Volume 78, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Most congenital transmissions of are not detected. As the levels of mediators regulating the immune response might be different in the absence or in the presence of transmission, we explored the levels of tumor necrosis factor (TNF) and soluble TNF receptors TNF-R1 and -R2 in –infected pregnant women and the neonates. We previously found that the circulating levels of TNF were higher in non-transmitting than in transmitting pregnant women. This observation has now been extended to the spontaneous release of TNF by peripheral blood leukocytes (PBLs) that was also higher in non-transmitting than in transmitting pregnant women. As their mothers, non-infected neonates had higher circulating levels of TNF than congenitally infected children. The circulating levels of sTNF-R1 increased in non-transmitting and transmitting mothers and in infected and non-infected neonates. The circulating levels of sTNF-R2 were ∼60% higher in infected than in non-infected neonates (1,635 ± 101 and 1,027 ± 100 pg/mL, respectively) and remained higher at 1 year of age. This important increase, only observed in infected neonates, could be useful to orientate to the presence of vertical transmission of infection.


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  1. Schmuñis GA, 1999. A Tripanossomiase Americana e seu impacto na saúde pública das Americas. Brener Z, Andrade ZA, Barral Neto M, eds. Trypanosoma cruzi e Doenca de Chagas. Rio de Janeiro: Guanabara Koogan, 1–15.
  2. Gürtler RE, Segura EL, Cohen JE, 2003. Congenital transmission of Trypanosoma cruzi infection in Argentina. Emerg Infect Dis 9 : 29–32. [Google Scholar]
  3. Arcavi M, Orfus G, Griemberg G, 1993. Incidencia de la infección chagásica en embarazadas y recién nacidos en área no endémica. Medicina (B Aires) 53 : 217–222. [Google Scholar]
  4. Storni P, Bolsi FL, 1979. Embarazo y parasitismo por Trypanosoma cruzi. Medicina (B Aires) 39 : 193–197. [Google Scholar]
  5. Szarfman A, Urman J, Otalova A, Larzuia A, Yanovsky JF, 1975. Specific agglutinins and immunoglobulin levels in congenital Chagas disease. Medicina (B Aires) 35 : 245–250. [Google Scholar]
  6. Votta RA, Marchese CA, Pastorini A, Gonzalez CA, Lautrec L, Tomassini TL, 1977. Multimetodología diagnóstica de la enfermedad de Chagas Mazza congénita en un medio no endémico. Revista de la Sociedad de Obstetricia y Ginecología de Buenos Aires 56 : 237–250. [Google Scholar]
  7. Freilij H, Altcheh J, 1995. Congenital Chagas disease: diagnosis and clinical aspects. Clin Infect Dis 21 : 551–555. [Google Scholar]
  8. Hermann E, Truyens C, Alonso Vega C, Rodriguez P, Berthe A, Torrico F, Carlier Y, 2004. Congenital transmission of Trypanosoma cruzi is associated with maternal enhanced parasitemia and decreased production of Interferon-γ in response to parasite antigens. J Infect Dis 189 : 1274–1281. [Google Scholar]
  9. Santos Lima EC, García I, Vicentelli MH, Vassalli P, Minoprio P, 1997. Evidence for a protective role of tumor necrosis factor in the acute phase of Trypanosoma cruzi infection in mice. Infect Immun 65 : 457–465. [Google Scholar]
  10. Hölscher C, Köhler G, Müller U, Mossmann H, Schaub GA, Brombacher F, 1998. Defective nitric oxide effector functions lead to extreme susceptibility of Trypanosoma cruzi-infected mice deficient in gamma interferon receptor or inducible nitric oxide synthase. Infect Immun 66 : 1208–1215. [Google Scholar]
  11. Köberle F, 1968. Chagas’ disease and Chagas’ syndromes: the pathology of American trypanosomiasis. Adv Parasitol 6 : 63–116. [Google Scholar]
  12. Perez-Fuentes R, Guegan JF, Barnabe C, Lopez-Colombo A, Salgado-Rosas H, Torres-Rasgado E, Briones B, Romero-Díaz M, Ramos-Jimenez J, Sánchez-Guillen M del C, 2003. Severity of chronic Chagas disease is associated with cytokine/antioxidant imbalance in chronically infected individuals. Int J Parasitol 33 : 293–299. [Google Scholar]
  13. Derouich-Guergour D, Brenier-Pinchart MP, Ambroise-Thomas P, Pelloux H, 2001. Tumour necrosis factor α receptors: role in the physiopathology of protozoan parasite infections. Int J Parasitol 31 : 763–769. [Google Scholar]
  14. Cardoni RL, García MM, De Rissio AM, 2004. Proinflammatory and anti-inflammatory cytokines in pregnant women chronically infected with Trypanosoma cruzi. Acta Trop 90 : 65–72. [Google Scholar]
  15. Cura EN, Segura EL, 1998. Quality assurance of the serologic diagnosis of Chagas disease. Rev Panam Salud Publica/Pan Am/Public Health 3 : 242–248. [Google Scholar]
  16. Guía para la atención al paciente infectado con Trypanosoma cruzi. Ministerio de Salud, 2006. Resolución 1870. Available at: www.msal.gov.ar/htm/site/chagas. Accessed August 10, 2007.
  17. Svahn A, Linde A, Thorstensson R, Karlen K, Andersson L, Gaines H, 2003. Development and evaluation of a flow-cytometric assay of specific cell-mediated immune response in activated whole blood for the detection of cell-mediated immunity against varicella-zoster virus. J Immunol Methods 277 : 17–25. [Google Scholar]
  18. Leeuwenberg JF, Jeunhomme TM, Buurman WA, 1994. Slow release of soluble TNF receptors by monocytes in vitro. J Immunol 152 : 4036–4043. [Google Scholar]
  19. Joyce DA, Gibbons DP, Green P, Steer JH, Feldmann M, Brennan FM, 1994. Two inhibitors of pro-inflammatory cytokine release, interleukin-10 and interleukin-4, have contrasting effects on release of soluble p75 tumor necrosis factor receptor by cultured monocytes. Eur J Immunol 24 : 2699–2705. [Google Scholar]
  20. Raghupathy R, 1997. Th1-type immunity is incompatible with successful pregnancy. Immunol Today 18 : 478–482. [Google Scholar]
  21. Steinborn A, Gunes H, Halberstadt E, 1995. Signal for term parturition is of trophoblast and therefore of fetal origin. Prostaglandins 50 : 237–252. [Google Scholar]
  22. Steinborn A, Gunes H, Roddiger S, Halberstadt E, 1996. Elevated placental cytokine release, a process associated with preterm labor in the absence of intrauterine infection. Obstet Gynecol 88 : 534–539. [Google Scholar]
  23. Joyce DA, Steer JH, Abraham LJ, 1997. Glucocorticoid modulation of human monocyte/macrophage function: control of TNF-αsecretion. Inflamm Res 46 : 447–451. [Google Scholar]
  24. Snyder SK, Wessner DH, Wessells JL, Waterhouse RM, Wahl LM, Zimmermann W, Dveksler GS, 2001. Pregnancy-specific glycoproteins function as immunomodulators by inducing secretion of IL-10, IL-6 and TGF-β1 by human monocytes. Am J Reprod Immunol 45 : 205–216. [Google Scholar]
  25. Chernyshov VP, Vodyanik MA, Pisareva SP, 2005. Lack of soluble TNF-receptors in women with recurrent spontaneous abortion and possibility for its correction. Am J Reprod Immunol 54 : 284–291. [Google Scholar]
  26. Vekemans J, Truyens C, Torrico F, Solano M, Torrico MC, Rodriguez P, Alonso-Vega C, Carlier Y, 2000. Maternal Trypanosoma cruzi infection upregulates capacity of uninfected neonate cells to produce pro- and anti-inflammatory cytokines. Infect Immun 68 : 5430–5434. [Google Scholar]
  27. Kwiatkowski D, Hill AV, Sambou I, Twumasi P, Castracane J, Manogue KR, Cerami A, Brewster DR, Greenwood BM, 1990. TNF concentration in fatal cerebral, non-fatal cerebral, and uncomplicated Plasmodium falciparum malaria. Lancet 336 : 1201–1204. [Google Scholar]
  28. Zwingenberger K, Harms G, Pedrosa C, Pessoa MC, Sandkamp B, Scheibenbogen C, Andreesen R, 1991. Generation of cytokines in human visceral leishmaniasis: dissociation of endogenous TNF-α and IL-1β production. Immunobiology 183 : 125–132. [Google Scholar]
  29. Shobokshi A, Shaarawy M, 2002. Maternal serum and amniotic fluid cytokines in patients with preterm premature rupture of membranes with and without intrauterine infection. Int J Gynaecol Obstet 79 : 209–215. [Google Scholar]
  30. Van Zee KJ, Kohno T, Fischer E, Rock CS, Moldawer LL, Lowry SF, 1992. Tumor necrosis factor soluble receptors circulate during experimental and clinical inflammation and can protect against excessive tumor necrosis factor α in vitro and in vivo. Proc Natl Acad Sci USA 89 : 4845–4849. [Google Scholar]
  31. Austgulen R, Liabakk NB, Brockhaus M, Espevik T, 1992. Soluble TNF receptors in amniotic fluid and in urine from pregnant women. J Reprod Immunol 22 : 105–116. [Google Scholar]
  32. Doellner H, Arntzen KJ, Haereid PE, Aag S, Brubakk AM, Austgulen R, 1998. Increased serum concentrations of soluble tumor necrosis factor receptors p55 and p75 in early onset neonatal sepsis. Early Hum Dev 52 : 251–261. [Google Scholar]

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  • Received : 16 Aug 2007
  • Accepted : 28 Nov 2007

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