1921
Volume 78, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

We developed real-time fluorescence resonance energy transfer (FRET) polymerase chain reaction (PCR) combined with melting curve analysis for detection of DNA in blood-fed mosquitoes. Real-time FRET PCR is based on a fluorescence melting curve analysis of hybrid formed between amplicons generated from a family of repeated DNA element, 153-bp I repeated sequence, specific to genus and specific fluorophore-labeled probes. The –infected mosquitoes were differentiated from –infected and uninfected mosquitoes and from genomic DNA of – and –infected human red blood cells and human leukocytes by their melting temperature. Sensitivity and specificity were both 100%. Melting curve analysis produces a rapid, accurate, and sensitive alternative for specific detection of in mosquitoes, allows high throughput, and can be performed on small samples. This method has the potential for endemic area mapping or monitoring effect of brugian filariasis mass treatment programs.

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  • Received : 10 Oct 2007
  • Accepted : 08 Dec 2007

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