Volume 78, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Four mosquito densovirus strains were assayed for mortality and infectivity against larvae from different geographic regions. The viral titers were quantified by real-time PCR using TaqMan technology. Firstinstar larvae were exposed to the same titer of each densovirus strain for 48 hours. All strains of densoviruses exhibited larvicidal activity and caused more than 80% mortality and infectivity in the three mosquito strains. DNV-exposed larvae had the highest mortality rate. The mean time to death of DNV-exposed larvae was shorter than other DNVs-exposed larvae. We can conclude that different densovirus strains exhibit some variations in their pathogenicity to different populations of mosquitoes. A few mosquitoes from Chachoengsao and Bangkok exposed to DNV and DNV survived to the adult stage to lay eggs and showed 22% to 50% vertical transmission in the F1 generation. Phylogenetic analysis of four densovirus strains indicated that mosquito densoviruses are separated into two distinct clades.


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  1. Somboon P, Prapanthadara LA, Suwonkerd W, 2003. Insecticide susceptibility tests of Anopheles minimus S.L., Aedes aegypti, Aedes albopictus, Culex quinquefasciatus in northern Thailand. Southeast Asian J Trop Med Public Health 30 : 184–194. [Google Scholar]
  2. Lima JBP, Da-Cunha MP, Júnior RCDS, Galardo AKR, Soares SDS, Braga IA, Ramos RP, Valle D, 2003. Resistance of Aedes aegypti to organophosphates in several municipalities in the state of Rio de Janeiro and Espírito Santo, Brazil. Am J Trop Med Hyg 68 : 329–333. [Google Scholar]
  3. Ponlawat A, Scott JG, Harrington LC, 2005. Insecticide susceptibility of Aedes aegypti and Aedes albopictus across Thailand. J Med Entomol 42 : 821–825. [Google Scholar]
  4. Bergoin M, Tijssen P, 2000. Molecular biology of densovirinae. Contrib Microbiol 4 : 12–32. [Google Scholar]
  5. Bando H, Choi H, Ito Y, Kawase S, 1990. Terminal structure of a densovirus implies a hairpin transfer replication which is similar to the model for AAV. Virology 179 : 57–6. [Google Scholar]
  6. Lebedeva PO, Zelenko AP, Kuznetsova MA, Gudzgorban AP, 1972. Studies on the demonstration of a viral infection in larvae of Aedes aegypti mosquitoes. Microbiol JUS 34 : 70–73. [Google Scholar]
  7. Buchatsky LP, 1989. Densonucleosis of bloodsucking mosquitoes. Dis Auq Org 6 : 145–150. [Google Scholar]
  8. Ledermann JP, Suchman EL, Black WC 4th, Carlson JO, 2004. Infection and pathogenicity of the mosquito densoviruses AeDNV, HeDNV, and APeDNV in Aedes aegypti mosquitoes (Diptera: Culicidae). J Econ Entomol 97 : 1828–1835. [Google Scholar]
  9. Jousset FX, Barreau C, Boublik Y, Cornet M, 1993. A parvo-like virus persistently infecting a C6/36 clone of Aedes albopictus mosquito cell line and pathogenic for Aedes aegypti larvae. Virus Res 29 : 99–114. [Google Scholar]
  10. Barreau C, Jousset FX, Cornet M, 1994. An efficient and easy method of infection of mosquito larvae from virus-contaminated cells cultures. J Virol Methods 49 : 153–156. [Google Scholar]
  11. Barreau C, Jousset FX, Bergoin M, 1996. Pathogenicity of the Aedes albopictus parvovirus (AaPV), a denso-like virus, for Aedes aegypti mosquitoes. J Invertebr Pathol 68 : 299–309. [Google Scholar]
  12. Kittayapong P, Baisley KJ, O’Neill SL, 1999. A mosquito denso-virus infecting Aedes aegypti and Aedes albopictus from Thailand. Am J Trop Med Hyg 61 : 612–617. [Google Scholar]
  13. Paterson A, Robinson E, Suchman EL, Afanasiev BN, Carlson JO, 2005. Mosquito densonucleosis viruses cause dramatically different infection phenotypes in the C6/36 Aedes albopictus cell line. Virology 337 : 253–261. [Google Scholar]
  14. Afanasiev BN, Kozlov YV, Carlson JO, Beaty BJ, 1994. Denso-virus of Aedes aegypti as an expression vector in mosquito cells. Exp Parasitol 79 : 322–339. [Google Scholar]
  15. Swofford DL, 1999. PAUP: Phylogenetic analysis using parsimony, version 4.0 betal. Centre for Agriculture and Bioscience International, Champaign, IL.
  16. Becnel JJ, 2006. Transmission of viruses to mosquito larvae mediated by divalent cations. J Invertebr Pathol 68 : 141–145. [Google Scholar]
  17. Wigglesworth VB, 1938. The regulation of osmotic pressure and chloride concentration in the hemolymph of mosquito larvae. J Exp Biol 15 : 235–247. [Google Scholar]
  18. Edwards HA, Harrison JB, 1983. An osmoregulatory syncytium and associated cells in a freshwater mosquito. Tissue Cell 15 : 271–280. [Google Scholar]
  19. Afanasiev BN, Ward TW, Beaty BJ, Carlson JO, 1999. Transduction of Aedes aegypti mosquitoes with vectors derived from Aedes densovirus. Virology 257 : 62–72. [Google Scholar]
  20. Allen-Muira TM, Afanasiev BN, Olson KE, Beaty BJ, Carlson JO, 1999. Packaging of AeDNV-GFP transducing virus by expression of densoviral structural proteins from a sinbis virus expression system. Virology 257 : 54–61. [Google Scholar]
  21. Ward TW, Jenkins MS, Afanasiev BN, Edwards M, Duda BA, Suchman E, Jacobs-Lorena M, Beaty BJ, Carlson JO, 2001. Aedes aegypti transducing densovirus pathogenesis and expression in Aedes aegypti and Anopheles gambiae larvae. Insect Mol Biol 10 : 397–405. [Google Scholar]
  22. Roekring S, Flegel TW, Malasit P, Kittayapong P, 2006. Challenging successive mosquito generations with a densonucleosis virus yields progressive survival improvement but persistent, innocuous infections. Dev Comp Immunol 30 : 878–892. [Google Scholar]
  23. Boublik Y, Jousset FX, Bergoin M, 1994. Complete nucleotide sequence and genomic organization of the Aedes albopictus parvovirus (AaPV) pathogenic for Aedes aegypti larvae. Virology 200 : 752–763. [Google Scholar]
  24. O’Neill SL, Kittayapong P, Braig HR, Andreadis TG, Gonzalez JP, Tesh RB, 1995. Insect densoviruses may be widespread in mosquito cell lines. J Gen Virol 76 : 2067–2074. [Google Scholar]
  25. Chen S, Cheng L, Zhang Q, Lin W, Lu X, Brannan J, Zhou ZH, Zhang J, 2004. Genetic, biochemical, and structural characterization of a new densovirus isolated from chronically infected Aedes albopictus C6/36 cell lines. Virology 318 : 123–133. [Google Scholar]
  26. Becker N, Zgomba M, Petri D, Beck M, Ludwig M, 1995. Role of larval cadavers in recycling processes of Bacillus sphaericus. J Am Mosq Contr Assoc 11 : 329–334. [Google Scholar]
  27. Suchman EL, Kononko A, Plake E, Doehling M, Kleker B, Black WC 4th, Buchatsky L, Carlson JO, 2006. Effects of AeDNV infection on Aedes aegypti lifespan and reproduction. Biol Control 39 : 465–473. [Google Scholar]
  28. Barreau C, Jousset FX, Bergoin M, 1997. Venereal and vertical transmission of the Aedes albopictus parvovirus in Aedes aegypti mosquitoes. Am J Trop Med Hyg 57 : 126–131. [Google Scholar]

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  • Received : 19 May 2007
  • Accepted : 30 Dec 2007

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