Volume 78, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


We evaluated a PCR-RFLP of the ribosomal internal transcribed spacer 2 region (ITS2) to distinguish species of commonly reported in the Amazon and validated this method using reared F offspring. The following species of were used for molecular analysis: , and (s.l.). In addition, three species of the subgenus , , , and were included for testing. Each of the nine species tested yielded diagnostic banding patterns. The PCR-RFLP method was successful in identifying all life stages including exuviae with small fractions of the sample. The assay is rapid and can be applied as an unbiased confirmatory method for identification of morphologic variants, disputed samples, imperfectly preserved specimens, and life stages from which taxonomic keys do not allow for definitive species determination.


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  • Received : 01 Sep 2007
  • Accepted : 10 Dec 2007

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