China (report of disease surveillance from the Center for Disease Control and Prevention of Beijing, China). Hantaviruses were detected using RT-PCR method in blood samples of HFRS patients and lung tissues of rodents captured in Beijing. Phylogenetic analyses of 724bp partial S segment of the hantavirus gene showed that the detected Seoul virus (SEOV) fell into three different lineages, two of which circulated in Beijing. A nucleotide sequence identity of 99.7% for one of the cases of HFRS—the human- and -originated SEOV sequences—had only two silent substitutions, suggesting genetic analysis is an essential tool for “case-investigation.”


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  1. Plyusnin A, Vapalahti O, Vaheri A, 1996. Hantavirus: genome structure, expression and evolution. J Gen Virol 77 : 2677–2687.
  2. Clement JP, 2003. Hantavirus. Antivir Res 57 : 121–127.
  3. Vapalahti O, Mustonen J, Lundkvist Å, Henttonen H, Plyusnin A, Antti V, 2003. Hantavirus infections in Europe. Lancet Infect Dis 3 : 653–661.
  4. ICTVdB, 2006. The Universal Virus Database. Version 4. Available at: http://www.ncbi.nlm.nih.gov/ICTVdb/.
  5. Hughes AL, Friedman R, 2000. Evolutionary diversification of protein-coding genes of Hantaviruses. Mol Biol Evol 7 : 1558–1568.
  6. Zeier M, Handermann M, Bahr U, Rensch B, Müller S, Kehm R, Murany W, Darai G, 2005. New ecological aspects of Hantavirus infection: a change of a paradigm and a challenge of prevention—a review. Virus Genes 30 : 157–180.
  7. Harta CA, Bennettb M, 1999. Hantavirus infections: epidemiology and pathogenesis. Microbes Infect 1 : 1229–1237.
  8. Song G, 1999. Epidemiological progresses of hemorrhagic fever with renal syndrome in China. Chin Med J (Engl) 112 : 472–477.
  9. Bai X, Huang C, 2002. Study farther on hemorrhagic fever with renal syndrome. Chin J Infect Dis 20 : 197–198.
  10. Zhang Y, Xiao D, Wang Y, Wang H, Sun L, Tao X, Qu Y, 2004. The epidemic characteristics and preventive measures of hemorrhagic fever with renal syndrome. Chin J Epidemiol 25 : 466–469.
  11. Zhang X, Zhou S, Wang H, Hu J, Guan Z, Liu H, 2000. Study on the genetic difference of SEO hantavirus. Chin J Epidemiol 21 : 349–351.
  12. Hu J, Li X, Xia H, Guan Z, 1990. Serologic investigation and isolation of hantavirus from rodents captured in Beijing. Beijing Med 12 : 321–324.
  13. Zhang X, Hu J, Zhou S, Guan Z, 2001. Study on epidemic state in Beijing. Chin J Vector Bio & Control 12 : 218–221.
  14. Zhang X, Hu J, Zhou S, Guan Z, 2001. Sero-type and clinical characteristics of the patients with HFRS in Beijing. Chin Public Health 17 : 220.
  15. Jiang J, Wu X, Zuo S, Xu W, Tang F, Wang B, Cai W, Dun Z, Cao W, 2004. Investigation on the rodents and their hantavirus infection in Beijing. Chin J Vector Bio & Control 15 : 458–460.
  16. Zuo S, Wu X, Sun P, Zhang P, Tang F, Zhan L, Wang B, Cai W, Dun Z, Yang H, Liu Q, Cao W, 2004. Study on the molecular epidemiology of hantavirus carried by hosts in northern suburb of Beijing. Chin J Epidemiol 25 : 421–424.
  17. Wang H, Yoshimatsu K, Ebihara H, Ogino M, Araki K, Kariwa H, Wang Z, Luo Z, Li D, Hang C, Arikawa J, 2000. Genetic diversity of hantavirus isolated in China and characterization of novel hantavirus isolated from Niviventer confucianus and Rattus rattus. Virology 278 : 332–345.
  18. Thompson JD, Higgins DG, Gibson TJ, Clustal W, 1994. Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res 22 : 4673–4680.
  19. Felsenstein J, 1993. PHYLIP: phylogeny interference package, version 3.65c. University of Washington, Seattle, Washington.
  20. Huson DH, Bryant D, 2006. Application of phylogenetic networks in evolutionary studies. Mol Biol Evol 23 : 254–267. Available at: www.splitstree.org.
  21. Wang S, Hang C, Wang H, Xie Y, Ma B, 2002. Genotype and clade distribution of hantaviruses in China. Chin J Virol 18 : 211–216.
  22. Shi X, McCaughey C, Elliott RM, 2003. Genetic characterization of a hantavirus isolated from a laboratory-acquired infection. J Med Virol 71 : 105–109.
  23. Hillis DM, Bul JJ, 1993. An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Syst Biol 42 : 182–192.
  24. Sun CQ, Chen LF, Wu YH, Tao WY, Liu YC, Cui Y, 2000. Molecular biological evidence of Seoul virus firstly isolated in the Northeast China (Heilongjiang Province): complete nucleotide sequence analysis of the S segment of hantavirus Pf26 strain. Chin J Control Prev 4 : 313–316.
  25. Hang C, Song G, Qiu X, Du Y, Zhao J, Liao H, Kong B, Gao G, Xu J, 1982. Etiological studies of EHF-related agent in endemic area of the mild type of hemorrhagic fever. Chin J Epidemiol 4 : 204–205.
  26. Nemirov K, Lundkvist Å, Vaheri A, Plyusnin A, 2003. Adaptation of Puumala hantavirus to cell culture is associated with point mutations in the coding region of the L segment and in the noncoding regions of the S segment. J Virol 77 : 8793–8800.

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  • Received : 03 Apr 2007
  • Accepted : 20 Jan 2008

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