Volume 78, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


This study describes the isolation and characterization of several species from soil in northern Australia. Phenotypic and molecular tests indicate that these isolates belong to the species and These observations significantly extend our knowledge of the geographic distribution of these 2 species. Evidence of these species in Australia has implications for bacterial identification in clinical laboratories, diagnostic serology tests, and environmental biodiversity studies.


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  1. Brett P, DeShazer D, Woods D, 1998. Burkholderia thailandensis sp. nov., a Burkholderia pseudomallei-like species. Int J Syst Bacteriol 48 : 317–320. [Google Scholar]
  2. Glass MB, Steigerwalt AG, Jordan JG, Wilkins PP, Gee JE, 2006. Burkholderia oklahomensis sp. nov., a Burkholderia pseudomallei-like species formerly known as the Oklahoma strain of Pseudomonas pseudomallei. Int J Syst Evol Microbiol 56 : 2171–2176. [Google Scholar]
  3. Yabuuchi E, Kawamura Y, Ezaki T, Ikedo M, Dejsirilert S, Fujiwara N, Nake T, Kobayashi K, 2000. Burkholderia uboniae sp. nov. l-arabinose-assimilating but different from Burkholderia thailandensis and Burkholderia vietnamiensis. Microbiol Immunol 44 : 307–317. [Google Scholar]
  4. Payne GW, Vandamme P, Morgan SH, Lipuma JJ, Coenye T, Weightman AJ, Jones TH, Mahenthiralingam E, 2005. Development of a recA gene-based identification approach for the entire Burkholderia genus. Appl Environ Microbiol 71 : 3917–3927. [Google Scholar]
  5. Glass MB, Gee JE, Steigerwalt AG, Cavuoti D, Barton T, Hardy RD, Godoy D, Spratt BG, Clark TA, Wilkins PP, 2006. Pneumonia and septicemia caused by Burkholderia thailandensis from the United States. J Clin Microbiol 44 : 4601–4604. [Google Scholar]
  6. Lertpatanasuwan N, Sermsri K, Petkaseam A, Trakulsomboon S, Thamlikitkul V, Suputtamongkol Y, 1999. Arabinose-positive Burkholderia pseudomallei infection in humans: case report. Clin Infect Dis 28 : 927–928. [Google Scholar]
  7. Inglis TJ, Foster NF, Gal D, Powell K, Mayo M, Norton R, Currie BJ, 2004. Preliminary report on the northern Australian melioidosis environmental surveillance project. Epidemiol Infect 132 : 813–820. [Google Scholar]
  8. Chantratita N, Wuthiekanun V, Thanwisai A, Limmathurotsakul D, Cheng AC, Chierakul W, Day NP, Peacock SJ, 2007. Accuracy of enzyme-linked immunosorbent assay using crude and purified antigens for serodiagnosis of melioidosis. Clin Vaccine Immunol 14 : 110–113. [Google Scholar]
  9. Tiyawisutsri R, Peacock SJ, Langa S, Limmathurotsakul D, Cheng AC, Chierakul W, Chaowagul W, Day NP, Wuthiekanun V, 2005. Antibodies from patients with melioidosis recognize Burkholderia mallei but not Burkholderia thailandensis antigens in the indirect hemagglutination assay. J Clin Microbiol 43 : 4872–4874. [Google Scholar]
  10. Wuthiekanun V, Smith MD, Dance DA, Walsh AL, Pitt TL, White NJ, 1996. Biochemical characteristics of clinical and environmental isolates of Burkholderia pseudomallei. J Med Microbiol 45 : 408–412. [Google Scholar]
  11. Weyant RS, Moss CW, Weaver RE, Hollis DG, Jorda JG, Cook EC, Daneshvar MI, 1996. Media and methods. Hensyl WR, ed. Identification of Unusual Pathogenic Gram-Negative Aerobic and Facultatively Anaerobic Bacteria. Baltimore, MD: Williams & Wilkins, 14–15.
  12. Inglis TJ, Aravena-Roman M, Ching S, Croft K, Wuthiekanun V, Mee BJ, 2003. Cellular fatty acid profile distinguishes Burkholderia pseudomallei from avirulent Burkholderia thailandensis. J Clin Microbiol 41 : 4812–4814. [Google Scholar]
  13. Coenye T, Lipuma JJ, 2002. Use of the gyrB gene for the identification of Pandoraea species. FEMS Microbiol Lett 208 : 15–19. [Google Scholar]
  14. Inglis TJ, Merritt A, Chidlow G, Aravena-Roman M, Harnett G, 2005. Comparison of diagnostic laboratory methods for identification of Burkholderia pseudomallei. J Clin Microbiol 43 : 2201–2206. [Google Scholar]
  15. Kunakorn M, Markham RB, 1995. Clinically practical seminested PCR for Burkholderia pseudomallei quantitated by enzyme immunoassay with and without solution hybridization. J Clin Microbiol 33 : 2131–2135. [Google Scholar]
  16. Merritt A, Inglis TJ, Chidlow G, Harnett G, 2006. PCR- based identification of Burkholderia pseudomallei. Rev Inst Med Trop S Paulo 48 : 239–244. [Google Scholar]
  17. PulseNet USA: The Molecular Subtyping Network for Food-borne Bacterial Disease Surveillance, 2004. One-day (24–28 h) standardized laboratory protocol for molecular subtyping of Escherichia coli O157:H7, non-typhoidal Salmonella serotypes, and Shigella sonnei by pulsed field gel electrophoresis (PFGE). PulseNet Protocols. Atlanta, GA: Centers for Disease Control. Available at: http://www.cdc.gov/PULSENET/protocols/ecoli_salmonella_shigella_protocols.pdf.
  18. Inglis TJ, Garrow SC, Adams C, Henderson M, Mayo M, Currie BJ, 1999. Acute melioidosis outbreak in Western Australia. Epidemiol Infect 123 : 437–443. [Google Scholar]
  19. Godoy D, Randle G, Simpson AJ, Aanensen DM, Pitt TL, Kinoshita R, Spratt BG, 2003. Multilocus sequence typing and evolutionary relationships among the causative agents of melioidosis and glanders, Burkholderia pseudomallei and Burkholderia mallei. J Clin Microbiol 41 : 2068–2079. [Google Scholar]
  20. Jolley K, Chan M-S, Maiden M, 2004. mlstdbNet— distributed multilocus sequence typing (MLST) databases. BMC Bioinformatics 5 : 86. [Google Scholar]
  21. Cheng AC, O’Brien M, Freeman K, Lum G, Currie BJ, 2006. Indirect hemagglutination assay in patients with melioidosis in Northern Australia. Am J Trop Med Hyg 74 : 330–334. [Google Scholar]
  22. Gilmore G, Barnes J, Ketheesan N, Norton R, 2007. Use of antigens derived from Burkholderia pseudomallei, B. thailandensis, and B. cepacia in the direct hemagglutination assay for melioidosis. Clin Vaccine Immunol. 14 : 1529–1531. [Google Scholar]

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  • Received : 09 Nov 2007
  • Accepted : 26 Jan 2008

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